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Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium

Thomas Geurden UGent, Bruno Levecke UGent, SM Caccio, Aline Visser UGent, G De Groote, Stijn Casaert UGent, Jozef Vercruysse UGent and Edwin Claerebout UGent (2009) PARASITOLOGY. 136(10). p.1161-1168
abstract
Stool samples from Belgian patients Suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54.2% and C. parvum 45.8% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IlaA15G2R1 of C. parvum were the most prevalent, although several other subgenotypes were identified. For Giardia, sequencing at the beta-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74.4%) in human patients. A high degree of heterogeneity was found, especially on the beta-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32.4%) of human patients, which might have important epidemiological implications.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ZOONOTIC TRANSMISSION, DUODENALIS, MOLECULAR EPIDEMIOLOGY, risk factors, species-specific, multilocus, sporadic, prevalence, human, Giardia, Cryptosporidium, CALVES, SUBGENOTYPES, SUBTYPES, PARVUM, CHILDREN
journal title
PARASITOLOGY
Parasitology
volume
136
issue
10
pages
1161 - 1168
Web of Science type
Article
Web of Science id
000269644100007
JCR category
PARASITOLOGY
JCR impact factor
1.607 (2009)
JCR rank
16/28 (2009)
JCR quartile
3 (2009)
ISSN
0031-1820
DOI
10.1017/S0031182009990436
language
English
UGent publication?
yes
classification
A1
id
780049
handle
http://hdl.handle.net/1854/LU-780049
date created
2009-11-12 14:50:06
date last changed
2009-12-01 16:46:26
@article{780049,
  abstract     = {Stool samples from Belgian patients Suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54.2\% and C. parvum 45.8\% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IlaA15G2R1 of C. parvum were the most prevalent, although several other subgenotypes were identified. For Giardia, sequencing at the beta-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74.4\%) in human patients. A high degree of heterogeneity was found, especially on the beta-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32.4\%) of human patients, which might have important epidemiological implications.},
  author       = {Geurden, Thomas and Levecke, Bruno and Caccio, SM and Visser, Aline and De Groote, G and Casaert, Stijn and Vercruysse, Jozef and Claerebout, Edwin},
  issn         = {0031-1820},
  journal      = {PARASITOLOGY},
  keyword      = {ZOONOTIC TRANSMISSION,DUODENALIS,MOLECULAR EPIDEMIOLOGY,risk factors,species-specific,multilocus,sporadic,prevalence,human,Giardia,Cryptosporidium,CALVES,SUBGENOTYPES,SUBTYPES,PARVUM,CHILDREN},
  language     = {eng},
  number       = {10},
  pages        = {1161--1168},
  title        = {Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium},
  url          = {http://dx.doi.org/10.1017/S0031182009990436},
  volume       = {136},
  year         = {2009},
}

Chicago
Geurden, Thomas, Bruno Levecke, SM Caccio, Aline Visser, G De Groote, Stijn Casaert, Jozef Vercruysse, and Edwin Claerebout. 2009. “Multilocus Genotyping of Cryptosporidium and Giardia in Non-outbreak Related Cases of Diarrhoea in Human Patients in Belgium.” Parasitology 136 (10): 1161–1168.
APA
Geurden, T., Levecke, B., Caccio, S., Visser, A., De Groote, G., Casaert, S., Vercruysse, J., et al. (2009). Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium. PARASITOLOGY, 136(10), 1161–1168.
Vancouver
1.
Geurden T, Levecke B, Caccio S, Visser A, De Groote G, Casaert S, et al. Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium. PARASITOLOGY. 2009;136(10):1161–8.
MLA
Geurden, Thomas, Bruno Levecke, SM Caccio, et al. “Multilocus Genotyping of Cryptosporidium and Giardia in Non-outbreak Related Cases of Diarrhoea in Human Patients in Belgium.” PARASITOLOGY 136.10 (2009): 1161–1168. Print.