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Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes

(2009) PARASITOLOGY RESEARCH. 105(3). p.825-834
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Abstract
The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described.
Keywords
ANTHELMINTIC RESISTANCE, IN-VITRO METHODS, VETERINARY IMPORTANCE, HAEMONCHUS-CONTORTUS, LEVAMISOLE RESISTANCE, SMALL STRONGYLES, COUNT REDUCTION TEST, NORTH-ISLAND, NEW-ZEALAND, SHEEP

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Citation

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Chicago
von Samson-Himmelstjerna, Georg, Gerald C Coles, Frank Jackson, Christian Bauer, Fred Borgsteede, Veli Y Cirak, Janina Demeler, et al. 2009. “Standardization of the Egg Hatch Test for the Detection of Benzimidazole Resistance in Parasitic Nematodes.” Parasitology Research 105 (3): 825–834.
APA
von Samson-Himmelstjerna, G., Coles, G. C., Jackson, F., Bauer, C., Borgsteede, F., Cirak, V. Y., Demeler, J., et al. (2009). Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes. PARASITOLOGY RESEARCH, 105(3), 825–834.
Vancouver
1.
von Samson-Himmelstjerna G, Coles GC, Jackson F, Bauer C, Borgsteede F, Cirak VY, et al. Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes. PARASITOLOGY RESEARCH. 2009;105(3):825–34.
MLA
von Samson-Himmelstjerna, Georg, Gerald C Coles, Frank Jackson, et al. “Standardization of the Egg Hatch Test for the Detection of Benzimidazole Resistance in Parasitic Nematodes.” PARASITOLOGY RESEARCH 105.3 (2009): 825–834. Print.
@article{731219,
  abstract     = {The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described.},
  author       = {von Samson-Himmelstjerna, Georg and Coles, Gerald C and Jackson, Frank and Bauer, Christian and Borgsteede, Fred and Cirak, Veli Y and Demeler, Janina and Donnan, Alison and Dorny, Pierre and Epe, Christian and Harder, Achim and Hoglund, Johan and Kaminsky, Ronald and Kerboeuf, Dominique and Kuttler, Ulla and Papadopoulos, Elias and Posedi, Janez and Small, John and Varady, Marian and Vercruysse, Jozef and Wirtherle, Nicole},
  issn         = {0932-0113},
  journal      = {PARASITOLOGY RESEARCH},
  keyword      = {ANTHELMINTIC RESISTANCE,IN-VITRO METHODS,VETERINARY IMPORTANCE,HAEMONCHUS-CONTORTUS,LEVAMISOLE RESISTANCE,SMALL STRONGYLES,COUNT REDUCTION TEST,NORTH-ISLAND,NEW-ZEALAND,SHEEP},
  language     = {eng},
  number       = {3},
  pages        = {825--834},
  title        = {Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes},
  url          = {http://dx.doi.org/10.1007/s00436-009-1466-1},
  volume       = {105},
  year         = {2009},
}

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