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Establishing a model organism for stem cell research: the stem cell system of the rhabditophoran flatworm Macrostomum lignano during embryonic development, post-embryonic development, regeneration and ageing

Maxime Willems UGent (2008)
abstract
The model system approach is an extraordinary powerful tool to study animal development or any other biological process. The challenge is to determine the best criteria for choosing new model organisms, given limited resources. Examples of such criteria are that (1) the selected animal should be one in which the trait of interest (e.g. stem cells, regeneration) is patently manifested, (2) the selected animal should be relatively easy to manipulate experimentally and (3) co-option of techniques and resources that were developed for established models should be possible. How flatworms in general and Macrostomum lignano in particular fulfil these criteria is shown in this thesis. Flatworms (Platyhelminthes) are a group of relatively simple, soft-bodied invertebrate animals. We choose to work on the macrostomid M. lignano for numerous reasons (e.g. ease of culture, relatively transparent habitus, short lifespan, quantifiable organ systems and stem cell system, monoclonal antibodies). The central theme of this research project is the neoblast stem cell system. Neoblast are responsible for growth, homeostasis and regeneration, yet many aspects of their biology are still poorly understood (embryonic origin, dynamics in homeostasis, germ line formation). Therefore, we conducted a study to characterize the stem cell system during many phases of the life history of M. lignano. The major goals in this project are: 1. establishing a new model organism in our laboratory: this was one of the most challenging and time-consuming aspects of this project; 2. studying the embryonic (and post-embryonic) development of M. lignano, ultimately with a focus on the neoblast system; 3. analyzing the stem cell dynamics during regeneration in M. lignano; 4. explore the potential of M. lignano as a model for ageing research, especially to study the relation between stem cells and ageing.
Please use this url to cite or link to this publication:
author
promoter
UGent and Tom Artois
organization
year
type
dissertation (monograph)
subject
pages
332 pages
publisher
Ghent University. Faculty of Sciences
place of publication
Ghent, Belgium
defense location
Gent : Het Pand (zaal rector Vermeylen)
defense date
2008-12-12 17:00
language
English
UGent publication?
yes
classification
D1
additional info
dissertation consists of copyrighted materials
copyright statement
I have transferred the copyright for this publication to the publisher
id
763027
handle
http://hdl.handle.net/1854/LU-763027
alternative location
http://lib.ugent.be/fulltxt/RUG01/001/305/682/RUG01-001305682_2010_0001_AC.pdf
date created
2009-10-14 15:11:13
date last changed
2010-11-24 09:55:46
@phdthesis{763027,
  abstract     = {The model system approach is an extraordinary powerful tool to study animal development or any other biological process. The challenge is to determine the best criteria for choosing new model organisms, given limited resources. Examples of such criteria are that (1) the selected animal should be one in which the trait of interest (e.g. stem cells, regeneration) is patently manifested, (2) the selected animal should be relatively easy to manipulate experimentally and (3) co-option of techniques and resources that were developed for established models should be possible. How flatworms in general and Macrostomum lignano in particular fulfil these criteria is shown in this thesis. Flatworms (Platyhelminthes) are a group of relatively simple, soft-bodied invertebrate animals. We choose to work on the macrostomid M. lignano for numerous reasons (e.g. ease of culture, relatively transparent habitus, short lifespan, quantifiable organ systems and stem cell system, monoclonal antibodies).
The central theme of this research project is the neoblast stem cell system. Neoblast are responsible for growth, homeostasis and regeneration, yet many aspects of their biology are still poorly understood (embryonic origin, dynamics in homeostasis, germ line formation). Therefore, we conducted a study to characterize the stem cell system during many phases of the life history of M. lignano.
The major goals in this project are:
1. establishing a new model organism in our laboratory: this was one of the most challenging and time-consuming aspects of this project;
2. studying the embryonic (and post-embryonic) development of M. lignano, ultimately with a focus on the neoblast system;
3. analyzing the stem cell dynamics during regeneration in M. lignano;
4. explore the potential of M. lignano as a model for ageing research, especially to study the relation between stem cells and ageing.},
  author       = {Willems, Maxime},
  language     = {eng},
  pages        = {332},
  publisher    = {Ghent University. Faculty of Sciences},
  school       = {Ghent University},
  title        = {Establishing a model organism for stem cell research: the stem cell system of the rhabditophoran flatworm Macrostomum lignano during embryonic development, post-embryonic development, regeneration and ageing},
  url          = {http://lib.ugent.be/fulltxt/RUG01/001/305/682/RUG01-001305682\_2010\_0001\_AC.pdf},
  year         = {2008},
}

Chicago
Willems, Maxime. 2008. “Establishing a Model Organism for Stem Cell Research: The Stem Cell System of the Rhabditophoran Flatworm Macrostomum Lignano During Embryonic Development, Post-embryonic Development, Regeneration and Ageing”. Ghent, Belgium: Ghent University. Faculty of Sciences.
APA
Willems, M. (2008). Establishing a model organism for stem cell research: the stem cell system of the rhabditophoran flatworm Macrostomum lignano during embryonic development, post-embryonic development, regeneration and ageing. Ghent University. Faculty of Sciences, Ghent, Belgium.
Vancouver
1.
Willems M. Establishing a model organism for stem cell research: the stem cell system of the rhabditophoran flatworm Macrostomum lignano during embryonic development, post-embryonic development, regeneration and ageing. [Ghent, Belgium]: Ghent University. Faculty of Sciences; 2008.
MLA
Willems, Maxime. “Establishing a Model Organism for Stem Cell Research: The Stem Cell System of the Rhabditophoran Flatworm Macrostomum Lignano During Embryonic Development, Post-embryonic Development, Regeneration and Ageing.” 2008 : n. pag. Print.