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Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows

P. Vyt, Dominiek Maes UGent, C. Quinten, Tom Rijsselaere UGent, W. Deley, M. Aarts, Aart de Kruif UGent and Ann Van Soom UGent (2008) VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT. 77(5). p.291-298
abstract
Reliable estimates of boar fertility potential from semen evaluation could be a valuable tool for boar selection. The aim of this study was to investigate the morphology and the detailed motility parameters of diluted boar semen and to relate these to their predictive value concerning conception and farrowing rate, litter size and the number of live born piglets. In addition, the optimal time for evaluation of the motility of preserved semen with respect to its predictive effect on fertility was determined. One hundred ejaculates from 38 boars were evaluated morphologically by eosin-nigrosin staining and different motility characteristics were assessed using Computer Assisted Semen Analysis (CASA). The motility was determined at 15, 45 and 120 minutes after incubation at 37 degrees C. The conception rate, farrowing rate, litter size and number of live born piglets were registered from 276 sows inseminated with these ejaculates. Different regression models were used to evaluate the predictive value of the semen characteristics on these fertility parameters, taking into account the effect of herd, parity and weaning to estrus interval. The motility characteristics of the spermatozoa varied significantly during the 15 to 120 minutes of incubation. The longer the incubation time, the more the velocity parameters along the actual cell path decreased, while the parameters of straightforward movement increased. The predictive value of individual semen parameters on conception and farrowing rate was very small. The predictive value of certain associations of different semen parameters, on the other hand, was significant. The percentage of motile spermatozoa had a significant (P<0.05) and positive effect on the total number of piglets born (litter size) and on the number of live born piglets, independent of the time of measurement (X-2 0.38-1.00 and 0.41-1.00, respectively). Accurate evaluation of the motility of a semen dose is therefore imperative for estimating its predictive value relating to fertility. In conclusion, since the time of evaluation after warming the samples significantly influences the motility parameters, CASA measurement should be done when the cells are completely acclimatized to 37 degrees C. On the basis of the available data, a 45 min incubation period appeared to be sufficient. The percentage of motile spermatozoa, as assessed by CASA on diluted semen, offers detailed predictive information regarding litter size, irrespective of the time of measurement.
Please use this url to cite or link to this publication:
author
organization
alternative title
Voorspellende waarde van spermabeweeglijkheid bij de beer voor de reproductieresultaten bij zeugen
year
type
journalArticle (original)
publication status
published
keyword
VIVO, SUBPOPULATIONS, QUALITY, PARAMETERS, SPERMATOZOA, FERTILITY, IN-VITRO, SPERM ANALYSIS
journal title
VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT
Vlaams Diergen.skund. Tijds.
volume
77
issue
5
pages
291 - 298
Web of Science type
Article
Web of Science id
000260476800002
JCR category
VETERINARY SCIENCES
JCR impact factor
0.219 (2008)
JCR rank
115/133 (2008)
JCR quartile
4 (2008)
ISSN
0303-9021
language
English
UGent publication?
yes
classification
A1
id
746864
handle
http://hdl.handle.net/1854/LU-746864
date created
2009-09-09 09:05:34
date last changed
2010-01-11 12:15:14
@article{746864,
  abstract     = {Reliable estimates of boar fertility potential from semen evaluation could be a valuable tool for boar selection. The aim of this study was to investigate the morphology and the detailed motility parameters of diluted boar semen and to relate these to their predictive value concerning conception and farrowing rate, litter size and the number of live born piglets. In addition, the optimal time for evaluation of the motility of preserved semen with respect to its predictive effect on fertility was determined. One hundred ejaculates from 38 boars were evaluated morphologically by eosin-nigrosin staining and different motility characteristics were assessed using Computer Assisted Semen Analysis (CASA). The motility was determined at 15, 45 and 120 minutes after incubation at 37 degrees C. The conception rate, farrowing rate, litter size and number of live born piglets were registered from 276 sows inseminated with these ejaculates. Different regression models were used to evaluate the predictive value of the semen characteristics on these fertility parameters, taking into account the effect of herd, parity and weaning to estrus interval.

The motility characteristics of the spermatozoa varied significantly during the 15 to 120 minutes of incubation. The longer the incubation time, the more the velocity parameters along the actual cell path decreased, while the parameters of straightforward movement increased. The predictive value of individual semen parameters on conception and farrowing rate was very small. The predictive value of certain associations of different semen parameters, on the other hand, was significant.

The percentage of motile spermatozoa had a significant (P{\textlangle}0.05) and positive effect on the total number of piglets born (litter size) and on the number of live born piglets, independent of the time of measurement (X-2 0.38-1.00 and 0.41-1.00, respectively). Accurate evaluation of the motility of a semen dose is therefore imperative for estimating its predictive value relating to fertility.

In conclusion, since the time of evaluation after warming the samples significantly influences the motility parameters, CASA measurement should be done when the cells are completely acclimatized to 37 degrees C. On the basis of the available data, a 45 min incubation period appeared to be sufficient. The percentage of motile spermatozoa, as assessed by CASA on diluted semen, offers detailed predictive information regarding litter size, irrespective of the time of measurement.},
  author       = {Vyt, P. and Maes, Dominiek and Quinten, C. and Rijsselaere, Tom and Deley, W. and Aarts, M. and de Kruif, Aart and Van Soom, Ann},
  issn         = {0303-9021},
  journal      = {VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT},
  keyword      = {VIVO,SUBPOPULATIONS,QUALITY,PARAMETERS,SPERMATOZOA,FERTILITY,IN-VITRO,SPERM ANALYSIS},
  language     = {eng},
  number       = {5},
  pages        = {291--298},
  title        = {Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows},
  volume       = {77},
  year         = {2008},
}

Chicago
Vyt, P., Dominiek Maes, C. Quinten, Tom Rijsselaere, W. Deley, M. Aarts, Aart de Kruif, and Ann Van Soom. 2008. “Detailed Motility Evaluation of Boar Semen and Its Predictive Value for Reproductive Performance in Sows.” Vlaams Diergeneeskundig Tijdschrift 77 (5): 291–298.
APA
Vyt, P., Maes, D., Quinten, C., Rijsselaere, T., Deley, W., Aarts, M., de Kruif, A., et al. (2008). Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows. VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT, 77(5), 291–298.
Vancouver
1.
Vyt P, Maes D, Quinten C, Rijsselaere T, Deley W, Aarts M, et al. Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows. VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT. 2008;77(5):291–8.
MLA
Vyt, P., Dominiek Maes, C. Quinten, et al. “Detailed Motility Evaluation of Boar Semen and Its Predictive Value for Reproductive Performance in Sows.” VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT 77.5 (2008): 291–298. Print.