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Expression and putative function of fibronectin and its receptor (integrin alpha-5 beta-1) in male and female gametes during bovine fertilization in vitro

Mirjan Thys UGent, Hans Nauwynck UGent, Dominiek Maes UGent, Maarten Hoogewijs UGent, Dries Vercauteren UGent, Tom Rijsselaere UGent, Herman Favoreel UGent and Ann Van Soom UGent (2009) REPRODUCTION. 138(3). p.471-482
abstract
Fibronectin (Fn) is a 440 kDa glycoprotein assumed to participate in sperm-egg interaction in human. Recently, it has been demonstrated that Fn - when present during bovine IVF - strongly inhibits sperm penetration. The present study was conducted firstly to evaluate the expression of Fn and its integrin receptor (alpha(5)beta(1)) on male and female bovine gametes using indirect immunofluorescence and secondly, to determine the function of Fn during bovine IVF. Endogenous Fn was detected underneath the zona pellucida (ZP) and integrin alpha(5) on the oolemma of cumulus-denuded oocytes. Bovine spermatozoa displayed integrin alpha(5) at their equatorial segment after acrosome reaction. We established that the main inhibitory effect of exogenously supplemented Fn was located at the sperm-oolemma binding, with a (concurrent) effect on fusion, and this can probably be attributed to the binding of Fin to spermatozoa at the equatorial segment, as shown by means of Alexa Fluor 488-conjugated Fn. Combining these results, the inhibitory effect of exogenously supplemented Fn seemed to be exerted on the male gamete by binding to the exposed integrin alpha(5)beta(1), receptor after acrosome reaction. The presence of endogenous Fn underneath the ZP together with integrin alpha(5) expression on oolemma and acrosome-reacted (AR) sperm cell surface suggests a 'velcro' interaction between the endogenous Fn ligand and corresponding receptors on both (AR) sperm cell and oolemma, initiating sperm-egg binding. Reproduction (2009) 138 471-482
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARG-GLY-ASP, SPERM SURFACE-PROTEINS, OVIDUCTAL EPITHELIUM, ZONA-PELLUCIDA, MOLECULAR-MECHANISMS, ACROSOME REACTION, OOCYTE FUSION, BINDING, CAPACITATION, RECOGNITION
journal title
REPRODUCTION
Reproduction
volume
138
issue
3
pages
471 - 482
Web of Science type
Article
Web of Science id
000270101100006
JCR category
REPRODUCTIVE BIOLOGY
JCR impact factor
2.579 (2009)
JCR rank
9/26 (2009)
JCR quartile
2 (2009)
ISSN
1470-1626
DOI
10.1530/REP-09-0094
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
768513
handle
http://hdl.handle.net/1854/LU-768513
date created
2009-10-26 13:54:17
date last changed
2009-11-26 11:58:12
@article{768513,
  abstract     = {Fibronectin (Fn) is a 440 kDa glycoprotein assumed to participate in sperm-egg interaction in human. Recently, it has been demonstrated that Fn - when present during bovine IVF - strongly inhibits sperm penetration. The present study was conducted firstly to evaluate the expression of Fn and its integrin receptor (alpha(5)beta(1)) on male and female bovine gametes using indirect immunofluorescence and secondly, to determine the function of Fn during bovine IVF. Endogenous Fn was detected underneath the zona pellucida (ZP) and integrin alpha(5) on the oolemma of cumulus-denuded oocytes. Bovine spermatozoa displayed integrin alpha(5) at their equatorial segment after acrosome reaction. We established that the main inhibitory effect of exogenously supplemented Fn was located at the sperm-oolemma binding, with a (concurrent) effect on fusion, and this can probably be attributed to the binding of Fin to spermatozoa at the equatorial segment, as shown by means of Alexa Fluor 488-conjugated Fn. Combining these results, the inhibitory effect of exogenously supplemented Fn seemed to be exerted on the male gamete by binding to the exposed integrin alpha(5)beta(1), receptor after acrosome reaction. The presence of endogenous Fn underneath the ZP together with integrin alpha(5) expression on oolemma and acrosome-reacted (AR) sperm cell surface suggests a 'velcro' interaction between the endogenous Fn ligand and corresponding receptors on both (AR) sperm cell and oolemma, initiating sperm-egg binding. Reproduction (2009) 138 471-482},
  author       = {Thys, Mirjan and Nauwynck, Hans and Maes, Dominiek and Hoogewijs, Maarten and Vercauteren, Dries and Rijsselaere, Tom and Favoreel, Herman and Van Soom, Ann},
  issn         = {1470-1626},
  journal      = {REPRODUCTION},
  keyword      = {ARG-GLY-ASP,SPERM SURFACE-PROTEINS,OVIDUCTAL EPITHELIUM,ZONA-PELLUCIDA,MOLECULAR-MECHANISMS,ACROSOME REACTION,OOCYTE FUSION,BINDING,CAPACITATION,RECOGNITION},
  language     = {eng},
  number       = {3},
  pages        = {471--482},
  title        = {Expression and putative function of fibronectin and its receptor (integrin alpha-5 beta-1) in male and female gametes during bovine fertilization in vitro},
  url          = {http://dx.doi.org/10.1530/REP-09-0094},
  volume       = {138},
  year         = {2009},
}

Chicago
Thys, Mirjan, Hans Nauwynck, Dominiek Maes, Maarten Hoogewijs, Dries Vercauteren, Tom Rijsselaere, Herman Favoreel, and Ann Van Soom. 2009. “Expression and Putative Function of Fibronectin and Its Receptor (integrin Alpha-5 Beta-1) in Male and Female Gametes During Bovine Fertilization in Vitro.” Reproduction 138 (3): 471–482.
APA
Thys, M., Nauwynck, H., Maes, D., Hoogewijs, M., Vercauteren, D., Rijsselaere, T., Favoreel, H., et al. (2009). Expression and putative function of fibronectin and its receptor (integrin alpha-5 beta-1) in male and female gametes during bovine fertilization in vitro. REPRODUCTION, 138(3), 471–482.
Vancouver
1.
Thys M, Nauwynck H, Maes D, Hoogewijs M, Vercauteren D, Rijsselaere T, et al. Expression and putative function of fibronectin and its receptor (integrin alpha-5 beta-1) in male and female gametes during bovine fertilization in vitro. REPRODUCTION. 2009;138(3):471–82.
MLA
Thys, Mirjan, Hans Nauwynck, Dominiek Maes, et al. “Expression and Putative Function of Fibronectin and Its Receptor (integrin Alpha-5 Beta-1) in Male and Female Gametes During Bovine Fertilization in Vitro.” REPRODUCTION 138.3 (2009): 471–482. Print.