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Apibacter mensalis sp. nov. : a rare member of the bumblebee gut microbiota

Jessy Praet (UGent) , Maarten Aerts (UGent) , Evie De Brandt (UGent) , Ivan Meeus (UGent) , Guy Smagghe (UGent) and Peter Vandamme (UGent)
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Abstract
Isolates LMG 28357(T) (=R-53146(T)) and LMG 28623 were obtained from gut samples of Bombus lapidarius bumblebees caught in Ghent, Belgium. They had identical 16S rRNA gene sequences which were 95.7% identical to that of Apibacter adventoris wkB301(T), a member of the family Flavobacteriaceae. Both isolates had highly similar matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS and randomly amplified polymorphic DNA (RAPD) profiles. A draft genome sequence was obtained for strain LMG 28357(T) (Gold ID Gp0108260); its DNA G+C content was 30.4 %, which is within the range reported for members of the family Flavobacteriaceae (27 to 56 mol%) and which is similar to that of the type strain of A. adventoris (29.0 mol%). Whole-cell fatty acid methyl ester analysis of strain LMG 28357(T) revealed many branched-chain fatty acids, a typical characteristic of bacteria of the family Flavobacteriaceae and a profile that was similar to that reported for A. adventoris wkB301(T). MK6 was the major respiratory quinone, again conforming to bacteria of the family Flavobacteriaceae. The isolates LMG 28357(T) and LMG 28623 could be distinguished from A. adventoris strains through their oxidase activity. On the basis of phylogenetic, genotypic and phenotypic data, we propose to classify both isolates as representatives of a novel species of the genus Apibacter, Apibacter mensalis sp. nov., with LMG 28357(T) (=DSM 100903(T) =R-53146(T)) as the type strain.
Keywords
DECLINE, DIGESTIVE-TRACT, EXTRACTION, BEES, PROKARYOTES, GEN. NOV., PARASITE, DNA

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MLA
Praet, Jessy et al. “Apibacter Mensalis Sp. Nov. : a Rare Member of the Bumblebee Gut Microbiota.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66.4 (2016): 1645–1651. Print.
APA
Praet, J., Aerts, M., De Brandt, E., Meeus, I., Smagghe, G., & Vandamme, P. (2016). Apibacter mensalis sp. nov. : a rare member of the bumblebee gut microbiota. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 66(4), 1645–1651.
Chicago author-date
Praet, Jessy, Maarten Aerts, Evie De Brandt, Ivan Meeus, Guy Smagghe, and Peter Vandamme. 2016. “Apibacter Mensalis Sp. Nov. : a Rare Member of the Bumblebee Gut Microbiota.” International Journal of Systematic and Evolutionary Microbiology 66 (4): 1645–1651.
Chicago author-date (all authors)
Praet, Jessy, Maarten Aerts, Evie De Brandt, Ivan Meeus, Guy Smagghe, and Peter Vandamme. 2016. “Apibacter Mensalis Sp. Nov. : a Rare Member of the Bumblebee Gut Microbiota.” International Journal of Systematic and Evolutionary Microbiology 66 (4): 1645–1651.
Vancouver
1.
Praet J, Aerts M, De Brandt E, Meeus I, Smagghe G, Vandamme P. Apibacter mensalis sp. nov. : a rare member of the bumblebee gut microbiota. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2016;66(4):1645–51.
IEEE
[1]
J. Praet, M. Aerts, E. De Brandt, I. Meeus, G. Smagghe, and P. Vandamme, “Apibacter mensalis sp. nov. : a rare member of the bumblebee gut microbiota,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 66, no. 4, pp. 1645–1651, 2016.
@article{7250371,
  abstract     = {{Isolates LMG 28357(T) (=R-53146(T)) and LMG 28623 were obtained from gut samples of Bombus lapidarius bumblebees caught in Ghent, Belgium. They had identical 16S rRNA gene sequences which were 95.7% identical to that of Apibacter adventoris wkB301(T), a member of the family Flavobacteriaceae. Both isolates had highly similar matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS and randomly amplified polymorphic DNA (RAPD) profiles. A draft genome sequence was obtained for strain LMG 28357(T) (Gold ID Gp0108260); its DNA G+C content was 30.4 %, which is within the range reported for members of the family Flavobacteriaceae (27 to 56 mol%) and which is similar to that of the type strain of A. adventoris (29.0 mol%). Whole-cell fatty acid methyl ester analysis of strain LMG 28357(T) revealed many branched-chain fatty acids, a typical characteristic of bacteria of the family Flavobacteriaceae and a profile that was similar to that reported for A. adventoris wkB301(T). MK6 was the major respiratory quinone, again conforming to bacteria of the family Flavobacteriaceae. The isolates LMG 28357(T) and LMG 28623 could be distinguished from A. adventoris strains through their oxidase activity. On the basis of phylogenetic, genotypic and phenotypic data, we propose to classify both isolates as representatives of a novel species of the genus Apibacter, Apibacter mensalis sp. nov., with LMG 28357(T) (=DSM 100903(T) =R-53146(T)) as the type strain.}},
  author       = {{Praet, Jessy and Aerts, Maarten and De Brandt, Evie and Meeus, Ivan and Smagghe, Guy and Vandamme, Peter}},
  issn         = {{1466-5026}},
  journal      = {{INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}},
  keywords     = {{DECLINE,DIGESTIVE-TRACT,EXTRACTION,BEES,PROKARYOTES,GEN. NOV.,PARASITE,DNA}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1645--1651}},
  title        = {{Apibacter mensalis sp. nov. : a rare member of the bumblebee gut microbiota}},
  url          = {{http://dx.doi.org/10.1099/ijsem.0.000921}},
  volume       = {{66}},
  year         = {{2016}},
}

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