Advanced search
1 file | 434.45 KB Add to list

Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem

(2009) FEMS MICROBIOLOGY ECOLOGY. 69(2). p.231-242
Author
Organization
Abstract
In this study, the prebiotic potential of arabinoxylan oligosaccharides (AXOS) was compared with inulin in two simulators of the human intestinal microbial ecosystem. Microbial breakdown of both oligosaccharides and short-chain fatty acid production was colon compartment specific, with ascending and transverse colon being the predominant site of inulin and AXOS degradation, respectively. Lactate levels (+5.5 mM) increased in the ascending colon during AXOS supplementation, while propionate levels (+5.1 mM) increased in the transverse colon. The concomitant decrease in lactate in the transverse colon suggests that propionate was partially formed over the acrylate pathway. Furthermore, AXOS supplementation strongly decreased butyrate in the ascending colon, this in parallel with a decrease in Roseburia spp. and Bacteroides/Prevotella/Porphyromonas (-1.4 and -2.0 log CFU) levels. Inulin treatment had moderate effects on lactate, propionate and butyrate levels. Denaturing gradient gel electrophoresis analysis revealed that inulin changed microbial metabolism by modulating the microbial community composition. In contrast, AXOS primarily affected microbial metabolism by 'switching on' AXOS-degrading enzymes (xylanase, arabinofuranosidase and xylosidase), without significantly affecting microbial community composition. Our results demonstrate that AXOS has a higher potency than inulin to shift part of the sugar fermentation toward the distal colon parts. Furthermore, due to its stronger propionate-stimulating effect, AXOS is a candidate prebiotic capable of lowering cholesterol and beneficially affecting fat metabolism of the host.
Keywords
inulin, arabinoxylan oligosaccharides, prebiotic, SHIME, Roseburia, Faecalibacterium prausnitzii, GRADIENT GEL-ELECTROPHORESIS, 8-PRENYLNARINGENIN IN-VITRO, HUMULUS-LUPULUS L., BIFIDOBACTERIUM-ADOLESCENTIS, HEALTHY HUMANS, GUT MICROBIOTA, HYDROLASE ACTIVITY, WHEAT BRAN, LIPID-METABOLISM, HUMAN FECES

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 434.45 KB

Citation

Please use this url to cite or link to this publication:

MLA
Grootaert, Charlotte et al. “Comparison of Prebiotic Effects of Arabinoxylan Oligosaccharides and Inulin in a Simulator of the Human Intestinal Microbial Ecosystem.” FEMS MICROBIOLOGY ECOLOGY 69.2 (2009): 231–242. Print.
APA
Grootaert, C., Van den Abbeele, P., Marzorati, M., Broeckaert, W. F., Courtin, C. M., Delcour, J. A., Verstraete, W., et al. (2009). Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS MICROBIOLOGY ECOLOGY, 69(2), 231–242.
Chicago author-date
Grootaert, Charlotte, Pieter Van den Abbeele, Massimo Marzorati, Willem F Broeckaert, Christophe M Courtin, Jan A Delcour, Willy Verstraete, and Tom Van de Wiele. 2009. “Comparison of Prebiotic Effects of Arabinoxylan Oligosaccharides and Inulin in a Simulator of the Human Intestinal Microbial Ecosystem.” Fems Microbiology Ecology 69 (2): 231–242.
Chicago author-date (all authors)
Grootaert, Charlotte, Pieter Van den Abbeele, Massimo Marzorati, Willem F Broeckaert, Christophe M Courtin, Jan A Delcour, Willy Verstraete, and Tom Van de Wiele. 2009. “Comparison of Prebiotic Effects of Arabinoxylan Oligosaccharides and Inulin in a Simulator of the Human Intestinal Microbial Ecosystem.” Fems Microbiology Ecology 69 (2): 231–242.
Vancouver
1.
Grootaert C, Van den Abbeele P, Marzorati M, Broeckaert WF, Courtin CM, Delcour JA, et al. Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS MICROBIOLOGY ECOLOGY. 2009;69(2):231–42.
IEEE
[1]
C. Grootaert et al., “Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem,” FEMS MICROBIOLOGY ECOLOGY, vol. 69, no. 2, pp. 231–242, 2009.
@article{751706,
  abstract     = {In this study, the prebiotic potential of arabinoxylan oligosaccharides (AXOS) was compared with inulin in two simulators of the human intestinal microbial ecosystem. Microbial breakdown of both oligosaccharides and short-chain fatty acid production was colon compartment specific, with ascending and transverse colon being the predominant site of inulin and AXOS degradation, respectively. Lactate levels (+5.5 mM) increased in the ascending colon during AXOS supplementation, while propionate levels (+5.1 mM) increased in the transverse colon. The concomitant decrease in lactate in the transverse colon suggests that propionate was partially formed over the acrylate pathway. Furthermore, AXOS supplementation strongly decreased butyrate in the ascending colon, this in parallel with a decrease in Roseburia spp. and Bacteroides/Prevotella/Porphyromonas (-1.4 and -2.0 log CFU) levels. Inulin treatment had moderate effects on lactate, propionate and butyrate levels. Denaturing gradient gel electrophoresis analysis revealed that inulin changed microbial metabolism by modulating the microbial community composition. In contrast, AXOS primarily affected microbial metabolism by 'switching on' AXOS-degrading enzymes (xylanase, arabinofuranosidase and xylosidase), without significantly affecting microbial community composition. Our results demonstrate that AXOS has a higher potency than inulin to shift part of the sugar fermentation toward the distal colon parts. Furthermore, due to its stronger propionate-stimulating effect, AXOS is a candidate prebiotic capable of lowering cholesterol and beneficially affecting fat metabolism of the host.},
  author       = {Grootaert, Charlotte and Van den Abbeele, Pieter and Marzorati, Massimo and Broeckaert, Willem F and Courtin, Christophe M and Delcour, Jan A and Verstraete, Willy and Van de Wiele, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keywords     = {inulin,arabinoxylan oligosaccharides,prebiotic,SHIME,Roseburia,Faecalibacterium prausnitzii,GRADIENT GEL-ELECTROPHORESIS,8-PRENYLNARINGENIN IN-VITRO,HUMULUS-LUPULUS L.,BIFIDOBACTERIUM-ADOLESCENTIS,HEALTHY HUMANS,GUT MICROBIOTA,HYDROLASE ACTIVITY,WHEAT BRAN,LIPID-METABOLISM,HUMAN FECES},
  language     = {eng},
  number       = {2},
  pages        = {231--242},
  title        = {Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2009.00712.x},
  volume       = {69},
  year         = {2009},
}

Altmetric
View in Altmetric
Web of Science
Times cited: