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A review of COFRADIC techniques targeting protein N-terminal acetylation

Petra Van Damme UGent, Jozef Van Damme UGent, Hans Demol UGent, An Staes UGent, Joël Vandekerckhove UGent and Kris Gevaert UGent (2009) BMC PROCEEDINGS. 3(suppl. 6).
abstract
Acetylation of nascent protein Nalpha-termini is a common modification among archae and eukaryotes and can influence the structure and function of target proteins. This modification has been studied on an individual protein or (synthetic) peptide level or on a proteome scale using two-dimensional polyacrylamide gel electrophoresis. We recently developed mass spectrometry driven proteome analytical approaches specifically targeting the amino (N) terminus of proteins based on the concept of diagonal reverse-phase chromatography. We here review how this so-called combined fractional diagonal chromatography (COFRADIC) technique can be used in combination with differential mass-tagging strategies as to both qualitatively and quantitatively assess protein Nalpha-acetylation in whole proteomes.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
BMC PROCEEDINGS
BMC Proc.
volume
3
issue
suppl. 6
issue title
Proceedings of the 2007 and 2008 symposia on protein N-terminal acetylation
article_number
S6
pages
6 pages
conference name
NAT 2007 and 2008 Symposia : Protein N-terminal acetylation and protein N-terminal acetyltransferases (NATs)
conference location
Bergen, Norway
conference start
2009-05-24
conference end
2009-05-25
ISSN
1753-6561
DOI
10.1186/1753-6561-3-S6-S6
language
English
UGent publication?
yes
classification
C1
additional info
The NAT 2007 and 2008 symposia were held in Bergen, Norway on 24-25 May 2007 and 11-13 September 2008
copyright statement
I have retained and own the full copyright for this publication
id
725501
handle
http://hdl.handle.net/1854/LU-725501
date created
2009-08-11 15:27:58
date last changed
2012-03-12 09:56:03
@inproceedings{725501,
  abstract     = {Acetylation of nascent protein Nalpha-termini is a common modification among archae and eukaryotes and can influence the structure and function of target proteins. This modification has been studied on an individual protein or (synthetic) peptide level or on a proteome scale using two-dimensional polyacrylamide gel electrophoresis. We recently developed mass spectrometry driven proteome analytical approaches specifically targeting the amino (N) terminus of proteins based on the concept of diagonal reverse-phase chromatography. We here review how this so-called combined fractional diagonal chromatography (COFRADIC) technique can be used in combination with differential mass-tagging strategies as to both qualitatively and quantitatively assess protein Nalpha-acetylation in whole proteomes.},
  articleno    = {S6},
  author       = {Van Damme, Petra and Van Damme, Jozef and Demol, Hans and Staes, An and Vandekerckhove, Jo{\"e}l and Gevaert, Kris},
  booktitle    = {BMC PROCEEDINGS},
  issn         = {1753-6561},
  language     = {eng},
  location     = {Bergen, Norway},
  number       = {suppl. 6},
  pages        = {6},
  title        = {A review of COFRADIC techniques targeting protein N-terminal acetylation},
  url          = {http://dx.doi.org/10.1186/1753-6561-3-S6-S6},
  volume       = {3},
  year         = {2009},
}

Chicago
Van Damme, Petra, Jozef Van Damme, Hans Demol, An Staes, Joël Vandekerckhove, and Kris Gevaert. 2009. “A Review of COFRADIC Techniques Targeting Protein N-terminal Acetylation.” In Bmc Proceedings. Vol. 3.
APA
Van Damme, Petra, Van Damme, J., Demol, H., Staes, A., Vandekerckhove, J., & Gevaert, K. (2009). A review of COFRADIC techniques targeting protein N-terminal acetylation. BMC PROCEEDINGS (Vol. 3). Presented at the NAT 2007 and 2008 Symposia : Protein N-terminal acetylation and protein N-terminal acetyltransferases (NATs).
Vancouver
1.
Van Damme P, Van Damme J, Demol H, Staes A, Vandekerckhove J, Gevaert K. A review of COFRADIC techniques targeting protein N-terminal acetylation. BMC PROCEEDINGS. 2009.
MLA
Van Damme, Petra, Jozef Van Damme, Hans Demol, et al. “A Review of COFRADIC Techniques Targeting Protein N-terminal Acetylation.” Bmc Proceedings. Vol. 3. 2009. Print.