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Systematic analysis of cell-cycle gene expression during Arabidopsis development

Janice de Almeida Engler (UGent) , Lieven De Veylder (UGent) , Ruth De Groodt, Stephane Rombauts (UGent) , Véronique Boudolf (UGent) , Björn De Meyer (UGent) , Adriana Hemerly (UGent) , Paulo Ferreira, Tom Beeckman (UGent) , Mansour Karimi (UGent) , et al.
(2009) Plant Journal. 59(4). p.645-660
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Abstract
The steady-state distribution of cell-cycle transcripts in Arabidopsis thaliana seedlings was studied in a broad in situ survey to provide a better understanding of the expression of cell-cycle genes during plant development. The 61 core cell-cycle genes analyzed were expressed at variable levels throughout the different plant tissues: 23 genes generally in dividing and young differentiating tissues, 34 genes mostly in both dividing and differentiated tissues and four gene transcripts primarily in differentiated tissues. Only 21 genes had a typical patchy expression pattern, indicating tight cell-cycle regulation. The increased expression of 27 cell-cycle genes in the root elongation zone hinted at their involvement in the switch from cell division to differentiation. The induction of 20 cell-cycle genes in differentiated cortical cells of etiolated hypocotyls pointed to their possible role in the process of endoreduplication. Of seven cyclin-dependent kinase inhibitor genes, five were upregulated in etiolated hypocotyls, suggesting a role in cell-cycle arrest. Nineteen genes were preferentially expressed in pericycle cells activated by auxin that give rise to lateral root primordia. Approximately 1800 images have been collected and can be queried via an online database. Our in situ analysis revealed that 70% of the cell-cycle genes, although expressed at different levels, show a large overlap in their localization. The lack of regulatory motifs in the upstream regions of the analyzed genes suggests the absence of a universal transcriptional control mechanism for all cell-cycle genes.
Keywords
cell cycle, Arabidopsis thaliana, lateral root initiation, in situ hybridization, endoreduplication

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MLA
de Almeida Engler, Janice, et al. “Systematic Analysis of Cell-Cycle Gene Expression during Arabidopsis Development.” Plant Journal, vol. 59, no. 4, 2009, pp. 645–60, doi:10.1111/j.1365-313X.2009.03893.x.
APA
de Almeida Engler, J., De Veylder, L., De Groodt, R., Rombauts, S., Boudolf, V., De Meyer, B., … Engler, G. (2009). Systematic analysis of cell-cycle gene expression during Arabidopsis development. Plant Journal, 59(4), 645–660. https://doi.org/10.1111/j.1365-313X.2009.03893.x
Chicago author-date
Almeida Engler, Janice de, Lieven De Veylder, Ruth De Groodt, Stephane Rombauts, Véronique Boudolf, Björn De Meyer, Adriana Hemerly, et al. 2009. “Systematic Analysis of Cell-Cycle Gene Expression during Arabidopsis Development.” Plant Journal 59 (4): 645–60. https://doi.org/10.1111/j.1365-313X.2009.03893.x.
Chicago author-date (all authors)
de Almeida Engler, Janice, Lieven De Veylder, Ruth De Groodt, Stephane Rombauts, Véronique Boudolf, Björn De Meyer, Adriana Hemerly, Paulo Ferreira, Tom Beeckman, Mansour Karimi, Pierre Hilson, Dirk Inzé, and Gilbert Engler. 2009. “Systematic Analysis of Cell-Cycle Gene Expression during Arabidopsis Development.” Plant Journal 59 (4): 645–660. doi:10.1111/j.1365-313X.2009.03893.x.
Vancouver
1.
de Almeida Engler J, De Veylder L, De Groodt R, Rombauts S, Boudolf V, De Meyer B, et al. Systematic analysis of cell-cycle gene expression during Arabidopsis development. Plant Journal. 2009;59(4):645–60.
IEEE
[1]
J. de Almeida Engler et al., “Systematic analysis of cell-cycle gene expression during Arabidopsis development,” Plant Journal, vol. 59, no. 4, pp. 645–660, 2009.
@article{748942,
  abstract     = {{The steady-state distribution of cell-cycle transcripts in Arabidopsis thaliana seedlings was studied in a broad in situ survey to provide a better understanding of the expression of cell-cycle genes during plant development. The 61 core cell-cycle genes analyzed were expressed at variable levels throughout the different plant tissues: 23 genes generally in dividing and young differentiating tissues, 34 genes mostly in both dividing and differentiated tissues and four gene transcripts primarily in differentiated tissues. Only 21 genes had a typical patchy expression pattern, indicating tight cell-cycle regulation. The increased expression of 27 cell-cycle genes in the root elongation zone hinted at their involvement in the switch from cell division to differentiation. The induction of 20 cell-cycle genes in differentiated cortical cells of etiolated hypocotyls pointed to their possible role in the process of endoreduplication. Of seven cyclin-dependent kinase inhibitor genes, five were upregulated in etiolated hypocotyls, suggesting a role in cell-cycle arrest. Nineteen genes were preferentially expressed in pericycle cells activated by auxin that give rise to lateral root primordia. Approximately 1800 images have been collected and can be queried via an online database. Our in situ analysis revealed that 70% of the cell-cycle genes, although expressed at different levels, show a large overlap in their localization. The lack of regulatory motifs in the upstream regions of the analyzed genes suggests the absence of a universal transcriptional control mechanism for all cell-cycle genes.}},
  author       = {{de Almeida Engler, Janice and De Veylder, Lieven and De Groodt, Ruth and Rombauts, Stephane and Boudolf, Véronique and De Meyer, Björn and Hemerly, Adriana and Ferreira, Paulo and Beeckman, Tom and Karimi, Mansour and Hilson, Pierre and Inzé, Dirk and Engler, Gilbert}},
  issn         = {{0960-7412}},
  journal      = {{Plant Journal}},
  keywords     = {{cell cycle,Arabidopsis thaliana,lateral root initiation,in situ hybridization,endoreduplication}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{645--660}},
  title        = {{Systematic analysis of cell-cycle gene expression during Arabidopsis development}},
  url          = {{http://doi.org/10.1111/j.1365-313X.2009.03893.x}},
  volume       = {{59}},
  year         = {{2009}},
}
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