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Phylogenetic analysis of vibrios and related species by means of atpA gene sequences

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Abstract
We investigated the use of atpA gene sequences as alternative phylogenetic and identification markers for vibrios. A fragment of 1322 bp (corresponding to approximately 88% of the coding region) was analysed in 151 strains of vibrios. The relationships observed were in agreement with the phylogeny inferred from 16S rRNA gene sequence analysis. For instance, the Vibrio cholerae, Vibrio halioticoli, Vibrio harveyi and Vibrio splendidus species groups appeared in the atpA gene phylogenetic analyses, suggesting that these groups may be considered as separate genera within the current Vibrio genus. Overall, atpA gene sequences appeared to be more discriminatory for species differentiation than 16S rRNA gene sequences. 16S rRNA gene sequence similarities above 97% corresponded to atpA gene sequences similarities above 80%. The intraspecies variation in the atpA gene sequence was about 99% sequence similarity. The results showed clearly that atpA gene sequences are a suitable alternative for the identification and phylogenetic study of vibrios.
Keywords
IDENTIFICATION, SP-NOV., DIVERSITY, ALIGNMENT, HORIZONTAL TRANSFER, FRAGMENT

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MLA
Thompson, Cristiane C. et al. “Phylogenetic Analysis of Vibrios and Related Species by Means of atpA Gene Sequences.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 57.11 (2007): 2480–2484. Print.
APA
Thompson, C. C., Thompson, F. L., Vicente, A. C. P., & Swings, J. (2007). Phylogenetic analysis of vibrios and related species by means of atpA gene sequences. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 57(11), 2480–2484.
Chicago author-date
Thompson, Cristiane C., Fabiano L. Thompson, Ana Carolina P. Vicente, and Jean Swings. 2007. “Phylogenetic Analysis of Vibrios and Related Species by Means of atpA Gene Sequences.” International Journal of Systematic and Evolutionary Microbiology 57 (11): 2480–2484.
Chicago author-date (all authors)
Thompson, Cristiane C., Fabiano L. Thompson, Ana Carolina P. Vicente, and Jean Swings. 2007. “Phylogenetic Analysis of Vibrios and Related Species by Means of atpA Gene Sequences.” International Journal of Systematic and Evolutionary Microbiology 57 (11): 2480–2484.
Vancouver
1.
Thompson CC, Thompson FL, Vicente ACP, Swings J. Phylogenetic analysis of vibrios and related species by means of atpA gene sequences. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2007;57(11):2480–4.
IEEE
[1]
C. C. Thompson, F. L. Thompson, A. C. P. Vicente, and J. Swings, “Phylogenetic analysis of vibrios and related species by means of atpA gene sequences,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 57, no. 11, pp. 2480–2484, 2007.
@article{744372,
  abstract     = {{We investigated the use of atpA gene sequences as alternative phylogenetic and identification markers for vibrios. A fragment of 1322 bp (corresponding to approximately 88% of the coding region) was analysed in 151 strains of vibrios. The relationships observed were in agreement with the phylogeny inferred from 16S rRNA gene sequence analysis. For instance, the Vibrio cholerae, Vibrio halioticoli, Vibrio harveyi and Vibrio splendidus species groups appeared in the atpA gene phylogenetic analyses, suggesting that these groups may be considered as separate genera within the current Vibrio genus. Overall, atpA gene sequences appeared to be more discriminatory for species differentiation than 16S rRNA gene sequences. 16S rRNA gene sequence similarities above 97% corresponded to atpA gene sequences similarities above 80%. The intraspecies variation in the atpA gene sequence was about 99% sequence similarity. The results showed clearly that atpA gene sequences are a suitable alternative for the identification and phylogenetic study of vibrios.}},
  author       = {{Thompson, Cristiane C. and Thompson, Fabiano L. and Vicente, Ana Carolina P. and Swings, Jean}},
  issn         = {{1466-5026}},
  journal      = {{INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}},
  keywords     = {{IDENTIFICATION,SP-NOV.,DIVERSITY,ALIGNMENT,HORIZONTAL TRANSFER,FRAGMENT}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{2480--2484}},
  title        = {{Phylogenetic analysis of vibrios and related species by means of atpA gene sequences}},
  url          = {{http://dx.doi.org/10.1099/ijs.0.65223-0}},
  volume       = {{57}},
  year         = {{2007}},
}

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