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Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size

Cristian Antonio Rojas, Nubia Barbosa Eloy UGent, Marcelo de Freitas Lima, Roberta Lopes Rodrigues, Luciana Ozório Franco, Kristiina Himanen UGent, Gerrit Beemster UGent, Adriana S Hemerly UGent and Paulo Cavalcanti Gomes Ferreira (2009) PLANT MOLECULAR BIOLOGY. 71(3). p.307-318
abstract
The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher H-3-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DIFFERENTIATION, THALIANA, TRANSFORMATION, PROLIFERATION, REPLICATION, DIVISION, GENE-EXPRESSION, TRANSCRIPTION FACTOR, PLANT-CELL CYCLE, CYCLIN-DEPENDENT KINASES, Arabidopsis, Development, APC, Cell cycle
journal title
PLANT MOLECULAR BIOLOGY
Plant Mol. Biol.
volume
71
issue
3
pages
307 - 318
Web of Science type
Article
Web of Science id
000269152200008
JCR category
PLANT SCIENCES
JCR impact factor
3.978 (2009)
JCR rank
15/172 (2009)
JCR quartile
1 (2009)
ISSN
0167-4412
DOI
10.1007/s11103-009-9525-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
749058
handle
http://hdl.handle.net/1854/LU-749058
date created
2009-09-15 12:15:08
date last changed
2013-10-17 11:12:40
@article{749058,
  abstract     = {The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher H-3-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.},
  author       = {Rojas, Cristian Antonio and Eloy, Nubia Barbosa and de Freitas Lima, Marcelo and Lopes Rodrigues, Roberta and Oz{\'o}rio Franco, Luciana and Himanen, Kristiina and Beemster, Gerrit and Hemerly, Adriana S and Cavalcanti Gomes Ferreira, Paulo},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keyword      = {DIFFERENTIATION,THALIANA,TRANSFORMATION,PROLIFERATION,REPLICATION,DIVISION,GENE-EXPRESSION,TRANSCRIPTION FACTOR,PLANT-CELL CYCLE,CYCLIN-DEPENDENT KINASES,Arabidopsis,Development,APC,Cell cycle},
  language     = {eng},
  number       = {3},
  pages        = {307--318},
  title        = {Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size},
  url          = {http://dx.doi.org/10.1007/s11103-009-9525-7},
  volume       = {71},
  year         = {2009},
}

Chicago
Rojas, Cristian Antonio, Nubia Barbosa Eloy, Marcelo de Freitas Lima, Roberta Lopes Rodrigues, Luciana Ozório Franco, Kristiina Himanen, Gerrit Beemster, Adriana S Hemerly, and Paulo Cavalcanti Gomes Ferreira. 2009. “Overexpression of the Arabidopsis Anaphase Promoting Complex Subunit CDC27a Increases Growth Rate and Organ Size.” Plant Molecular Biology 71 (3): 307–318.
APA
Rojas, C. A., Eloy, N. B., de Freitas Lima, M., Lopes Rodrigues, R., Ozório Franco, L., Himanen, K., Beemster, G., et al. (2009). Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size. PLANT MOLECULAR BIOLOGY, 71(3), 307–318.
Vancouver
1.
Rojas CA, Eloy NB, de Freitas Lima M, Lopes Rodrigues R, Ozório Franco L, Himanen K, et al. Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size. PLANT MOLECULAR BIOLOGY. 2009;71(3):307–18.
MLA
Rojas, Cristian Antonio, Nubia Barbosa Eloy, Marcelo de Freitas Lima, et al. “Overexpression of the Arabidopsis Anaphase Promoting Complex Subunit CDC27a Increases Growth Rate and Organ Size.” PLANT MOLECULAR BIOLOGY 71.3 (2009): 307–318. Print.