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Molecular mechanisms underlying the close association between soil Burkholderia and fungi

Nejc Stopnisek, Daniela Zühlke, Aurélien Carlier UGent, Albert Barberán, Noah Fierer, Dörte Becher, Katharina Riedel, Leo Eberl and Laure Weisskopf (2016) ISME JOURNAL. 10(1). p.253-264
abstract
Bacterial species belonging to the genus Burkholderia have been repeatedly reported to be associated with fungi but the extent and specificity of these associations in soils remain undetermined. To assess whether associations between Burkholderia and fungi are widespread in soils, we performed a co-occurrence analysis in an intercontinental soil sample collection. This revealed that Burkholderia significantly co-occurred with a wide range of fungi. To analyse the molecular basis of the interaction, we selected two model fungi frequently co-occurring with Burkholderia, Alternaria alternata and Fusarium solani, and analysed the proteome changes caused by cultivation with either fungus in the widespread soil inhabitant B. glathei, whose genome we sequenced. Co-cultivation with both fungi led to very similar changes in the B. glathei proteome. Our results indicate that B. glathei significantly benefits from the interaction, which is exemplified by a lower abundance of several starvation factors that were highly expressed in pure culture. However, co-cultivation also gave rise to stress factors, as indicated by the increased expression of multidrug efflux pumps and proteins involved in oxidative stress response. Our data suggest that the ability of Burkholderia to establish a close association with fungi mainly lies in the capacities to utilize fungal-secreted metabolites and to overcome fungal defense mechanisms. This work indicates that beneficial interactions with fungi might contribute to the survival strategy of Burkholderia species in environments with sub-optimal conditions, including acidic soils.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SP STRAIN KARSTEN, RIBOSOMAL-RNA GENES, ESCHERICHIA-COLI, GENUS BURKHOLDERIA, TRANSPORT-SYSTEM, SP-NOV, BACTERIA, PROTEIN, CEPACIA, ANTIFUNGAL
journal title
ISME JOURNAL
ISME J.
volume
10
issue
1
pages
253 - 264
Web of Science type
Article
Web of Science id
000366671300023
JCR category
ECOLOGY
JCR impact factor
9.664 (2016)
JCR rank
3/153 (2016)
JCR quartile
1 (2016)
ISSN
1751-7362
DOI
10.1038/ismej.2015.73
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7257261
handle
http://hdl.handle.net/1854/LU-7257261
date created
2016-06-17 09:10:13
date last changed
2016-12-19 15:47:12
@article{7257261,
  abstract     = {Bacterial species belonging to the genus Burkholderia have been repeatedly reported to be associated with fungi but the extent and specificity of these associations in soils remain undetermined. To assess whether associations between Burkholderia and fungi are widespread in soils, we performed a co-occurrence analysis in an intercontinental soil sample collection. This revealed that Burkholderia significantly co-occurred with a wide range of fungi. To analyse the molecular basis of the interaction, we selected two model fungi frequently co-occurring with Burkholderia, Alternaria alternata and Fusarium solani, and analysed the proteome changes caused by cultivation with either fungus in the widespread soil inhabitant B. glathei, whose genome we sequenced. Co-cultivation with both fungi led to very similar changes in the B. glathei proteome. Our results indicate that B. glathei significantly benefits from the interaction, which is exemplified by a lower abundance of several starvation factors that were highly expressed in pure culture. However, co-cultivation also gave rise to stress factors, as indicated by the increased expression of multidrug efflux pumps and proteins involved in oxidative stress response. Our data suggest that the ability of Burkholderia to establish a close association with fungi mainly lies in the capacities to utilize fungal-secreted metabolites and to overcome fungal defense mechanisms. This work indicates that beneficial interactions with fungi might contribute to the survival strategy of Burkholderia species in environments with sub-optimal conditions, including acidic soils.},
  author       = {Stopnisek, Nejc and Z{\"u}hlke, Daniela and Carlier, Aur{\'e}lien and Barber{\'a}n, Albert and Fierer, Noah and Becher, D{\"o}rte and Riedel, Katharina and Eberl, Leo and Weisskopf, Laure},
  issn         = {1751-7362},
  journal      = {ISME JOURNAL},
  keyword      = {SP STRAIN KARSTEN,RIBOSOMAL-RNA GENES,ESCHERICHIA-COLI,GENUS BURKHOLDERIA,TRANSPORT-SYSTEM,SP-NOV,BACTERIA,PROTEIN,CEPACIA,ANTIFUNGAL},
  language     = {eng},
  number       = {1},
  pages        = {253--264},
  title        = {Molecular mechanisms underlying the close association between soil Burkholderia and fungi},
  url          = {http://dx.doi.org/10.1038/ismej.2015.73},
  volume       = {10},
  year         = {2016},
}

Chicago
Stopnisek, Nejc, Daniela Zühlke, Aurélien Carlier, Albert Barberán, Noah Fierer, Dörte Becher, Katharina Riedel, Leo Eberl, and Laure Weisskopf. 2016. “Molecular Mechanisms Underlying the Close Association Between Soil Burkholderia and Fungi.” Isme Journal 10 (1): 253–264.
APA
Stopnisek, N., Zühlke, D., Carlier, A., Barberán, A., Fierer, N., Becher, D., Riedel, K., et al. (2016). Molecular mechanisms underlying the close association between soil Burkholderia and fungi. ISME JOURNAL, 10(1), 253–264.
Vancouver
1.
Stopnisek N, Zühlke D, Carlier A, Barberán A, Fierer N, Becher D, et al. Molecular mechanisms underlying the close association between soil Burkholderia and fungi. ISME JOURNAL. 2016;10(1):253–64.
MLA
Stopnisek, Nejc, Daniela Zühlke, Aurélien Carlier, et al. “Molecular Mechanisms Underlying the Close Association Between Soil Burkholderia and Fungi.” ISME JOURNAL 10.1 (2016): 253–264. Print.