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Recombination of two porcine circovirus type 2 strains

(2009) ARCHIVES OF VIROLOGY. 154(5). p.875-879
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Abstract
Pigs can be concurrently infected with different PCV2 strains. In this study, a cell-culture-adapted PCV2 strain, originating from a PMWS-affected pig, was purified by limiting dilution. Three different strains were obtained, and one of them was a perfect mosaic of the other two, with recombination breakpoints in ORF1 and ORF2. Incongruence was observed between phylogenetic trees constructed with the whole genome, ORF1 and ORF2. Amplification of ORF1 and ORF2 from original material, followed by cloning and sequencing, resulted in sequences corresponding to the parental strains, but not with the mosaic strain. These results demonstrate that PCV2 can undergo recombination.
Keywords
SYNDROME PMWS, MULTISYSTEMIC WASTING-SYNDROME, PIGS, VIRUS, PORCINE-CIRCOVIRUS-TYPE-2, SWINE, DISEASE, NUCLEOTIDE-SEQUENCE, GENOTYPE DEFINITION

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Citation

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MLA
Lefebvre, David, et al. “Recombination of Two Porcine Circovirus Type 2 Strains.” ARCHIVES OF VIROLOGY, vol. 154, no. 5, 2009, pp. 875–79, doi:10.1007/s00705-009-0379-8.
APA
Lefebvre, D., Van Doorsselaere, J., Delputte, P., & Nauwynck, H. (2009). Recombination of two porcine circovirus type 2 strains. ARCHIVES OF VIROLOGY, 154(5), 875–879. https://doi.org/10.1007/s00705-009-0379-8
Chicago author-date
Lefebvre, David, J Van Doorsselaere, Peter Delputte, and Hans Nauwynck. 2009. “Recombination of Two Porcine Circovirus Type 2 Strains.” ARCHIVES OF VIROLOGY 154 (5): 875–79. https://doi.org/10.1007/s00705-009-0379-8.
Chicago author-date (all authors)
Lefebvre, David, J Van Doorsselaere, Peter Delputte, and Hans Nauwynck. 2009. “Recombination of Two Porcine Circovirus Type 2 Strains.” ARCHIVES OF VIROLOGY 154 (5): 875–879. doi:10.1007/s00705-009-0379-8.
Vancouver
1.
Lefebvre D, Van Doorsselaere J, Delputte P, Nauwynck H. Recombination of two porcine circovirus type 2 strains. ARCHIVES OF VIROLOGY. 2009;154(5):875–9.
IEEE
[1]
D. Lefebvre, J. Van Doorsselaere, P. Delputte, and H. Nauwynck, “Recombination of two porcine circovirus type 2 strains,” ARCHIVES OF VIROLOGY, vol. 154, no. 5, pp. 875–879, 2009.
@article{749195,
  abstract     = {{Pigs can be concurrently infected with different PCV2 strains. In this study, a cell-culture-adapted PCV2 strain, originating from a PMWS-affected pig, was purified by limiting dilution. Three different strains were obtained, and one of them was a perfect mosaic of the other two, with recombination breakpoints in ORF1 and ORF2. Incongruence was observed between phylogenetic trees constructed with the whole genome, ORF1 and ORF2. Amplification of ORF1 and ORF2 from original material, followed by cloning and sequencing, resulted in sequences corresponding to the parental strains, but not with the mosaic strain. These results demonstrate that PCV2 can undergo recombination.}},
  author       = {{Lefebvre, David and Van Doorsselaere, J and Delputte, Peter and Nauwynck, Hans}},
  issn         = {{0304-8608}},
  journal      = {{ARCHIVES OF VIROLOGY}},
  keywords     = {{SYNDROME PMWS,MULTISYSTEMIC WASTING-SYNDROME,PIGS,VIRUS,PORCINE-CIRCOVIRUS-TYPE-2,SWINE,DISEASE,NUCLEOTIDE-SEQUENCE,GENOTYPE DEFINITION}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{875--879}},
  title        = {{Recombination of two porcine circovirus type 2 strains}},
  url          = {{http://doi.org/10.1007/s00705-009-0379-8}},
  volume       = {{154}},
  year         = {{2009}},
}

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