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Characterization of proteinuria in dogue de Bordeaux dogs, a breed predisposed to a familial glomerulonephropathy : a retrospective study

(2015) PLOS ONE. 10(7).
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Abstract
Dogue de Bordeaux dog has been reported to be predisposed to a familial glomerulonephropathy that displays some morphological modifications reported in focal and segmental glomerulosclerosis. Prevalence of quantitatively abnormal renal proteinuria was recently reported to be 33% in this breed. The nature of the proteinuria was assessed by sodium dodecyl sulfate-agarose gel electrophoresis and determinations of urinary markers (urinary retinol-binding protein, urinary N-acetyl-beta-glucosaminidase, urinary albumin and urinary immunoglobulin G) on stored specimens. Diagnostic performances of sodium dodecyl sulfate-agarose gel electrophoresis to identify dogs with elevated urinary biomarkers were assessed. Samples from 102 adult Dogue de Bordeaux dogs (47 non-proteinuric [urine protein-to-creatinine ratio <= 0.2], 20 borderline-proteinuric [0.2 < urine protein-to-creatinine ratio <= 0.5] and 35 proteinuric dogs [urine protein-to-creatinine ratio > 0.5]) were used, of which 2 were suffering from familial glomerulonephropathy. The electrophoretic protein patterns, for all but one proteinuric dog, were indicative of a glomerular origin and, in all dogs, the urinary albumin concentration related to creatinine concentration and the urinary immunoglobulin G concentration related to creatinine concentration were above the upper limit of the reference interval established for the breed. Sensitivity and specificity of sodium dodecyl sulfate-agarose gel electrophoresis identifying dogs with elevated urinary albumin concentration were 94% and 92%, respectively, while diagnostic performance of sodium dodecyl sulfate-agarose gel electrophoresis in detecting dogs with elevated urinary immunoglobulin G concentration yielded sensitivity and specificity of 90% and 74%, respectively. These results suggest that all proteinuric and some borderline-proteinuric Dogue de Bordeaux dogs likely have underlying glomerular lesions and that sodium dodecyl sulfate-agarose gel electrophoresis and urinary markers might be useful to screen dogs with borderline-proteinuria. Additional investigations are warranted to assess if these findings are related to the familial glomerulonephropathy.
Keywords
GLOMERULAR-DISEASE, KIDNEY, URINARY PROTEIN, RENAL-DISEASE, CANINE URINE, RETINOL-BINDING-PROTEIN, FOCAL SEGMENTAL GLOMERULOSCLEROSIS, AGAROSE GEL-ELECTROPHORESIS, TO-CREATININE RATIO, REFERENCE INTERVALS

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Chicago
Lavoué, Rachel, Catherine Trumel, Pascale Smets, Jean-Pierre Braun, Luca Aresu, Sylvie Daminet, Didier Concordet, Florence Palanché, and Dominique Peeters. 2015. “Characterization of Proteinuria in Dogue De Bordeaux Dogs, a Breed Predisposed to a Familial Glomerulonephropathy : a Retrospective Study.” Plos One 10 (7).
APA
Lavoué, R., Trumel, C., Smets, P., Braun, J.-P., Aresu, L., Daminet, S., Concordet, D., et al. (2015). Characterization of proteinuria in dogue de Bordeaux dogs, a breed predisposed to a familial glomerulonephropathy : a retrospective study. PLOS ONE, 10(7).
Vancouver
1.
Lavoué R, Trumel C, Smets P, Braun J-P, Aresu L, Daminet S, et al. Characterization of proteinuria in dogue de Bordeaux dogs, a breed predisposed to a familial glomerulonephropathy : a retrospective study. PLOS ONE. 2015;10(7).
MLA
Lavoué, Rachel et al. “Characterization of Proteinuria in Dogue De Bordeaux Dogs, a Breed Predisposed to a Familial Glomerulonephropathy : a Retrospective Study.” PLOS ONE 10.7 (2015): n. pag. Print.
@article{7252336,
  abstract     = {Dogue de Bordeaux dog has been reported to be predisposed to a familial glomerulonephropathy that displays some morphological modifications reported in focal and segmental glomerulosclerosis. Prevalence of quantitatively abnormal renal proteinuria was recently reported to be 33% in this breed. The nature of the proteinuria was assessed by sodium dodecyl sulfate-agarose gel electrophoresis and determinations of urinary markers (urinary retinol-binding protein, urinary N-acetyl-beta-glucosaminidase, urinary albumin and urinary immunoglobulin G) on stored specimens. Diagnostic performances of sodium dodecyl sulfate-agarose gel electrophoresis to identify dogs with elevated urinary biomarkers were assessed. Samples from 102 adult Dogue de Bordeaux dogs (47 non-proteinuric [urine protein-to-creatinine ratio <= 0.2], 20 borderline-proteinuric [0.2 < urine protein-to-creatinine ratio <= 0.5] and 35 proteinuric dogs [urine protein-to-creatinine ratio > 0.5]) were used, of which 2 were suffering from familial glomerulonephropathy. The electrophoretic protein patterns, for all but one proteinuric dog, were indicative of a glomerular origin and, in all dogs, the urinary albumin concentration related to creatinine concentration and the urinary immunoglobulin G concentration related to creatinine concentration were above the upper limit of the reference interval established for the breed. Sensitivity and specificity of sodium dodecyl sulfate-agarose gel electrophoresis identifying dogs with elevated urinary albumin concentration were 94% and 92%, respectively, while diagnostic performance of sodium dodecyl sulfate-agarose gel electrophoresis in detecting dogs with elevated urinary immunoglobulin G concentration yielded sensitivity and specificity of 90% and 74%, respectively. These results suggest that all proteinuric and some borderline-proteinuric Dogue de Bordeaux dogs likely have underlying glomerular lesions and that sodium dodecyl sulfate-agarose gel electrophoresis and urinary markers might be useful to screen dogs with borderline-proteinuria. Additional investigations are warranted to assess if these findings are related to the familial glomerulonephropathy.},
  articleno    = {e0133311},
  author       = {Lavoué, Rachel and Trumel, Catherine and Smets, Pascale and Braun, Jean-Pierre and Aresu, Luca and Daminet, Sylvie and Concordet, Didier and Palanché, Florence and Peeters, Dominique},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {GLOMERULAR-DISEASE,KIDNEY,URINARY PROTEIN,RENAL-DISEASE,CANINE URINE,RETINOL-BINDING-PROTEIN,FOCAL SEGMENTAL GLOMERULOSCLEROSIS,AGAROSE GEL-ELECTROPHORESIS,TO-CREATININE RATIO,REFERENCE INTERVALS},
  language     = {eng},
  number       = {7},
  title        = {Characterization of proteinuria in dogue de Bordeaux dogs, a breed predisposed to a familial glomerulonephropathy : a retrospective study},
  url          = {http://dx.doi.org/10.1371/journal.pone.0133311},
  volume       = {10},
  year         = {2015},
}

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