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Evaluation of different scan methods for the urinary detection of corticosteroid metabolites by liquid chromatography tandem mass spectrometry

(2009) JOURNAL OF MASS SPECTROMETRY. 44(6). p.929-944
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Abstract
Different approaches for the non-target detection of corticosteroids in urine have been evaluated. As a result of previous studies about the ionization (positive/negative) and fragmentation of corticosteroids, several methods based on both precursor ion (PI) and neutral loss (NL) scans are proposed. The applicability of these methods was checked by the injection of a standard solution containing 19 model compounds. Five of the studied methods (NL of 76 Da; PI of 77, 91 and 105; PI of 237; PI of 121,147 and 171; and NIL of 38 Da) exhibited satisfactory results at the concentration level checked (corresponding to 20 ng/ml in sample). Some other methods in negative ionization mode such as the NIL of 104 Da were found to lack sufficient sensitivity. Some of the applied methods were found to be specific for a concrete structure (NL of 38 Da for fluorine containing corticosteroids) while others showed a wide range applicability (PI of 77, 91 and 105 showed response in all model compounds). interference by endogenous compounds was also tested by the analysis of negative urines and urines spiked with different corticosteroids. The suitability of these methods for the detection of corticosteroid metabolites was checked by the analysis of urine samples collected after the administration of methylprednisolone and triamcinolone. A combination of the reported methods seems to be the approach of choice in order to have a global overview about the excreted corticosteroid metabolites.
Keywords
doping analysis, corticosteroids, metabolites, precursor ion scan, neutral loss, unknown compounds, COLLISION-INDUCED DISSOCIATION, ANABOLIC-STEROIDS, DOPING ANALYSIS, SCREENING METHOD, LC-MS, ELUCIDATION, BUDESONIDE, DRUGS, IONIZATION

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Chicago
Pozo Mendoza, Oscar Juan, R Ventura, N Monfort, J Segura, and Frans Delbeke. 2009. “Evaluation of Different Scan Methods for the Urinary Detection of Corticosteroid Metabolites by Liquid Chromatography Tandem Mass Spectrometry.” Journal of Mass Spectrometry 44 (6): 929–944.
APA
Pozo Mendoza, O. J., Ventura, R., Monfort, N., Segura, J., & Delbeke, F. (2009). Evaluation of different scan methods for the urinary detection of corticosteroid metabolites by liquid chromatography tandem mass spectrometry. JOURNAL OF MASS SPECTROMETRY, 44(6), 929–944.
Vancouver
1.
Pozo Mendoza OJ, Ventura R, Monfort N, Segura J, Delbeke F. Evaluation of different scan methods for the urinary detection of corticosteroid metabolites by liquid chromatography tandem mass spectrometry. JOURNAL OF MASS SPECTROMETRY. 2009;44(6):929–44.
MLA
Pozo Mendoza, Oscar Juan, R Ventura, N Monfort, et al. “Evaluation of Different Scan Methods for the Urinary Detection of Corticosteroid Metabolites by Liquid Chromatography Tandem Mass Spectrometry.” JOURNAL OF MASS SPECTROMETRY 44.6 (2009): 929–944. Print.
@article{749097,
  abstract     = {Different approaches for the non-target detection of corticosteroids in urine have been evaluated. As a result of previous studies about the ionization (positive/negative) and fragmentation of corticosteroids, several methods based on both precursor ion (PI) and neutral loss (NL) scans are proposed. The applicability of these methods was checked by the injection of a standard solution containing 19 model compounds. Five of the studied methods (NL of 76 Da; PI of 77, 91 and 105; PI of 237; PI of 121,147 and 171; and NIL of 38 Da) exhibited satisfactory results at the concentration level checked (corresponding to 20 ng/ml in sample). Some other methods in negative ionization mode such as the NIL of 104 Da were found to lack sufficient sensitivity. Some of the applied methods were found to be specific for a concrete structure (NL of 38 Da for fluorine containing corticosteroids) while others showed a wide range applicability (PI of 77, 91 and 105 showed response in all model compounds). interference by endogenous compounds was also tested by the analysis of negative urines and urines spiked with different corticosteroids. The suitability of these methods for the detection of corticosteroid metabolites was checked by the analysis of urine samples collected after the administration of methylprednisolone and triamcinolone. A combination of the reported methods seems to be the approach of choice in order to have a global overview about the excreted corticosteroid metabolites.},
  author       = {Pozo Mendoza, Oscar Juan and Ventura, R and Monfort, N and Segura, J and Delbeke, Frans},
  issn         = {1076-5174},
  journal      = {JOURNAL OF MASS SPECTROMETRY},
  language     = {eng},
  number       = {6},
  pages        = {929--944},
  title        = {Evaluation of different scan methods for the urinary detection of corticosteroid metabolites by liquid chromatography tandem mass spectrometry},
  url          = {http://dx.doi.org/10.1002/jms.1568},
  volume       = {44},
  year         = {2009},
}

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