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Estrogen receptor α regulates β-cell formation during pancreas development and following injury

(2015) DIABETES. 64(9). p.3218-3228
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Abstract
Identifying pathways for beta-cell generation is essential for cell therapy in diabetes. We investigated the potential of 17 beta-estradiol (E-2) and estrogen receptor (ER) signaling for stimulating beta-cell generation during embryonic development and in the severely injured adult pancreas. E-2 concentration, ER activity, and number of ER alpha transcripts were enhanced in the pancreas injured by partial duct ligation (PDL) along with nuclear localization of ER alpha in beta-cells. PDL-induced proliferation of beta-cells depended on aromatase activity. The activation of Neurogenin3 (Ngn3) gene expression and beta-cell growth in PDL pancreas were impaired when ER was turned off chemically or genetically (ER alpha(-/-)), whereas in situ delivery of E-2 promoted beta-cell formation. In the embryonic pancreas, beta-cell replication, number of Ngn3(+) progenitor cells, and expression of key transcription factors of the endocrine lineage were decreased by ER alpha inactivation. The current study reveals that E-2 and ER alpha signaling can drive beta-cell replication and formation in mouse pancreas.
Keywords
MESSENGER-RNA EXPRESSION, ADULT-MOUSE PANCREAS, ER-ALPHA, MOLECULAR MECHANISMS, PROGENITOR CELLS, DUCT LIGATION, UP-REGULATION, PROLIFERATION, 17-BETA-ESTRADIOL, DIFFERENTIATION

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MLA
Yuchi, Yixing et al. “Estrogen Receptor α Regulates Β-cell Formation During Pancreas Development and Following Injury.” DIABETES 64.9 (2015): 3218–3228. Print.
APA
Yuchi, Y., Cai, Y., Legein, B., De Groef, S., Leuckx, G., Coppens, V., Van Overmeire, E., et al. (2015). Estrogen receptor α regulates β-cell formation during pancreas development and following injury. DIABETES, 64(9), 3218–3228.
Chicago author-date
Yuchi, Yixing, Ying Cai, Bart Legein, Sofie De Groef, Gunter Leuckx, Violette Coppens, Eva Van Overmeire, et al. 2015. “Estrogen Receptor α Regulates Β-cell Formation During Pancreas Development and Following Injury.” Diabetes 64 (9): 3218–3228.
Chicago author-date (all authors)
Yuchi, Yixing, Ying Cai, Bart Legein, Sofie De Groef, Gunter Leuckx, Violette Coppens, Eva Van Overmeire, Willem Staels, Nico De Leu, Geert Martens, Jo A Van Ginderachter, Harry Heimberg, and Mark Van de Casteele. 2015. “Estrogen Receptor α Regulates Β-cell Formation During Pancreas Development and Following Injury.” Diabetes 64 (9): 3218–3228.
Vancouver
1.
Yuchi Y, Cai Y, Legein B, De Groef S, Leuckx G, Coppens V, et al. Estrogen receptor α regulates β-cell formation during pancreas development and following injury. DIABETES. 2015;64(9):3218–28.
IEEE
[1]
Y. Yuchi et al., “Estrogen receptor α regulates β-cell formation during pancreas development and following injury,” DIABETES, vol. 64, no. 9, pp. 3218–3228, 2015.
@article{7256925,
  abstract     = {Identifying pathways for beta-cell generation is essential for cell therapy in diabetes. We investigated the potential of 17 beta-estradiol (E-2) and estrogen receptor (ER) signaling for stimulating beta-cell generation during embryonic development and in the severely injured adult pancreas. E-2 concentration, ER activity, and number of ER alpha transcripts were enhanced in the pancreas injured by partial duct ligation (PDL) along with nuclear localization of ER alpha in beta-cells. PDL-induced proliferation of beta-cells depended on aromatase activity. The activation of Neurogenin3 (Ngn3) gene expression and beta-cell growth in PDL pancreas were impaired when ER was turned off chemically or genetically (ER alpha(-/-)), whereas in situ delivery of E-2 promoted beta-cell formation. In the embryonic pancreas, beta-cell replication, number of Ngn3(+) progenitor cells, and expression of key transcription factors of the endocrine lineage were decreased by ER alpha inactivation. The current study reveals that E-2 and ER alpha signaling can drive beta-cell replication and formation in mouse pancreas.},
  author       = {Yuchi, Yixing and Cai, Ying and Legein, Bart and De Groef, Sofie and Leuckx, Gunter and Coppens, Violette and Van Overmeire, Eva and Staels, Willem and De Leu, Nico and Martens, Geert and Van Ginderachter, Jo A and Heimberg, Harry and Van de Casteele, Mark},
  issn         = {0012-1797},
  journal      = {DIABETES},
  keywords     = {MESSENGER-RNA EXPRESSION,ADULT-MOUSE PANCREAS,ER-ALPHA,MOLECULAR MECHANISMS,PROGENITOR CELLS,DUCT LIGATION,UP-REGULATION,PROLIFERATION,17-BETA-ESTRADIOL,DIFFERENTIATION},
  language     = {eng},
  number       = {9},
  pages        = {3218--3228},
  title        = {Estrogen receptor α regulates β-cell formation during pancreas development and following injury},
  url          = {http://dx.doi.org/10.2337/db14-1798},
  volume       = {64},
  year         = {2015},
}

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