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Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium

Thomas Geurden (UGent) , Bruno Levecke (UGent) , SM Caccio, Aline Visser (UGent) , G De Groote, Stijn Casaert (UGent) , Jozef Vercruysse (UGent) and Edwin Claerebout (UGent)
(2009) PARASITOLOGY. 136(10). p.1161-1168
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Abstract
Stool samples from Belgian patients Suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54.2% and C. parvum 45.8% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IlaA15G2R1 of C. parvum were the most prevalent, although several other subgenotypes were identified. For Giardia, sequencing at the beta-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74.4%) in human patients. A high degree of heterogeneity was found, especially on the beta-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32.4%) of human patients, which might have important epidemiological implications.
Keywords
ZOONOTIC TRANSMISSION, DUODENALIS, MOLECULAR EPIDEMIOLOGY, risk factors, species-specific, multilocus, sporadic, prevalence, human, Giardia, Cryptosporidium, CALVES, SUBGENOTYPES, SUBTYPES, PARVUM, CHILDREN

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Chicago
Geurden, Thomas, Bruno Levecke, SM Caccio, Aline Visser, G De Groote, Stijn Casaert, Jozef Vercruysse, and Edwin Claerebout. 2009. “Multilocus Genotyping of Cryptosporidium and Giardia in Non-outbreak Related Cases of Diarrhoea in Human Patients in Belgium.” Parasitology 136 (10): 1161–1168.
APA
Geurden, Thomas, Levecke, B., Caccio, S., Visser, A., De Groote, G., Casaert, S., Vercruysse, J., et al. (2009). Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium. PARASITOLOGY, 136(10), 1161–1168.
Vancouver
1.
Geurden T, Levecke B, Caccio S, Visser A, De Groote G, Casaert S, et al. Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium. PARASITOLOGY. 2009;136(10):1161–8.
MLA
Geurden, Thomas, Bruno Levecke, SM Caccio, et al. “Multilocus Genotyping of Cryptosporidium and Giardia in Non-outbreak Related Cases of Diarrhoea in Human Patients in Belgium.” PARASITOLOGY 136.10 (2009): 1161–1168. Print.
@article{780049,
  abstract     = {Stool samples from Belgian patients Suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54.2\% and C. parvum 45.8\% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IlaA15G2R1 of C. parvum were the most prevalent, although several other subgenotypes were identified. For Giardia, sequencing at the beta-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74.4\%) in human patients. A high degree of heterogeneity was found, especially on the beta-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32.4\%) of human patients, which might have important epidemiological implications.},
  author       = {Geurden, Thomas and Levecke, Bruno and Caccio, SM and Visser, Aline and De Groote, G and Casaert, Stijn and Vercruysse, Jozef and Claerebout, Edwin},
  issn         = {0031-1820},
  journal      = {PARASITOLOGY},
  keyword      = {ZOONOTIC TRANSMISSION,DUODENALIS,MOLECULAR EPIDEMIOLOGY,risk factors,species-specific,multilocus,sporadic,prevalence,human,Giardia,Cryptosporidium,CALVES,SUBGENOTYPES,SUBTYPES,PARVUM,CHILDREN},
  language     = {eng},
  number       = {10},
  pages        = {1161--1168},
  title        = {Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium},
  url          = {http://dx.doi.org/10.1017/S0031182009990436},
  volume       = {136},
  year         = {2009},
}

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