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Role of histidyl dipeptides in contractile function of fast and slow motor units in rat skeletal muscle

Dominik Kaczmarek, Dawid Łochyński, Inge Everaert UGent, Maciej Pawlak, Wim Derave UGent and Jan Celichowski (2016) JOURNAL OF APPLIED PHYSIOLOGY. 121(1). p.164-172
abstract
The physiological role of the muscle histidyl dipeptides carnosine and anserine in contractile function of various types of muscle fibres in vivo is poorly understood. Ten adult male Wistar rats were randomly assigned to two groups: control and supplemented for 10 weeks with beta-alanine, the precursor of carnosine (~640 mg/kg BW/day). Thereafter, contractile properties and fatigability of isolated fast fatigable (FF), fast resistant to fatigue (FR), and slow motor units (MUs) from the medial gastrocnemius were determined in deeply anaesthetized animals. The fatigue resistance was tested with a 40 Hz fatigue protocol followed by a second protocol at 40 Hz in fast and 20 Hz in slow units. In the supplemented rats, histidyl dipeptide concentrations significantly increased (P < 0.05) by 25% in the red portion of the gastrocnemius and carnosine increased by 94% in the white portion. The twitch force of FF units and maximum tetanic force of FR units were significantly increased (P < 0.05) and the half-relaxation time was prolonged in slow units (P < 0.05). FF units showed less fatigue during the first 10 s and FR units between 10 and 60 s during the 40 Hz fatigue test. In slow units, forces declined less during the first 60 s of the 20 Hz test. In conclusion, this in vivo experiment demonstrates that an elevation in muscle histidyl dipeptide content elicits beneficial changes in MU contractile characteristics and fatigue resistance. Carnosine and anserine seem to play an important yet divergent role in various MUs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
fatigue, rat, motor units, carnosine, beta-alanine, BETA-ALANINE SUPPLEMENTATION, MEDIAL GASTROCNEMIUS-MUSCLE, HUMAN VASTUS LATERALIS, WHOLE-BODY VIBRATION, EXERCISE PERFORMANCE, FORCE PRODUCTION, STRIATED-MUSCLE, SOLEUS MUSCLE, CARNOSINE, FATIGUE
journal title
JOURNAL OF APPLIED PHYSIOLOGY
J. Appl. Physiol.
volume
121
issue
1
pages
164 - 172
Web of Science type
Article
Web of Science id
000380750700019
JCR category
SPORT SCIENCES
JCR impact factor
3.351 (2016)
JCR rank
8/81 (2016)
JCR quartile
1 (2016)
ISSN
8750-7587
DOI
10.1152/japplphysiol.00848.2015
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7276209
handle
http://hdl.handle.net/1854/LU-7276209
date created
2016-06-21 10:20:01
date last changed
2017-07-25 11:26:56
@article{7276209,
  abstract     = {The physiological role of the muscle histidyl dipeptides carnosine and anserine in contractile function of various types of muscle fibres in vivo is poorly understood. Ten adult male Wistar rats were randomly assigned to two groups: control and supplemented for 10 weeks with beta-alanine, the precursor of carnosine ({\texttildelow}640 mg/kg BW/day). Thereafter, contractile properties and fatigability of isolated fast fatigable (FF), fast resistant to fatigue (FR), and slow motor units (MUs) from the medial gastrocnemius were determined in deeply anaesthetized animals. The fatigue resistance was tested with a 40 Hz fatigue protocol followed by a second protocol at 40 Hz in fast and 20 Hz in slow units. In the supplemented rats, histidyl dipeptide concentrations significantly increased (P {\textlangle} 0.05) by 25\% in the red portion of the gastrocnemius and carnosine increased by 94\% in the white portion. The twitch force of FF units and maximum tetanic force of FR units were significantly increased (P {\textlangle} 0.05) and the half-relaxation time was prolonged in slow units (P {\textlangle} 0.05). FF units showed less fatigue during the first 10 s and FR units between 10 and 60 s during the 40 Hz fatigue test. In slow units, forces declined less during the first 60 s of the 20 Hz test. In conclusion, this in vivo experiment demonstrates that an elevation in muscle histidyl dipeptide content elicits beneficial changes in MU contractile characteristics and fatigue resistance. Carnosine and anserine seem to play an important yet divergent role in various MUs.},
  author       = {Kaczmarek, Dominik and \unmatched{0141}ochy\'{n}ski, Dawid and Everaert, Inge and Pawlak, Maciej and Derave, Wim and Celichowski, Jan},
  issn         = {8750-7587},
  journal      = {JOURNAL OF APPLIED PHYSIOLOGY},
  keyword      = {fatigue,rat,motor units,carnosine,beta-alanine,BETA-ALANINE SUPPLEMENTATION,MEDIAL GASTROCNEMIUS-MUSCLE,HUMAN VASTUS LATERALIS,WHOLE-BODY VIBRATION,EXERCISE PERFORMANCE,FORCE PRODUCTION,STRIATED-MUSCLE,SOLEUS MUSCLE,CARNOSINE,FATIGUE},
  language     = {eng},
  number       = {1},
  pages        = {164--172},
  title        = {Role of histidyl dipeptides in contractile function of fast and slow motor units in rat skeletal muscle},
  url          = {http://dx.doi.org/10.1152/japplphysiol.00848.2015},
  volume       = {121},
  year         = {2016},
}

Chicago
Kaczmarek, Dominik, Dawid Łochyński, Inge Everaert, Maciej Pawlak, Wim Derave, and Jan Celichowski. 2016. “Role of Histidyl Dipeptides in Contractile Function of Fast and Slow Motor Units in Rat Skeletal Muscle.” Journal of Applied Physiology 121 (1): 164–172.
APA
Kaczmarek, D., Łochyński, D., Everaert, I., Pawlak, M., Derave, W., & Celichowski, J. (2016). Role of histidyl dipeptides in contractile function of fast and slow motor units in rat skeletal muscle. JOURNAL OF APPLIED PHYSIOLOGY, 121(1), 164–172.
Vancouver
1.
Kaczmarek D, Łochyński D, Everaert I, Pawlak M, Derave W, Celichowski J. Role of histidyl dipeptides in contractile function of fast and slow motor units in rat skeletal muscle. JOURNAL OF APPLIED PHYSIOLOGY. 2016;121(1):164–72.
MLA
Kaczmarek, Dominik, Dawid Łochyński, Inge Everaert, et al. “Role of Histidyl Dipeptides in Contractile Function of Fast and Slow Motor Units in Rat Skeletal Muscle.” JOURNAL OF APPLIED PHYSIOLOGY 121.1 (2016): 164–172. Print.