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Quantification of testosterone undecanoate in human hair by liquid chromatography-tandem mass spectrometry

Oscar Juan Pozo Mendoza UGent, Koen Deventer UGent, Peter Van Eenoo UGent, Robert Rubens UGent and Frans Delbeke UGent (2009) BIOMEDICAL CHROMATOGRAPHY. 23(8). p.873-880
abstract
Testosterone undecanoate (T-C11) can be used by athletes in order to improve performance. After oral intake, T-Cl I is rapidly metabolized, hampering discrimination between exogenous and endogenous testosterone. A possible alternative is to detect the intact ester in hair. A method based on liquid chromatography-tandem mass spectrometry was developed for the determination of T-C11 in hair. The sample procedure consisted of digestion of 200 mg of pulverized hair with tris(2-carboxyethyl)phosphine hydrochloride and liquid-liquid extraction with n-pentane. Several parameters such as the mobile phase, the ionization source and the washing step were optimized. The method was validated at different spiked levels obtaining satisfactory values for accuracy (between 92 and 102%) with relative standard deviations lower than 7% and a limit of detection of 0.2 ng/g. The applicability of the method was checked by the analysis of three samples from patients using T-C11. A peak for the analyte was detected in all samples with concentrations between 0.4 and 8.4 ng/g.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
quantification, anabolic steroids, doping analysis, hair, mass spectrometry, COLLISION-INDUCED DISSOCIATION, ANABOLIC-STEROIDS, DOPING CONTROL, SCREENING METHOD, ESTERS, IDENTIFICATION, MS, URINE, LINE
journal title
BIOMEDICAL CHROMATOGRAPHY
Biomed. Chromatogr.
volume
23
issue
8
pages
873 - 880
Web of Science type
Article
Web of Science id
000268286900012
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
1.639 (2009)
JCR rank
39/70 (2009)
JCR quartile
3 (2009)
ISSN
0269-3879
DOI
10.1002/bmc.1199
language
English
UGent publication?
yes
classification
A1
id
748970
handle
http://hdl.handle.net/1854/LU-748970
date created
2009-09-15 11:49:49
date last changed
2009-10-01 14:34:28
@article{748970,
  abstract     = {Testosterone undecanoate (T-C11) can be used by athletes in order to improve performance. After oral intake, T-Cl I is rapidly metabolized, hampering discrimination between exogenous and endogenous testosterone. A possible alternative is to detect the intact ester in hair. A method based on liquid chromatography-tandem mass spectrometry was developed for the determination of T-C11 in hair. The sample procedure consisted of digestion of 200 mg of pulverized hair with tris(2-carboxyethyl)phosphine hydrochloride and liquid-liquid extraction with n-pentane. Several parameters such as the mobile phase, the ionization source and the washing step were optimized. The method was validated at different spiked levels obtaining satisfactory values for accuracy (between 92 and 102\%) with relative standard deviations lower than 7\% and a limit of detection of 0.2 ng/g. The applicability of the method was checked by the analysis of three samples from patients using T-C11. A peak for the analyte was detected in all samples with concentrations between 0.4 and 8.4 ng/g.},
  author       = {Pozo Mendoza, Oscar Juan and Deventer, Koen and Van Eenoo, Peter and Rubens, Robert and Delbeke, Frans},
  issn         = {0269-3879},
  journal      = {BIOMEDICAL CHROMATOGRAPHY},
  keyword      = {quantification,anabolic steroids,doping analysis,hair,mass spectrometry,COLLISION-INDUCED DISSOCIATION,ANABOLIC-STEROIDS,DOPING CONTROL,SCREENING METHOD,ESTERS,IDENTIFICATION,MS,URINE,LINE},
  language     = {eng},
  number       = {8},
  pages        = {873--880},
  title        = {Quantification of testosterone undecanoate in human hair by liquid chromatography-tandem mass spectrometry},
  url          = {http://dx.doi.org/10.1002/bmc.1199},
  volume       = {23},
  year         = {2009},
}

Chicago
Pozo Mendoza, Oscar Juan, Koen Deventer, Peter Van Eenoo, Robert Rubens, and Frans Delbeke. 2009. “Quantification of Testosterone Undecanoate in Human Hair by Liquid Chromatography-tandem Mass Spectrometry.” Biomedical Chromatography 23 (8): 873–880.
APA
Pozo Mendoza, O. J., Deventer, K., Van Eenoo, P., Rubens, R., & Delbeke, F. (2009). Quantification of testosterone undecanoate in human hair by liquid chromatography-tandem mass spectrometry. BIOMEDICAL CHROMATOGRAPHY, 23(8), 873–880.
Vancouver
1.
Pozo Mendoza OJ, Deventer K, Van Eenoo P, Rubens R, Delbeke F. Quantification of testosterone undecanoate in human hair by liquid chromatography-tandem mass spectrometry. BIOMEDICAL CHROMATOGRAPHY. 2009;23(8):873–80.
MLA
Pozo Mendoza, Oscar Juan, Koen Deventer, Peter Van Eenoo, et al. “Quantification of Testosterone Undecanoate in Human Hair by Liquid Chromatography-tandem Mass Spectrometry.” BIOMEDICAL CHROMATOGRAPHY 23.8 (2009): 873–880. Print.