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Conservation of the chitin utilization pathway in the Vibrionaceae

Dana E. Hunt, Dirk Gevers UGent, Nisha M. Vahora and Martin F. Polz (2008) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 74(1). p.44-51
abstract
Vibrionaceae are regarded as important marine chitin degraders, and attachment to chitin regulates important biological functions; yet, the degree of chitin pathway conservation in Vibrionaceae is unknown. Here, a core chitin degradation pathway is proposed based on comparison of 19 Vibrio and Photobacterium genomes with a detailed metabolic map assembled for V. cholerae from published biochemical, genomic, and transcriptomic results. Further, to assess whether chitin degradation is a conserved property of Vibrionaceae, a set of 54 strains from 32 taxa were tested for the ability to grow on various forms of chitin. All strains grew on N-acetylglucosamine (GlcNAc), the monomer of chitin. The majority of isolates grew on et (crab shell) and 0 (squid pen) chitin and contained chitinase A (chiA) genes. chiA sequencing and phylogenetic analysis suggest that this gene is a good indicator of chitin metabolism but appears subject to horizontal gene transfer and duplication. Overall, chitin metabolism appears to be a core function of Vibrionaceae, but individual pathway components exhibit dynamic evolutionary histories.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
keyword
CATABOLIC CASCADE, ENVIRONMENT, ORGANIC NITROGEN, CHESAPEAKE BAY, CHOLERAE, FURNISSII, IDENTIFICATION, DEGRADATION, MARINE-BACTERIA, MOLECULAR-CLONING
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
74
issue
1
pages
44 - 51
Web of Science type
Article
Web of Science id
000252195200004
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.801 (2008)
JCR rank
27/144 (2008)
JCR quartile
1 (2008)
ISSN
0099-2240
DOI
10.1128/AEM.01412-07
language
English
UGent publication?
yes
classification
A1
id
744581
handle
http://hdl.handle.net/1854/LU-744581
date created
2009-09-09 09:01:55
date last changed
2009-10-27 09:09:20
@article{744581,
  abstract     = {Vibrionaceae are regarded as important marine chitin degraders, and attachment to chitin regulates important biological functions; yet, the degree of chitin pathway conservation in Vibrionaceae is unknown. Here, a core chitin degradation pathway is proposed based on comparison of 19 Vibrio and Photobacterium genomes with a detailed metabolic map assembled for V. cholerae from published biochemical, genomic, and transcriptomic results. Further, to assess whether chitin degradation is a conserved property of Vibrionaceae, a set of 54 strains from 32 taxa were tested for the ability to grow on various forms of chitin. All strains grew on N-acetylglucosamine (GlcNAc), the monomer of chitin. The majority of isolates grew on et (crab shell) and 0 (squid pen) chitin and contained chitinase A (chiA) genes. chiA sequencing and phylogenetic analysis suggest that this gene is a good indicator of chitin metabolism but appears subject to horizontal gene transfer and duplication. Overall, chitin metabolism appears to be a core function of Vibrionaceae, but individual pathway components exhibit dynamic evolutionary histories.},
  author       = {Hunt, Dana E. and Gevers, Dirk and Vahora, Nisha M. and Polz, Martin F.},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {CATABOLIC CASCADE,ENVIRONMENT,ORGANIC NITROGEN,CHESAPEAKE BAY,CHOLERAE,FURNISSII,IDENTIFICATION,DEGRADATION,MARINE-BACTERIA,MOLECULAR-CLONING},
  language     = {eng},
  number       = {1},
  pages        = {44--51},
  title        = {Conservation of the chitin utilization pathway in the Vibrionaceae},
  url          = {http://dx.doi.org/10.1128/AEM.01412-07},
  volume       = {74},
  year         = {2008},
}

Chicago
Hunt, Dana E., Dirk Gevers, Nisha M. Vahora, and Martin F. Polz. 2008. “Conservation of the Chitin Utilization Pathway in the Vibrionaceae.” Applied and Environmental Microbiology 74 (1): 44–51.
APA
Hunt, D. E., Gevers, D., Vahora, N. M., & Polz, M. F. (2008). Conservation of the chitin utilization pathway in the Vibrionaceae. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(1), 44–51.
Vancouver
1.
Hunt DE, Gevers D, Vahora NM, Polz MF. Conservation of the chitin utilization pathway in the Vibrionaceae. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2008;74(1):44–51.
MLA
Hunt, Dana E., Dirk Gevers, Nisha M. Vahora, et al. “Conservation of the Chitin Utilization Pathway in the Vibrionaceae.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 74.1 (2008): 44–51. Print.