Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies
- Author
- Michael Schotsaert, Tine Ysenbaert (UGent) , Anouk Smet (UGent) , Bert Schepens (UGent) , Dieter Vanderschaeghe, Svetlana Stegalkina, Thorsten U Vogel, Nico Callewaert (UGent) , Walter Fiers and Xavier Saelens (UGent)
- Organization
- Abstract
- There is mounting evidence that in the absence of neutralizing antibodies cross-reactive T cells provide protection against pandemic influenza viruses. Here, we compared protection and CD8+ T cell responses following challenge with H1N1 2009 pandemic and H3N2 viruses of mice that had been immunized with hemagglutinin (HA), neuraminidase (NA) and the extracellular domain of matrix protein 2 (M2e) fused to a virus-like particle (VLP). Mice were challenged a first time with a sublethal dose of H1N1 2009 pandemic virus and, four weeks later, challenged again with an H3N2 virus. Mice that had been vaccinated with HA, NA, NA + M2e-VLP and HA + NA + M2e-VLP were protected against homologous H1N1 virus challenge. Challenged NA and NA + M2e-VLP vaccinated mice mounted CD8+ T cell responses that correlated with protection against secondary H3N2 challenge. HA-vaccinated mice were fully protected against challenge with homologous H1N1 2009 virus, failed to mount cross-reactive CD8+ T cells and succumbed to the second challenge with heterologous H3N2 virus. In summary, NA- and M2e-based immunity can protect against challenge with (homologous) virus without compromising the induction of robust cross-reactive CD8+ T cell responses upon exposure to virus.
- Keywords
- A/H5N1 VIRUS, MICE, A/H3N2 VIRUS, VIRAL NEURAMINIDASE, PANDEMIC H1N1, HETEROSUBTYPIC IMMUNITY, LETHAL INFECTION, VIRUS-INFECTION, T-CELL IMMUNITY, LYMPHOID-TISSUE IBALT
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-7250786
- MLA
- Schotsaert, Michael, et al. “Long-Lasting Cross-Protection against Influenza A by Neuraminidase and M2e-Based Immunization Strategies.” SCIENTIFIC REPORTS, vol. 6, 2016, doi:10.1038/srep24402.
- APA
- Schotsaert, M., Ysenbaert, T., Smet, A., Schepens, B., Vanderschaeghe, D., Stegalkina, S., … Saelens, X. (2016). Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies. SCIENTIFIC REPORTS, 6. https://doi.org/10.1038/srep24402
- Chicago author-date
- Schotsaert, Michael, Tine Ysenbaert, Anouk Smet, Bert Schepens, Dieter Vanderschaeghe, Svetlana Stegalkina, Thorsten U Vogel, Nico Callewaert, Walter Fiers, and Xavier Saelens. 2016. “Long-Lasting Cross-Protection against Influenza A by Neuraminidase and M2e-Based Immunization Strategies.” SCIENTIFIC REPORTS 6. https://doi.org/10.1038/srep24402.
- Chicago author-date (all authors)
- Schotsaert, Michael, Tine Ysenbaert, Anouk Smet, Bert Schepens, Dieter Vanderschaeghe, Svetlana Stegalkina, Thorsten U Vogel, Nico Callewaert, Walter Fiers, and Xavier Saelens. 2016. “Long-Lasting Cross-Protection against Influenza A by Neuraminidase and M2e-Based Immunization Strategies.” SCIENTIFIC REPORTS 6. doi:10.1038/srep24402.
- Vancouver
- 1.Schotsaert M, Ysenbaert T, Smet A, Schepens B, Vanderschaeghe D, Stegalkina S, et al. Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies. SCIENTIFIC REPORTS. 2016;6.
- IEEE
- [1]M. Schotsaert et al., “Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies,” SCIENTIFIC REPORTS, vol. 6, 2016.
@article{7250786, abstract = {{There is mounting evidence that in the absence of neutralizing antibodies cross-reactive T cells provide protection against pandemic influenza viruses. Here, we compared protection and CD8+ T cell responses following challenge with H1N1 2009 pandemic and H3N2 viruses of mice that had been immunized with hemagglutinin (HA), neuraminidase (NA) and the extracellular domain of matrix protein 2 (M2e) fused to a virus-like particle (VLP). Mice were challenged a first time with a sublethal dose of H1N1 2009 pandemic virus and, four weeks later, challenged again with an H3N2 virus. Mice that had been vaccinated with HA, NA, NA + M2e-VLP and HA + NA + M2e-VLP were protected against homologous H1N1 virus challenge. Challenged NA and NA + M2e-VLP vaccinated mice mounted CD8+ T cell responses that correlated with protection against secondary H3N2 challenge. HA-vaccinated mice were fully protected against challenge with homologous H1N1 2009 virus, failed to mount cross-reactive CD8+ T cells and succumbed to the second challenge with heterologous H3N2 virus. In summary, NA- and M2e-based immunity can protect against challenge with (homologous) virus without compromising the induction of robust cross-reactive CD8+ T cell responses upon exposure to virus.}}, articleno = {{24402}}, author = {{Schotsaert, Michael and Ysenbaert, Tine and Smet, Anouk and Schepens, Bert and Vanderschaeghe, Dieter and Stegalkina, Svetlana and Vogel, Thorsten U and Callewaert, Nico and Fiers, Walter and Saelens, Xavier}}, issn = {{2045-2322}}, journal = {{SCIENTIFIC REPORTS}}, keywords = {{A/H5N1 VIRUS,MICE,A/H3N2 VIRUS,VIRAL NEURAMINIDASE,PANDEMIC H1N1,HETEROSUBTYPIC IMMUNITY,LETHAL INFECTION,VIRUS-INFECTION,T-CELL IMMUNITY,LYMPHOID-TISSUE IBALT}}, language = {{eng}}, pages = {{22}}, title = {{Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies}}, url = {{http://doi.org/10.1038/srep24402}}, volume = {{6}}, year = {{2016}}, }
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