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Tissue distribution of peroxisomes in zebrafish

Mieke Stevens (UGent) , Olga Krysko (UGent) , Myriam Baes and Marc Espeel (UGent)
(2008) EMC 2008 : 14th European Microscopy Congress. 3 : Life Science. p.291-292
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Abstract
Peroxisomes are single membrane-bound cell organelles present in almost all eukaryotic cells; they are in particular abundant and large in mammalian liver and in the proximal tubules of the kidney. Peroxisomes have a fine granular matrix containing oxidases, catalase and other enzymes which are involved in VLCFA breakdown, catabolism of purines and polyamines, inactivation of toxic oxygen species and in plasmalogen synthesis [1]. The importance of these metabolic functions is emphasized by the existence of human peroxisomal disorders (Zellweger syndrome, neonatal adrenoleukodystrophy,...). In most cases patients display severe neurological dysfunctions and craniofacial malformations. Sofar, the pathogenic mechanism of these inherited metabolic disorders remains largely unexplained. In order to elucidate the pathogenesis, several mouse models have been generated [2]. In recent years zebrafish models for metabolic human diseases have been developed as an alternative for mouse models [3]. We are exploring the suitability of zebrafish as a model organism for peroxisomal biogenesis disorders. We report here our first observations on the occurrence of peroxisomes in major organs of zebrafish by DAB cytochemistry for catalase activity — catalase is the peroxisomal marker enzyme — and immunostaining against a peroxisomal membrane protein (PMP70).
Keywords
peroxisomes, zebrafish, metabolic disorders

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MLA
Stevens, Mieke, Olga Krysko, Myriam Baes, et al. “Tissue Distribution of Peroxisomes in Zebrafish.” EMC 2008 : 14th European Microscopy Congress. Ed. Anke Aretz, Benita Hermanns-Sachweh, & Joachim Mayer. 3 : Life Science. Berlin, Germany: Springer, 2008. 291–292. Print.
APA
Stevens, Mieke, Krysko, O., Baes, M., & Espeel, M. (2008). Tissue distribution of peroxisomes in zebrafish. In A. Aretz, B. Hermanns-Sachweh, & J. Mayer (Eds.), EMC 2008 : 14th European Microscopy Congress (Vol. 3 : Life Science, pp. 291–292). Presented at the 14th European Microscopy Congress (EMC 2008), Berlin, Germany: Springer.
Chicago author-date
Stevens, Mieke, Olga Krysko, Myriam Baes, and Marc Espeel. 2008. “Tissue Distribution of Peroxisomes in Zebrafish.” In EMC 2008 : 14th European Microscopy Congress, ed. Anke Aretz, Benita Hermanns-Sachweh, and Joachim Mayer, 3 : Life Science:291–292. Berlin, Germany: Springer.
Chicago author-date (all authors)
Stevens, Mieke, Olga Krysko, Myriam Baes, and Marc Espeel. 2008. “Tissue Distribution of Peroxisomes in Zebrafish.” In EMC 2008 : 14th European Microscopy Congress, ed. Anke Aretz, Benita Hermanns-Sachweh, and Joachim Mayer, 3 : Life Science:291–292. Berlin, Germany: Springer.
Vancouver
1.
Stevens M, Krysko O, Baes M, Espeel M. Tissue distribution of peroxisomes in zebrafish. In: Aretz A, Hermanns-Sachweh B, Mayer J, editors. EMC 2008 : 14th European Microscopy Congress. Berlin, Germany: Springer; 2008. p. 291–2.
IEEE
[1]
M. Stevens, O. Krysko, M. Baes, and M. Espeel, “Tissue distribution of peroxisomes in zebrafish,” in EMC 2008 : 14th European Microscopy Congress, Aachen, Germany, 2008, vol. 3 : Life Science, pp. 291–292.
@inproceedings{778994,
  abstract     = {Peroxisomes are single membrane-bound cell organelles present in almost all eukaryotic cells; they are in particular abundant and large in mammalian liver and in the proximal tubules of the kidney. Peroxisomes have a fine granular matrix containing oxidases, catalase and other enzymes which are involved in VLCFA breakdown, catabolism of purines and polyamines, inactivation of toxic oxygen species and in plasmalogen synthesis [1]. The importance of these metabolic functions is emphasized by the existence of human peroxisomal disorders (Zellweger syndrome, neonatal adrenoleukodystrophy,...). In most cases patients display severe neurological dysfunctions and craniofacial malformations. Sofar, the pathogenic mechanism of these inherited metabolic disorders remains largely unexplained. In order to elucidate the pathogenesis, several mouse models have been generated [2]. In recent years zebrafish models for metabolic human diseases have been developed as an alternative for mouse models [3]. We are exploring the suitability of zebrafish as a model organism for peroxisomal biogenesis disorders. We report here our first observations on the occurrence of peroxisomes in major organs of zebrafish by DAB cytochemistry for catalase activity — catalase is the peroxisomal marker enzyme — and immunostaining against a peroxisomal membrane protein (PMP70).},
  author       = {Stevens, Mieke and Krysko, Olga and Baes, Myriam and Espeel, Marc},
  booktitle    = {EMC 2008 : 14th European Microscopy Congress},
  editor       = {Aretz, Anke and Hermanns-Sachweh, Benita and Mayer, Joachim},
  isbn         = {978-3-540-85227-8},
  keywords     = {peroxisomes,zebrafish,metabolic disorders},
  language     = {eng},
  location     = {Aachen, Germany},
  pages        = {291--292},
  publisher    = {Springer},
  title        = {Tissue distribution of peroxisomes in zebrafish},
  url          = {http://dx.doi.org/10.1007/978-3-540-85228-5_146},
  volume       = {3 : Life Science},
  year         = {2008},
}

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