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The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK1/2 induced cytoskeleton reorganization

Virginie Vandenbroucke UGent, Siska Croubels UGent, Elin Verbrugghe UGent, Filip Boyen UGent, Patrick De Backer UGent, Richard Ducatelle UGent, Yvan Rychlik, Freddy Haesebrouck UGent and Frank Pasmans UGent (2009) VETERINARY RESEARCH. 40(6).
abstract
Both the mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium are major issues in swine production. This study aimed at examining the interaction between DON and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 mu g/mL of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of Salmonella Typhimurium was determined. At low non-cytotoxic concentrations, as can be found in the serum of pigs, DON did not have any effect on either growth or virulence gene expression of Salmonella Typhimurium. However, when the invasion and intracellular survival of Salmonella Typhimurium in macrophages preexposed to 0.025 mu g/mL of DON was examined, DON significantly promoted the uptake of Salmonella Typhimurium into macrophages. The enhanced uptake coincided with marked F-actin reorganization of the cells, which was due to the activation of extracellular signal-regulated protein kinase 1/2 (ERK1/2). These results suggest that low but relevant concentrations of DON modulate the innate immune system and could thus increase the susceptibility of pigs to infections with Salmonella Typhimurium.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SUPPRESSION, INFECTIONS, PIGS, KINASE, IN-VIVO, EPITHELIAL-CELLS, RIBOTOXIC STRESS-RESPONSE, FUSARIUM-TOXIN DEOXYNIVALENOL, cytoskeleton, pig, macrophage, INDUCTION, EXPRESSION, deoxynivalenol, Salmonella Typhimurium
journal title
VETERINARY RESEARCH
Vet. Res.
volume
40
issue
6
article_number
64
pages
12 pages
Web of Science type
Article
Web of Science id
000272039500013
JCR category
VETERINARY SCIENCES
JCR impact factor
3.579 (2009)
JCR rank
1/141 (2009)
JCR quartile
1 (2009)
ISSN
0928-4249
DOI
10.1051/vetres/2009045
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
727359
handle
http://hdl.handle.net/1854/LU-727359
date created
2009-08-18 15:20:14
date last changed
2012-10-31 14:12:49
@article{727359,
  abstract     = {Both the mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium are major issues in swine production. This study aimed at examining the interaction between DON and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 mu g/mL of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of Salmonella Typhimurium was determined. At low non-cytotoxic concentrations, as can be found in the serum of pigs, DON did not have any effect on either growth or virulence gene expression of Salmonella Typhimurium. However, when the invasion and intracellular survival of Salmonella Typhimurium in macrophages preexposed to 0.025 mu g/mL of DON was examined, DON significantly promoted the uptake of Salmonella Typhimurium into macrophages. The enhanced uptake coincided with marked F-actin reorganization of the cells, which was due to the activation of extracellular signal-regulated protein kinase 1/2 (ERK1/2). These results suggest that low but relevant concentrations of DON modulate the innate immune system and could thus increase the susceptibility of pigs to infections with Salmonella Typhimurium.},
  articleno    = {64},
  author       = {Vandenbroucke, Virginie and Croubels, Siska and Verbrugghe, Elin and Boyen, Filip and De Backer, Patrick and Ducatelle, Richard and Rychlik, Yvan and Haesebrouck, Freddy and Pasmans, Frank},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {SUPPRESSION,INFECTIONS,PIGS,KINASE,IN-VIVO,EPITHELIAL-CELLS,RIBOTOXIC STRESS-RESPONSE,FUSARIUM-TOXIN DEOXYNIVALENOL,cytoskeleton,pig,macrophage,INDUCTION,EXPRESSION,deoxynivalenol,Salmonella Typhimurium},
  language     = {eng},
  number       = {6},
  pages        = {12},
  title        = {The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK1/2 induced cytoskeleton reorganization},
  url          = {http://dx.doi.org/10.1051/vetres/2009045},
  volume       = {40},
  year         = {2009},
}

Chicago
Vandenbroucke, Virginie, Siska Croubels, Elin Verbrugghe, Filip Boyen, Patrick De Backer, Richard Ducatelle, Yvan Rychlik, Freddy Haesebrouck, and Frank Pasmans. 2009. “The Mycotoxin Deoxynivalenol Promotes Uptake of Salmonella Typhimurium in Porcine Macrophages, Associated with ERK1/2 Induced Cytoskeleton Reorganization.” Veterinary Research 40 (6).
APA
Vandenbroucke, V., Croubels, S., Verbrugghe, E., Boyen, F., De Backer, P., Ducatelle, R., Rychlik, Y., et al. (2009). The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK1/2 induced cytoskeleton reorganization. VETERINARY RESEARCH, 40(6).
Vancouver
1.
Vandenbroucke V, Croubels S, Verbrugghe E, Boyen F, De Backer P, Ducatelle R, et al. The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK1/2 induced cytoskeleton reorganization. VETERINARY RESEARCH. 2009;40(6).
MLA
Vandenbroucke, Virginie, Siska Croubels, Elin Verbrugghe, et al. “The Mycotoxin Deoxynivalenol Promotes Uptake of Salmonella Typhimurium in Porcine Macrophages, Associated with ERK1/2 Induced Cytoskeleton Reorganization.” VETERINARY RESEARCH 40.6 (2009): n. pag. Print.