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Nitric oxide production by the human intestinal microbiota by dissimilatory nitrate reduction to ammonium

Joan Vermeiren (UGent) , Tom Van de Wiele (UGent) , Willy Verstraete (UGent) , Pascal Boeckx (UGent) and Nico Boon (UGent)
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Abstract
The free radical nitric oxide (NO) is an important signaling molecule in the gastrointestinal tract. Besides eukaryotic cells, gut microorganisms are also capable of producing NO. However, the exact mechanism of NO production by the gut microorganisms is unknown. Microbial NO production was examined under in vitro conditions simulating the gastrointestinal ecosystem using L-arginine or nitrate as substrates. L-arginine did not influence the microbial NO production. However, NO concentrations in the order of 90 ng NO-N per L feed medium were produced by the fecal microbiota from nitrate. N-15 tracer experiments showed that nitrate was mainly reduced to ammonium by the dissimilatory nitrate reduction to ammonium (DNRA) pathway. To our knowledge, this is the first study showing that gastrointestinal microbiota can generate substantial amounts of NO by DNRA and not by the generally accepted denitrification or L-arginine pathway. Further work is needed to elucidate the exact role between NO produced by the gastrointestinal microbiota and host cells.
Keywords
COMPLETE GENOME SEQUENCE, INFLAMMATORY-BOWEL-DISEASE, BACILLUS-SUBTILIS, DEINOCOCCUS-RADIODURANS, EPITHELIAL PERMEABILITY, STAPHYLOCOCCUS-AUREUS, L-ARGININE, SYNTHASE, BACTERIA, DENITRIFICATION

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Chicago
Vermeiren, Joan, Tom Van de Wiele, Willy Verstraete, Pascal Boeckx, and Nico Boon. 2009. “Nitric Oxide Production by the Human Intestinal Microbiota by Dissimilatory Nitrate Reduction to Ammonium.” Journal of Biomedicine and Biotechnology.
APA
Vermeiren, J., Van de Wiele, T., Verstraete, W., Boeckx, P., & Boon, N. (2009). Nitric oxide production by the human intestinal microbiota by dissimilatory nitrate reduction to ammonium. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.
Vancouver
1.
Vermeiren J, Van de Wiele T, Verstraete W, Boeckx P, Boon N. Nitric oxide production by the human intestinal microbiota by dissimilatory nitrate reduction to ammonium. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. 2009;
MLA
Vermeiren, Joan, Tom Van de Wiele, Willy Verstraete, et al. “Nitric Oxide Production by the Human Intestinal Microbiota by Dissimilatory Nitrate Reduction to Ammonium.” JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2009): n. pag. Print.
@article{780316,
  abstract     = {The free radical nitric oxide (NO) is an important signaling molecule in the gastrointestinal tract. Besides eukaryotic cells, gut microorganisms are also capable of producing NO. However, the exact mechanism of NO production by the gut microorganisms is unknown. Microbial NO production was examined under in vitro conditions simulating the gastrointestinal ecosystem using L-arginine or nitrate as substrates. L-arginine did not influence the microbial NO production. However, NO concentrations in the order of 90 ng NO-N per L feed medium were produced by the fecal microbiota from nitrate. N-15 tracer experiments showed that nitrate was mainly reduced to ammonium by the dissimilatory nitrate reduction to ammonium (DNRA) pathway. To our knowledge, this is the first study showing that gastrointestinal microbiota can generate substantial amounts of NO by DNRA and not by the generally accepted denitrification or L-arginine pathway. Further work is needed to elucidate the exact role between NO produced by the gastrointestinal microbiota and host cells.},
  author       = {Vermeiren, Joan and Van de Wiele, Tom and Verstraete, Willy and Boeckx, Pascal and Boon, Nico},
  issn         = {1110-7243},
  journal      = {JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY},
  keyword      = {COMPLETE GENOME SEQUENCE,INFLAMMATORY-BOWEL-DISEASE,BACILLUS-SUBTILIS,DEINOCOCCUS-RADIODURANS,EPITHELIAL PERMEABILITY,STAPHYLOCOCCUS-AUREUS,L-ARGININE,SYNTHASE,BACTERIA,DENITRIFICATION},
  language     = {eng},
  title        = {Nitric oxide production by the human intestinal microbiota by dissimilatory nitrate reduction to ammonium},
  url          = {http://dx.doi.org/10.1155/2009/284718},
  year         = {2009},
}

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