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CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset

Véronique Boudolf (UGent) , Tim Lammens (UGent) , Joanna Boruc (UGent) , Jelle Van Leene (UGent) , Hilde Van Den Daele (UGent) , Sara Maes (UGent) , Gert Van Isterdael (UGent) , Eugenia Russinova (UGent) , Eva Kondorosi, Erwin Witters, et al.
(2009) Plant Physiology. 150(3). p.1482-1493
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Abstract
The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.
Keywords
CYCLIN-DEPENDENT-KINASE, ANAPHASE-PROMOTING COMPLEX, CELL-CYCLE, ARABIDOPSIS-THALIANA, TRANSCRIPTION FACTOR, S-PHASE, FISSION YEAST, PLANT DEVELOPMENT, ENDOREDUPLICATION, DESTRUCTION BOX

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MLA
Boudolf, Véronique et al. “CDKB1;1 Forms a Functional Complex with CYCA2;3 to Suppress Endocycle Onset.” Plant Physiology 150.3 (2009): 1482–1493. Print.
APA
Boudolf, V., Lammens, T., Boruc, J., Van Leene, J., Van Den Daele, H., Maes, S., Van Isterdael, G., et al. (2009). CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset. Plant Physiology, 150(3), 1482–1493.
Chicago author-date
Boudolf, Véronique, Tim Lammens, Joanna Boruc, Jelle Van Leene, Hilde Van Den Daele, Sara Maes, Gert Van Isterdael, et al. 2009. “CDKB1;1 Forms a Functional Complex with CYCA2;3 to Suppress Endocycle Onset.” Plant Physiology 150 (3): 1482–1493.
Chicago author-date (all authors)
Boudolf, Véronique, Tim Lammens, Joanna Boruc, Jelle Van Leene, Hilde Van Den Daele, Sara Maes, Gert Van Isterdael, Eugenia Russinova, Eva Kondorosi, Erwin Witters, Geert De Jaeger, Dirk Inzé, and Lieven De Veylder. 2009. “CDKB1;1 Forms a Functional Complex with CYCA2;3 to Suppress Endocycle Onset.” Plant Physiology 150 (3): 1482–1493.
Vancouver
1.
Boudolf V, Lammens T, Boruc J, Van Leene J, Van Den Daele H, Maes S, et al. CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset. Plant Physiology. 2009;150(3):1482–93.
IEEE
[1]
V. Boudolf et al., “CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset,” Plant Physiology, vol. 150, no. 3, pp. 1482–1493, 2009.
@article{748929,
  abstract     = {The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.},
  author       = {Boudolf, Véronique and Lammens, Tim and Boruc, Joanna and Van Leene, Jelle and Van Den Daele, Hilde and Maes, Sara and Van Isterdael, Gert and Russinova, Eugenia and Kondorosi, Eva and Witters, Erwin and De Jaeger, Geert and Inzé, Dirk and De Veylder, Lieven},
  issn         = {0032-0889},
  journal      = {Plant Physiology},
  keywords     = {CYCLIN-DEPENDENT-KINASE,ANAPHASE-PROMOTING COMPLEX,CELL-CYCLE,ARABIDOPSIS-THALIANA,TRANSCRIPTION FACTOR,S-PHASE,FISSION YEAST,PLANT DEVELOPMENT,ENDOREDUPLICATION,DESTRUCTION BOX},
  language     = {eng},
  number       = {3},
  pages        = {1482--1493},
  title        = {CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset},
  url          = {http://dx.doi.org/10.1104/pp.109.140269},
  volume       = {150},
  year         = {2009},
}

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