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Tissue distribution of peroxisomes in zebrafish

Mieke Stevens UGent, Olga Krysko UGent, Myriam Baes and Marc Espeel UGent (2008) EMC 2008 : 14th European Microscopy Congress. 3 : Life Science. p.291-292
abstract
Peroxisomes are single membrane-bound cell organelles present in almost all eukaryotic cells; they are in particular abundant and large in mammalian liver and in the proximal tubules of the kidney. Peroxisomes have a fine granular matrix containing oxidases, catalase and other enzymes which are involved in VLCFA breakdown, catabolism of purines and polyamines, inactivation of toxic oxygen species and in plasmalogen synthesis [1]. The importance of these metabolic functions is emphasized by the existence of human peroxisomal disorders (Zellweger syndrome, neonatal adrenoleukodystrophy,...). In most cases patients display severe neurological dysfunctions and craniofacial malformations. Sofar, the pathogenic mechanism of these inherited metabolic disorders remains largely unexplained. In order to elucidate the pathogenesis, several mouse models have been generated [2]. In recent years zebrafish models for metabolic human diseases have been developed as an alternative for mouse models [3]. We are exploring the suitability of zebrafish as a model organism for peroxisomal biogenesis disorders. We report here our first observations on the occurrence of peroxisomes in major organs of zebrafish by DAB cytochemistry for catalase activity — catalase is the peroxisomal marker enzyme — and immunostaining against a peroxisomal membrane protein (PMP70).
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
peroxisomes, zebrafish, metabolic disorders
in
EMC 2008 : 14th European Microscopy Congress
editor
Anke Aretz, Benita Hermanns-Sachweh and Joachim Mayer
volume
3 : Life Science
pages
291 - 292
publisher
Springer
place of publication
Berlin, Germany
conference name
14th European Microscopy Congress (EMC 2008)
conference location
Aachen, Germany
conference start
2008-09-01
conference end
2008-09-05
ISBN
978-3-540-85227-8
978-3-540-85228-5
DOI
10.1007/978-3-540-85228-5_146
language
English
UGent publication?
yes
classification
C3
copyright statement
I have transferred the copyright for this publication to the publisher
id
778994
handle
http://hdl.handle.net/1854/LU-778994
date created
2009-11-09 11:36:33
date last changed
2009-12-01 11:39:42
@inproceedings{778994,
  abstract     = {Peroxisomes are single membrane-bound cell organelles present in almost all eukaryotic cells; they are in particular abundant and large in mammalian liver and in the proximal tubules of the kidney. Peroxisomes have a fine granular matrix containing oxidases, catalase and other enzymes which are involved in VLCFA breakdown, catabolism of purines and polyamines, inactivation of toxic oxygen species and in plasmalogen synthesis [1]. The importance of these metabolic functions is emphasized by the existence of human peroxisomal disorders (Zellweger syndrome, neonatal adrenoleukodystrophy,...). In most cases patients display severe neurological dysfunctions and craniofacial malformations. Sofar, the pathogenic mechanism of these inherited metabolic disorders remains largely unexplained. In order to elucidate the pathogenesis, several mouse models have been generated [2]. In recent years zebrafish models for metabolic human diseases have been developed as an alternative for mouse models [3]. We are exploring the suitability of zebrafish as a model organism for peroxisomal biogenesis disorders. We report here our first observations on the occurrence of peroxisomes in major organs of zebrafish by DAB cytochemistry for catalase activity --- catalase is the peroxisomal marker enzyme --- and immunostaining against a peroxisomal membrane protein (PMP70).},
  author       = {Stevens, Mieke and Krysko, Olga and Baes, Myriam and Espeel, Marc},
  booktitle    = {EMC 2008 : 14th European Microscopy Congress},
  editor       = {Aretz, Anke and Hermanns-Sachweh, Benita and Mayer, Joachim},
  isbn         = {978-3-540-85227-8},
  keyword      = {peroxisomes,zebrafish,metabolic disorders},
  language     = {eng},
  location     = {Aachen, Germany},
  pages        = {291--292},
  publisher    = {Springer},
  title        = {Tissue distribution of peroxisomes in zebrafish},
  url          = {http://dx.doi.org/10.1007/978-3-540-85228-5\_146},
  volume       = {3 : Life Science},
  year         = {2008},
}

Chicago
Stevens, Mieke, Olga Krysko, Myriam Baes, and Marc Espeel. 2008. “Tissue Distribution of Peroxisomes in Zebrafish.” In EMC 2008 : 14th European Microscopy Congress, ed. Anke Aretz, Benita Hermanns-Sachweh, and Joachim Mayer, 3 : Life Science:291–292. Berlin, Germany: Springer.
APA
Stevens, Mieke, Krysko, O., Baes, M., & Espeel, M. (2008). Tissue distribution of peroxisomes in zebrafish. In A. Aretz, B. Hermanns-Sachweh, & J. Mayer (Eds.), EMC 2008 : 14th European Microscopy Congress (Vol. 3 : Life Science, pp. 291–292). Presented at the 14th European Microscopy Congress (EMC 2008), Berlin, Germany: Springer.
Vancouver
1.
Stevens M, Krysko O, Baes M, Espeel M. Tissue distribution of peroxisomes in zebrafish. In: Aretz A, Hermanns-Sachweh B, Mayer J, editors. EMC 2008 : 14th European Microscopy Congress. Berlin, Germany: Springer; 2008. p. 291–2.
MLA
Stevens, Mieke, Olga Krysko, Myriam Baes, et al. “Tissue Distribution of Peroxisomes in Zebrafish.” EMC 2008 : 14th European Microscopy Congress. Ed. Anke Aretz, Benita Hermanns-Sachweh, & Joachim Mayer. 3 : Life Science. Berlin, Germany: Springer, 2008. 291–292. Print.