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In vitro study of red clover polyphenol oxidase activity, activation, and effect on measured lipase activity and lipolysis

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Abstract
The goal of this paper was, first, to study the effect of red clover polyphenol oxidase (PPO) activity on protein-bound phenols and measured lipase activity in vitro and, second, to study the effect of PPO activation, measured as an increase in protein-bound phenols, as a result of degrees of damaging (not damaged, crushed, and freeze/thawed) of red clover before wilting on measured enzyme activity and in vitro lipid metabolism when incubated in a phosphate buffer. There was a positive relation between PPO activity and the occurrence of protein-bound phenols with a concomitant decrease in measured lipase activity, indicating a possibility to a direct inhibition of enzymes as a result of protein-bound phenols. Furthermore, damaging can activate PPO in red clover, measured as an increase in protein-bound phenols during wilting [0.7-20.6 nmol of tyrosine equiv (mg of protein)(-1)], again with a concomitant decrease in measured lipase activity [41.3-20.3 mu mol of p-nitrophenyl butyrate (PNPB) min(-1) (mg of protein)(-1)]. Lipid metabolism during incubation of these forages in a phosphate buffer with ascorbic acid was only influenced by damaging when wilted for 24 h, with a lower lipolysis in crushed and freeze/thawed (52.9 and 32.6%, respectively, after 8 h of incubation) material compared to all other treatments (on average 60.4% after 8 h of incubation).
Keywords
red clover, polyphenol oxidase, lipid metabolism, Silage, fatty acids, FATTY-ACID-COMPOSITION, LIPID-METABOLISM, ENZYME-ACTIVITY, PROTEOLYSIS, L., PURIFICATION, ADDITIVES, PROTEINS, PHENOLS, FORAGES

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Chicago
Van Ranst, Gijs, Veerle Fievez, Murielle Vandewalle, Jan De Riek, and Erik Van Bockstaele. 2009. “In Vitro Study of Red Clover Polyphenol Oxidase Activity, Activation, and Effect on Measured Lipase Activity and Lipolysis.” Journal of Agricultural and Food Chemistry 57 (15): 6611–6617.
APA
Van Ranst, G., Fievez, V., Vandewalle, M., De Riek, J., & Van Bockstaele, E. (2009). In vitro study of red clover polyphenol oxidase activity, activation, and effect on measured lipase activity and lipolysis. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 57(15), 6611–6617.
Vancouver
1.
Van Ranst G, Fievez V, Vandewalle M, De Riek J, Van Bockstaele E. In vitro study of red clover polyphenol oxidase activity, activation, and effect on measured lipase activity and lipolysis. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2009;57(15):6611–7.
MLA
Van Ranst, Gijs, Veerle Fievez, Murielle Vandewalle, et al. “In Vitro Study of Red Clover Polyphenol Oxidase Activity, Activation, and Effect on Measured Lipase Activity and Lipolysis.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 57.15 (2009): 6611–6617. Print.
@article{733515,
  abstract     = {The goal of this paper was, first, to study the effect of red clover polyphenol oxidase (PPO) activity on protein-bound phenols and measured lipase activity in vitro and, second, to study the effect of PPO activation, measured as an increase in protein-bound phenols, as a result of degrees of damaging (not damaged, crushed, and freeze/thawed) of red clover before wilting on measured enzyme activity and in vitro lipid metabolism when incubated in a phosphate buffer. There was a positive relation between PPO activity and the occurrence of protein-bound phenols with a concomitant decrease in measured lipase activity, indicating a possibility to a direct inhibition of enzymes as a result of protein-bound phenols. Furthermore, damaging can activate PPO in red clover, measured as an increase in protein-bound phenols during wilting [0.7-20.6 nmol of tyrosine equiv (mg of protein)(-1)], again with a concomitant decrease in measured lipase activity [41.3-20.3 mu mol of p-nitrophenyl butyrate (PNPB) min(-1) (mg of protein)(-1)]. Lipid metabolism during incubation of these forages in a phosphate buffer with ascorbic acid was only influenced by damaging when wilted for 24 h, with a lower lipolysis in crushed and freeze/thawed (52.9 and 32.6\%, respectively, after 8 h of incubation) material compared to all other treatments (on average 60.4\% after 8 h of incubation).},
  author       = {Van Ranst, Gijs and Fievez, Veerle and Vandewalle, Murielle and De Riek, Jan and Van Bockstaele, Erik},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {red clover,polyphenol oxidase,lipid metabolism,Silage,fatty acids,FATTY-ACID-COMPOSITION,LIPID-METABOLISM,ENZYME-ACTIVITY,PROTEOLYSIS,L.,PURIFICATION,ADDITIVES,PROTEINS,PHENOLS,FORAGES},
  language     = {eng},
  number       = {15},
  pages        = {6611--6617},
  title        = {In vitro study of red clover polyphenol oxidase activity, activation, and effect on measured lipase activity and lipolysis},
  url          = {http://dx.doi.org/10.1021/jf900808p},
  volume       = {57},
  year         = {2009},
}

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