The food contaminant fumonisin B-1 reduces the maturation of porcine CD11R1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection
- Author
- Bert Devriendt (UGent) , Melanie Gallois, Frank Verdonck, Yann Wache, Diane Bimczok, Isabelle P Oswald, Bruno Goddeeris (UGent) and Eric Cox (UGent)
- Organization
- Abstract
- Consumption of food or feed contaminated with fumonisin B-1 (FB1), a mycotoxin produced by Fusarium verticillioides, can lead to disease in humans and animals. The present study was conducted to examine the effect of FB1 intake on the intestinal immune system. Piglets were used as a target and as a model species for humans since their gastro-intestinal tract is very similar. The animals were orally exposed to a low dose of FB1 (1 mg/kg body weight FB1) for 10 days which did not result in clinical signs. However, when compared to non-exposed animals, FB1-exposed animals showed a longer shedding of F4(+) enterotoxigenic Escherichia coli (ETEC) following infection and a lower induction of the antigen-specific immune response following oral immunization. Further analyses to elucidate the mechanisms behind these observations revealed a reduced intestinal expression of IL-12p40, an impaired function of intestinal antigen presenting cells (APC), with decreased upregulation of Major Histocompatibility Complex Class II molecule (MHC-II) and reduced T cell stimulatory capacity upon stimulation. Taken together, these results indicate an FB1-mediated reduction of in vivo APC maturation.
- Keywords
- DENDRITIC CELLS, mucosal immunization, ESCHERICHIA-COLI, MONOCLONAL-ANTIBODIES, ORAL IMMUNIZATION, CYTOKINE EXPRESSION, F4 FIMBRIAE, PIGS, IN-VITRO, MYCOTOXINS, EXPOSURE, F4(K88)+ enterotoxigenic Escherichia coli, fumonisin B-1, intestinal APC
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-748404
- MLA
- Devriendt, Bert, et al. “The Food Contaminant Fumonisin B-1 Reduces the Maturation of Porcine CD11R1(+) Intestinal Antigen Presenting Cells and Antigen-Specific Immune Responses, Leading to a Prolonged Intestinal ETEC Infection.” VETERINARY RESEARCH, vol. 40, no. 4, 2009, doi:10.1051/vetres/2009023.
- APA
- Devriendt, B., Gallois, M., Verdonck, F., Wache, Y., Bimczok, D., Oswald, I. P., … Cox, E. (2009). The food contaminant fumonisin B-1 reduces the maturation of porcine CD11R1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection. VETERINARY RESEARCH, 40(4). https://doi.org/10.1051/vetres/2009023
- Chicago author-date
- Devriendt, Bert, Melanie Gallois, Frank Verdonck, Yann Wache, Diane Bimczok, Isabelle P Oswald, Bruno Goddeeris, and Eric Cox. 2009. “The Food Contaminant Fumonisin B-1 Reduces the Maturation of Porcine CD11R1(+) Intestinal Antigen Presenting Cells and Antigen-Specific Immune Responses, Leading to a Prolonged Intestinal ETEC Infection.” VETERINARY RESEARCH 40 (4). https://doi.org/10.1051/vetres/2009023.
- Chicago author-date (all authors)
- Devriendt, Bert, Melanie Gallois, Frank Verdonck, Yann Wache, Diane Bimczok, Isabelle P Oswald, Bruno Goddeeris, and Eric Cox. 2009. “The Food Contaminant Fumonisin B-1 Reduces the Maturation of Porcine CD11R1(+) Intestinal Antigen Presenting Cells and Antigen-Specific Immune Responses, Leading to a Prolonged Intestinal ETEC Infection.” VETERINARY RESEARCH 40 (4). doi:10.1051/vetres/2009023.
- Vancouver
- 1.Devriendt B, Gallois M, Verdonck F, Wache Y, Bimczok D, Oswald IP, et al. The food contaminant fumonisin B-1 reduces the maturation of porcine CD11R1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection. VETERINARY RESEARCH. 2009;40(4).
- IEEE
- [1]B. Devriendt et al., “The food contaminant fumonisin B-1 reduces the maturation of porcine CD11R1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection,” VETERINARY RESEARCH, vol. 40, no. 4, 2009.
@article{748404, abstract = {{Consumption of food or feed contaminated with fumonisin B-1 (FB1), a mycotoxin produced by Fusarium verticillioides, can lead to disease in humans and animals. The present study was conducted to examine the effect of FB1 intake on the intestinal immune system. Piglets were used as a target and as a model species for humans since their gastro-intestinal tract is very similar. The animals were orally exposed to a low dose of FB1 (1 mg/kg body weight FB1) for 10 days which did not result in clinical signs. However, when compared to non-exposed animals, FB1-exposed animals showed a longer shedding of F4(+) enterotoxigenic Escherichia coli (ETEC) following infection and a lower induction of the antigen-specific immune response following oral immunization. Further analyses to elucidate the mechanisms behind these observations revealed a reduced intestinal expression of IL-12p40, an impaired function of intestinal antigen presenting cells (APC), with decreased upregulation of Major Histocompatibility Complex Class II molecule (MHC-II) and reduced T cell stimulatory capacity upon stimulation. Taken together, these results indicate an FB1-mediated reduction of in vivo APC maturation.}}, author = {{Devriendt, Bert and Gallois, Melanie and Verdonck, Frank and Wache, Yann and Bimczok, Diane and Oswald, Isabelle P and Goddeeris, Bruno and Cox, Eric}}, issn = {{0928-4249}}, journal = {{VETERINARY RESEARCH}}, keywords = {{DENDRITIC CELLS,mucosal immunization,ESCHERICHIA-COLI,MONOCLONAL-ANTIBODIES,ORAL IMMUNIZATION,CYTOKINE EXPRESSION,F4 FIMBRIAE,PIGS,IN-VITRO,MYCOTOXINS,EXPOSURE,F4(K88)+ enterotoxigenic Escherichia coli,fumonisin B-1,intestinal APC}}, language = {{eng}}, number = {{4}}, title = {{The food contaminant fumonisin B-1 reduces the maturation of porcine CD11R1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection}}, url = {{http://doi.org/10.1051/vetres/2009023}}, volume = {{40}}, year = {{2009}}, }
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