Advanced search
1 file | 1.38 MB Add to list

Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration

Thomas Hubert (UGent) , Joël Vandekerckhove (UGent) and Jan Gettemans (UGent)
(2009) TRAFFIC. 10(9). p.1257-1271
Author
Organization
Abstract
Nucleoporin Nup62 localizes at the central channel of the nuclear pore complex and is essential for nucleocytoplasmic transport. Through its FG-repeat domain, Nup62 regulates nuclear pore permeability and binds nuclear transport receptors. Here, we report that Nup62 interacts directly with Exo70 and colocalizes with Exo70 at the leading edge of migrating cells. Nup62 binds the N-terminal domain of Exo70 through its coiled-coil domain but not through its FG-repeat domain. Selective inhibition of leading edge Nup62 using RNA interference significantly reduces cell migration. Furthermore, Exo70 recruits Nup62 at the plasma membrane and at filopodia. Removal of the Exo70-binding domain of Nup62 prevents leading edge localization of Nup62. Analogous to Exo70, Nup62 cycles between the plasma membrane and the perinuclear recycling compartment. Altogether, we propose that Nup62 not solely regulates access to the cell nucleus, but additionally functions in conjunction with Exo70, a key regulator of exocytosis and actin dynamics, at the leading edge of migrating cells.
Keywords
STRUCTURAL BASIS, TRANSPORT FACTOR-2, 5-BISPHOSPHATE, PHOSPHATIDYLINOSITOL 4, EXOCYST COMPLEX, PLASMA-MEMBRANE, Nup62, KeyWords Plus: NUCLEAR-PORE COMPLEX, nucleoporin, leading edge, cell migration, Exo70, MAMMALIAN-CELLS, EXO70 INTERACTS, LIVING CELLS, DNA-DAMAGE

Downloads

  • 523-2009 Hubert T. exo70-mediated... Traffic.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.38 MB

Citation

Please use this url to cite or link to this publication:

MLA
Hubert, Thomas, Joël Vandekerckhove, and Jan Gettemans. “Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells Is Required for Cell Migration.” TRAFFIC 10.9 (2009): 1257–1271. Print.
APA
Hubert, T., Vandekerckhove, J., & Gettemans, J. (2009). Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration. TRAFFIC, 10(9), 1257–1271.
Chicago author-date
Hubert, Thomas, Joël Vandekerckhove, and Jan Gettemans. 2009. “Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells Is Required for Cell Migration.” Traffic 10 (9): 1257–1271.
Chicago author-date (all authors)
Hubert, Thomas, Joël Vandekerckhove, and Jan Gettemans. 2009. “Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells Is Required for Cell Migration.” Traffic 10 (9): 1257–1271.
Vancouver
1.
Hubert T, Vandekerckhove J, Gettemans J. Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration. TRAFFIC. MALDEN 02148, MA USA: WILEY-BLACKWELL PUBLISHING, INC; 2009;10(9):1257–71.
IEEE
[1]
T. Hubert, J. Vandekerckhove, and J. Gettemans, “Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration,” TRAFFIC, vol. 10, no. 9, pp. 1257–1271, 2009.
@article{734118,
  abstract     = {Nucleoporin Nup62 localizes at the central channel of the nuclear pore complex and is essential for nucleocytoplasmic transport. Through its FG-repeat domain, Nup62 regulates nuclear pore permeability and binds nuclear transport receptors. Here, we report that Nup62 interacts directly with Exo70 and colocalizes with Exo70 at the leading edge of migrating cells. Nup62 binds the N-terminal domain of Exo70 through its coiled-coil domain but not through its FG-repeat domain. Selective inhibition of leading edge Nup62 using RNA interference significantly reduces cell migration. Furthermore, Exo70 recruits Nup62 at the plasma membrane and at filopodia. Removal of the Exo70-binding domain of Nup62 prevents leading edge localization of Nup62. Analogous to Exo70, Nup62 cycles between the plasma membrane and the perinuclear recycling compartment. Altogether, we propose that Nup62 not solely regulates access to the cell nucleus, but additionally functions in conjunction with Exo70, a key regulator of exocytosis and actin dynamics, at the leading edge of migrating cells.},
  author       = {Hubert, Thomas and Vandekerckhove, Joël and Gettemans, Jan},
  issn         = {1398-9219},
  journal      = {TRAFFIC},
  keywords     = {STRUCTURAL BASIS,TRANSPORT FACTOR-2,5-BISPHOSPHATE,PHOSPHATIDYLINOSITOL 4,EXOCYST COMPLEX,PLASMA-MEMBRANE,Nup62,KeyWords Plus: NUCLEAR-PORE COMPLEX,nucleoporin,leading edge,cell migration,Exo70,MAMMALIAN-CELLS,EXO70 INTERACTS,LIVING CELLS,DNA-DAMAGE},
  language     = {eng},
  number       = {9},
  pages        = {1257--1271},
  publisher    = {WILEY-BLACKWELL PUBLISHING, INC},
  title        = {Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration},
  url          = {http://dx.doi.org/10.1111/j.1600-0854.2009.00940.x},
  volume       = {10},
  year         = {2009},
}

Altmetric
View in Altmetric
Web of Science
Times cited: