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Housekeeping gene selection for real time-PCR normalization in female hop (Humulus lupulus L) tissues

Lina Maloukh UGent, Jaroslav Matousek, Erik Van Bockstaele and Isabel Roldàn-Ruiz UGent (2009) JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY. 18(1). p.53-58
abstract
The variability of transcript accumulation of six genes encoding chloropyll-a/b (Chla/b) binding protein, Nicotinamide Adenine Dinucleotide Hydride (NADH) dehydrogenase, Histone H3 (H3); DEAD-box RNA helicase 1 (DRH1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and of the 7SL component of the. signal recognition particle (7SL-RNA), was estimated in leaves and female inflorescences of the two hop cultivars, White Golding and Admiral at different developmental stages by RT-PCR. The value of these genes as internal, normalization controls in gene transcript accumulation studies was assessed. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript, accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS, XANTHOHUMOL, Hop, gene expression, Humulus lupulus, normalization, Real time-PCR, POLYMERASE-CHAIN-REACTION, RT-PCR, EXPRESSION, VALIDATION, RNA, QUANTIFICATION, BIOSYNTHESIS, PLANT
journal title
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
J. Plant Biochem. Biotechnol.
volume
18
issue
1
pages
53 - 58
Web of Science type
Article
Web of Science id
000263489400007
JCR category
PLANT SCIENCES
JCR impact factor
0.323 (2009)
JCR rank
157/172 (2009)
JCR quartile
4 (2009)
ISSN
0971-7811
language
English
UGent publication?
yes
classification
A1
id
724654
handle
http://hdl.handle.net/1854/LU-724654
date created
2009-08-11 08:12:04
date last changed
2016-12-19 15:44:45
@article{724654,
  abstract     = {The variability of transcript accumulation of six genes encoding chloropyll-a/b (Chla/b) binding protein, Nicotinamide Adenine Dinucleotide Hydride (NADH) dehydrogenase, Histone H3 (H3); DEAD-box RNA helicase 1 (DRH1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and of the 7SL component of the. signal recognition particle (7SL-RNA), was estimated in leaves and female inflorescences of the two hop cultivars, White Golding and Admiral at different developmental stages by RT-PCR. The value of these genes as internal, normalization controls in gene transcript accumulation studies was assessed. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript, accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.},
  author       = {Maloukh, Lina and Matousek, Jaroslav and Van Bockstaele, Erik and Rold{\`a}n-Ruiz, Isabel},
  issn         = {0971-7811},
  journal      = {JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY},
  keyword      = {ARABIDOPSIS,XANTHOHUMOL,Hop,gene expression,Humulus lupulus,normalization,Real time-PCR,POLYMERASE-CHAIN-REACTION,RT-PCR,EXPRESSION,VALIDATION,RNA,QUANTIFICATION,BIOSYNTHESIS,PLANT},
  language     = {eng},
  number       = {1},
  pages        = {53--58},
  title        = {Housekeeping gene selection for real time-PCR normalization in female hop (Humulus lupulus L) tissues},
  volume       = {18},
  year         = {2009},
}

Chicago
Maloukh, Lina, Jaroslav Matousek, Erik Van Bockstaele, and Isabel Roldàn-Ruiz. 2009. “Housekeeping Gene Selection for Real time-PCR Normalization in Female Hop (Humulus Lupulus L) Tissues.” Journal of Plant Biochemistry and Biotechnology 18 (1): 53–58.
APA
Maloukh, L., Matousek, J., Van Bockstaele, E., & Roldàn-Ruiz, I. (2009). Housekeeping gene selection for real time-PCR normalization in female hop (Humulus lupulus L) tissues. JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 18(1), 53–58.
Vancouver
1.
Maloukh L, Matousek J, Van Bockstaele E, Roldàn-Ruiz I. Housekeeping gene selection for real time-PCR normalization in female hop (Humulus lupulus L) tissues. JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY. 2009;18(1):53–8.
MLA
Maloukh, Lina, Jaroslav Matousek, Erik Van Bockstaele, et al. “Housekeeping Gene Selection for Real time-PCR Normalization in Female Hop (Humulus Lupulus L) Tissues.” JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 18.1 (2009): 53–58. Print.