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Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

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Abstract
Factors affecting conversion of horse chestnut (A. hippocastanum L.) somatic embryos into plantlets were evaluated. Anther filament derived embryogenic tissue developed bipolar structures with two cotyledons and a well-developed shoot and root apical meristem upon auxin omittance from the culturing medium. The impact of carbohydrate type (glucose, fructose, sucrose and maltose) and concentration (3 and 6%) on somatic embryo maturation and conversion were evaluated. Although conversion frequencies were high for all treatments, overall quality of regenerated plantlets was poor. Increasing the carbohydrate concentration in the maturation medium did not increase conversion of somatic embryos or quality of regenerated plantlets in terms of shoot height. On the contrary, addition of PEG (polyethylene glycol) in maturation media had a beneficial effect on shoot quality of regenerated plantlets. Sucrose was a superior carbon source when PEG was included in the maturation medium, in terms of conversion rate (65.7%) as well as of shoot quality of plantlets (43.8% of plantlets had shoots > 2 cm). Clonal fidelity of the different development stages of somatic embryogenesis and of converted plantlets was assessed by flow cytometry and no major ploidy changes were found.
Keywords
GERMINATION, ABSCISIC-ACID, MATURATION, Histology, Ploidy stability, Osmoticum, Maturation, Somatic embryogenesis, Aesculus hippocastanum, NUCLEAR-DNA CONTENT, POLYETHYLENE-GLYCOL, OSMOTIC-PRESSURE, FLOW-CYTOMETRY, STEM EXPLANTS, EMBRYOGENESIS, LEAF EXPLANTS

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Citation

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Chicago
Troch, Veronique, Stefaan Werbrouck, Danny Geelen, and Marie-Christine Van Labeke. 2009. “Optimization of Horse Chestnut (Aesculus Hippocastanum L.) Somatic Embryo Conversion.” Plant Cell Tissue and Organ Culture 98 (1): 115–123.
APA
Troch, V., Werbrouck, S., Geelen, D., & Van Labeke, M.-C. (2009). Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion. PLANT CELL TISSUE AND ORGAN CULTURE, 98(1), 115–123.
Vancouver
1.
Troch V, Werbrouck S, Geelen D, Van Labeke M-C. Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion. PLANT CELL TISSUE AND ORGAN CULTURE. 2009;98(1):115–23.
MLA
Troch, Veronique, Stefaan Werbrouck, Danny Geelen, et al. “Optimization of Horse Chestnut (Aesculus Hippocastanum L.) Somatic Embryo Conversion.” PLANT CELL TISSUE AND ORGAN CULTURE 98.1 (2009): 115–123. Print.
@article{783296,
  abstract     = {Factors affecting conversion of horse chestnut (A. hippocastanum L.) somatic embryos into plantlets were evaluated. Anther filament derived embryogenic tissue developed bipolar structures with two cotyledons and a well-developed shoot and root apical meristem upon auxin omittance from the culturing medium. The impact of carbohydrate type (glucose, fructose, sucrose and maltose) and concentration (3 and 6\%) on somatic embryo maturation and conversion were evaluated. Although conversion frequencies were high for all treatments, overall quality of regenerated plantlets was poor. Increasing the carbohydrate concentration in the maturation medium did not increase conversion of somatic embryos or quality of regenerated plantlets in terms of shoot height. On the contrary, addition of PEG (polyethylene glycol) in maturation media had a beneficial effect on shoot quality of regenerated plantlets. Sucrose was a superior carbon source when PEG was included in the maturation medium, in terms of conversion rate (65.7\%) as well as of shoot quality of plantlets (43.8\% of plantlets had shoots {\textrangle} 2 cm). Clonal fidelity of the different development stages of somatic embryogenesis and of converted plantlets was assessed by flow cytometry and no major ploidy changes were found.},
  author       = {Troch, Veronique and Werbrouck, Stefaan and Geelen, Danny and Van Labeke, Marie-Christine},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {GERMINATION,ABSCISIC-ACID,MATURATION,Histology,Ploidy stability,Osmoticum,Maturation,Somatic embryogenesis,Aesculus hippocastanum,NUCLEAR-DNA CONTENT,POLYETHYLENE-GLYCOL,OSMOTIC-PRESSURE,FLOW-CYTOMETRY,STEM EXPLANTS,EMBRYOGENESIS,LEAF EXPLANTS},
  language     = {eng},
  number       = {1},
  pages        = {115--123},
  title        = {Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion},
  url          = {http://dx.doi.org/10.1007/s11240-009-9544-8},
  volume       = {98},
  year         = {2009},
}

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