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Quantitative determination and subcellular imaging of Cu in single cells via laser ablation-ICP-mass spectrometry using high-density microarray gelatin standards

Stijn Van Malderen UGent, Eva Vergucht, Maarten De Rijcke, Colin Janssen UGent, Laszlo Vincze UGent and Frank Vanhaecke UGent (2016) ANALYTICAL CHEMISTRY. 88(11). p.5783-5789
abstract
This manuscript describes the development and characterization of a high-density microarray calibration standard, manufactured in-house and designed to overcome the limitations in precision, accuracy, and throughput of current calibration approaches for the quantification of elemental concentrations on the cellular level using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICPMS). As a case study, the accumulation of Cu in the model organism Scrippsiella trochoidea resulting from transition metal exposure (ranging from 0.5 to 100 μg/L) was evaluated. After the Cu exposure, cells of this photosynthetic dinoflagellate were treated with a critical point drying protocol, transferred to a carbon stub, and sputter-coated with a Au layer for scanning electron microscopy (SEM) analysis. In subsequent LA-ICPMS analysis, approximately 100 cells of each population were individually ablated. This approach permitted the evaluation of the mean concentration of Cu in the cell population across different exposure levels and also allowed the examination of the cellular distribution of Cu within the populations. In a cross-validation exercise, subcellular LA-ICPMS imaging was demonstrated to corroborate synchrotron radiation confocal X-ray fluorescence (SR-XRF) microimaging of single cells investigated under in vivo conditions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
OPTICAL TWEEZERS, LIQUID SAMPLES, MODEL ORGANISMS, TISSUE-SECTIONS, CYTOMETRY, MS, RESOLUTION, CHALLENGES, ELEMENTS, QUANTIFY
journal title
ANALYTICAL CHEMISTRY
Anal. Chem.
volume
88
issue
11
pages
5783 - 5789
Web of Science type
Article
Web of Science id
000377631000034
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
6.32 (2016)
JCR rank
4/76 (2016)
JCR quartile
1 (2016)
ISSN
0003-2700
DOI
10.1021/acs.analchem.6b00334
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7257293
handle
http://hdl.handle.net/1854/LU-7257293
date created
2016-06-17 09:12:02
date last changed
2018-01-29 12:18:45
@article{7257293,
  abstract     = {This manuscript describes the development and characterization of a high-density microarray calibration standard, manufactured in-house and designed to overcome the limitations in precision, accuracy, and throughput of current calibration approaches for the quantification of elemental concentrations on the cellular level using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICPMS). As a case study, the accumulation of Cu in the model organism Scrippsiella trochoidea resulting from transition metal exposure (ranging from 0.5 to 100 \ensuremath{\mu}g/L) was evaluated. After the Cu exposure, cells of this photosynthetic dinoflagellate were treated with a critical point drying protocol, transferred to a carbon stub, and sputter-coated with a Au layer for scanning electron microscopy (SEM) analysis. In subsequent LA-ICPMS analysis, approximately 100 cells of each population were individually ablated. This approach permitted the evaluation of the mean concentration of Cu in the cell population across different exposure levels and also allowed the examination of the cellular distribution of Cu within the populations. In a cross-validation exercise, subcellular LA-ICPMS imaging was demonstrated to corroborate synchrotron radiation confocal X-ray fluorescence (SR-XRF) microimaging of single cells investigated under in vivo conditions.},
  author       = {Van Malderen, Stijn and Vergucht, Eva and De Rijcke, Maarten and Janssen, Colin and Vincze, Laszlo and Vanhaecke, Frank},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {OPTICAL TWEEZERS,LIQUID SAMPLES,MODEL ORGANISMS,TISSUE-SECTIONS,CYTOMETRY,MS,RESOLUTION,CHALLENGES,ELEMENTS,QUANTIFY},
  language     = {eng},
  number       = {11},
  pages        = {5783--5789},
  title        = {Quantitative determination and subcellular imaging of Cu in single cells via laser ablation-ICP-mass spectrometry using high-density microarray gelatin standards},
  url          = {http://dx.doi.org/10.1021/acs.analchem.6b00334},
  volume       = {88},
  year         = {2016},
}

Chicago
Van Malderen, Stijn, Eva Vergucht, Maarten De Rijcke, Colin Janssen, Laszlo Vincze, and Frank Vanhaecke. 2016. “Quantitative Determination and Subcellular Imaging of Cu in Single Cells via Laser ablation-ICP-mass Spectrometry Using High-density Microarray Gelatin Standards.” Analytical Chemistry 88 (11): 5783–5789.
APA
Van Malderen, Stijn, Vergucht, E., De Rijcke, M., Janssen, C., Vincze, L., & Vanhaecke, F. (2016). Quantitative determination and subcellular imaging of Cu in single cells via laser ablation-ICP-mass spectrometry using high-density microarray gelatin standards. ANALYTICAL CHEMISTRY, 88(11), 5783–5789.
Vancouver
1.
Van Malderen S, Vergucht E, De Rijcke M, Janssen C, Vincze L, Vanhaecke F. Quantitative determination and subcellular imaging of Cu in single cells via laser ablation-ICP-mass spectrometry using high-density microarray gelatin standards. ANALYTICAL CHEMISTRY. 2016;88(11):5783–9.
MLA
Van Malderen, Stijn, Eva Vergucht, Maarten De Rijcke, et al. “Quantitative Determination and Subcellular Imaging of Cu in Single Cells via Laser ablation-ICP-mass Spectrometry Using High-density Microarray Gelatin Standards.” ANALYTICAL CHEMISTRY 88.11 (2016): 5783–5789. Print.