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Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes

L Galluzzi, SA Aaronson, J Abrams, ES Alnemri, DW Andrews, EH Baehrecke, NG Bazan, MV Blagosklonny, K Blomgren, C Borner, et al. (2009) CELL DEATH AND DIFFERENTIATION. 16(8). p.1093-1107
abstract
Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
keyword
INDUCED THYMOCYTE APOPTOSIS, RELEASE, MITOCHONDRIAL-MEMBRANE PERMEABILIZATION, CYTOCHROME-C, CANCER-CELLS, FLOW-CYTOMETRY, OXIDATIVE STRESS, AUTOPHAGY, NUCLEAR-DNA, DROSOPHILA
journal title
CELL DEATH AND DIFFERENTIATION
Cell Death Differ.
volume
16
issue
8
pages
15 pages
publisher
NATURE PUBLISHING GROUP
place of publication
LONDON
Web of Science type
Review
Web of Science id
000267948900004
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
8.24 (2009)
JCR rank
23/281 (2009)
JCR quartile
1 (2009)
ISSN
1350-9047
DOI
10.1038/cdd.2009.44
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
787247
handle
http://hdl.handle.net/1854/LU-787247
date created
2009-11-23 13:36:55
date last changed
2017-01-02 09:55:26
@article{787247,
  abstract     = {Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases.
Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied
biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of
utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in
tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been
proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and
nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we
provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their
advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need
to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number
of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of
performing multiple, methodologically unrelated assays to quantify dying and dead cells.},
  author       = {Galluzzi, L and Aaronson, SA and Abrams, J and Alnemri, ES and Andrews, DW and Baehrecke, EH and Bazan, NG and Blagosklonny, MV and Blomgren, K and Borner, C and Bredesen, DE and Brenner, C and Castedo, M and Cidlowski, JA and Ciechanover, A and Cohen, GM and De Laurenzi, V and De Maria, R and Deshmukh, M and Dynlacht, BD and El-Deiry, WS and Flavell, RA and Fulda, S and Garrido, C and Golstein, P and Gougeon, ML and Green, DR and Gronemeyer, H and Hajnoczky, G and Hardwick, JM and Hengartner, MO and Ichijo, H and Jaattela, M and Kepp, O and Kimchi, A and Klionsky, DJ and Knight, RA and Kornbluth, S and Kumar, S and Levine, B and Lipton, SA and Lugli, E and Madeo, F and Malorni, W and Marine, Jean-Christophe and Martin, SJ and Medema, JP and Mehlen, P and Melino, G and Moll, UM and Morselli, E and Nagata, S and Nicholson, DW and Nicotera, P and Nunez, G and Oren, M and Penninger, J and Pervaiz, S and Peter, ME and Piacentini, M and Prehn, JHM and Puthalakath, H and Rabinovich, GA and Rizzuto, R and Rodrigues, CMP and Rubinsztein, DC and Rudel, T and Scorrano, L and Simon, HU and Steller, H and Tschopp, J and Tsujimoto, Y and Vandenabeele, Peter and Vitale, I and Vousden, KH and Youle, RJ and Yuan, J and Zhivotovsky, B and Kroemer, G},
  issn         = {1350-9047},
  journal      = {CELL DEATH AND DIFFERENTIATION},
  keyword      = {INDUCED THYMOCYTE APOPTOSIS,RELEASE,MITOCHONDRIAL-MEMBRANE PERMEABILIZATION,CYTOCHROME-C,CANCER-CELLS,FLOW-CYTOMETRY,OXIDATIVE STRESS,AUTOPHAGY,NUCLEAR-DNA,DROSOPHILA},
  language     = {eng},
  number       = {8},
  pages        = {1093--1107},
  publisher    = {NATURE PUBLISHING GROUP},
  title        = {Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes},
  url          = {http://dx.doi.org/10.1038/cdd.2009.44},
  volume       = {16},
  year         = {2009},
}

Chicago
Galluzzi, L, SA Aaronson, J Abrams, ES Alnemri, DW Andrews, EH Baehrecke, NG Bazan, et al. 2009. “Guidelines for the Use and Interpretation of Assays for Monitoring Cell Death in Higher Eukaryotes.” Cell Death and Differentiation 16 (8): 1093–1107.
APA
Galluzzi, L, Aaronson, S., Abrams, J., Alnemri, E., Andrews, D., Baehrecke, E., Bazan, N., et al. (2009). Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. CELL DEATH AND DIFFERENTIATION, 16(8), 1093–1107.
Vancouver
1.
Galluzzi L, Aaronson S, Abrams J, Alnemri E, Andrews D, Baehrecke E, et al. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. CELL DEATH AND DIFFERENTIATION. LONDON: NATURE PUBLISHING GROUP; 2009;16(8):1093–107.
MLA
Galluzzi, L, SA Aaronson, J Abrams, et al. “Guidelines for the Use and Interpretation of Assays for Monitoring Cell Death in Higher Eukaryotes.” CELL DEATH AND DIFFERENTIATION 16.8 (2009): 1093–1107. Print.