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Daphnia magna transcriptome by RNA-Seq across 12 environmental stressors

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Abstract
The full exploration of gene-environment interactions requires model organisms with well-characterized ecological interactions in their natural environment, manipulability in the laboratory and genomic tools. The waterflea Daphnia magna is an established ecological and toxicological model species, central to the foodwebs of freshwater lentic habitats and sentinel for water quality. Its tractability and cyclic parthenogenetic life-cycle are ideal to investigate links between genes and the environment. Capitalizing on this unique model system, the STRESSFLEA consortium generated a comprehensive RNA-Seq data set by exposing two inbred genotypes of D. magna and a recombinant cross of these genotypes to a range of environmental perturbations. Gene models were constructed from the transcriptome data and mapped onto the draft genome of D. magna using EvidentialGene. The transcriptome data generated here, together with the available draft genome sequence of D. magna and a high-density genetic map will be a key asset for future investigations in environmental genomics.

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Please use this url to cite or link to this publication:

MLA
Orsini, Luisa, Donald Gilbert, Ram Podicheti, et al. “Daphnia Magna Transcriptome by RNA-Seq Across 12 Environmental Stressors.” SCIENTIFIC DATA 3 (2016): n. pag. Print.
APA
Orsini, Luisa, Gilbert, D., Podicheti, R., Jansen, M., Brown, J. B., Solari, O. S., Spanier, K. I., et al. (2016). Daphnia magna transcriptome by RNA-Seq across 12 environmental stressors. SCIENTIFIC DATA, 3.
Chicago author-date
Orsini, Luisa, Donald Gilbert, Ram Podicheti, Mieke Jansen, James B Brown, Omid Shams Solari, Katina I Spanier, et al. 2016. “Daphnia Magna Transcriptome by RNA-Seq Across 12 Environmental Stressors.” Scientific Data 3.
Chicago author-date (all authors)
Orsini, Luisa, Donald Gilbert, Ram Podicheti, Mieke Jansen, James B Brown, Omid Shams Solari, Katina I Spanier, John K Colbourne, Douglas Rush, Ellen Decaestecker, Jana Asselman, Karel De Schamphelaere, Dieter Ebert, Christoph R Haag, Jouni Kvist, Christian Laforsch, Adam Petrusek, Andrew P Beckerman, Tom J Little, Anurag Chaturvedi, Michael E Pfrender, Luc De Meester, and Mikko J Frilander. 2016. “Daphnia Magna Transcriptome by RNA-Seq Across 12 Environmental Stressors.” Scientific Data 3.
Vancouver
1.
Orsini L, Gilbert D, Podicheti R, Jansen M, Brown JB, Solari OS, et al. Daphnia magna transcriptome by RNA-Seq across 12 environmental stressors. SCIENTIFIC DATA. 2016;3.
IEEE
[1]
L. Orsini et al., “Daphnia magna transcriptome by RNA-Seq across 12 environmental stressors,” SCIENTIFIC DATA, vol. 3, 2016.
@article{7248070,
  abstract     = {The full exploration of gene-environment  interactions requires model organisms with well-characterized ecological interactions in their natural environment, manipulability in the laboratory and genomic tools. The waterflea Daphnia  magna is an established ecological and toxicological model species, central to the foodwebs of freshwater lentic habitats and sentinel for water quality. Its tractability and cyclic parthenogenetic life-cycle are ideal to investigate links between genes and the environment. Capitalizing on this unique model system, the STRESSFLEA consortium generated a comprehensive RNA-Seq data set by exposing two inbred genotypes of D. magna and a recombinant cross of these genotypes to a range of environmental perturbations. Gene models were constructed from the transcriptome data and mapped onto the draft genome of D.  magna using EvidentialGene. The transcriptome data generated here, together with the available draft genome sequence of D. magna and a high-density genetic map will be a key asset for future investigations in environmental genomics.},
  articleno    = {160030},
  author       = {Orsini, Luisa and Gilbert, Donald and Podicheti, Ram and Jansen, Mieke and Brown, James B and Solari, Omid Shams and Spanier, Katina I and Colbourne, John K and Rush, Douglas and Decaestecker, Ellen and Asselman, Jana and De Schamphelaere, Karel and Ebert, Dieter and Haag, Christoph R and Kvist, Jouni and Laforsch, Christian and Petrusek, Adam and Beckerman, Andrew P and Little, Tom J and Chaturvedi, Anurag and Pfrender, Michael E and De Meester, Luc and Frilander, Mikko J},
  issn         = {2052-4463},
  journal      = {SCIENTIFIC DATA},
  language     = {eng},
  pages        = {15},
  title        = {Daphnia magna transcriptome by RNA-Seq across 12 environmental stressors},
  url          = {http://dx.doi.org/10.1038/sdata.2016.30},
  volume       = {3},
  year         = {2016},
}

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