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Fast and sensitive LC-MS/MS method measuring human mycotoxin exposure using biomarkers in urine

(2015) ARCHIVES OF TOXICOLOGY. 89(11). p.1993-2005
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Abstract
A direct, fast and sensitive LC-MS/MS method was developed to measure biomarkers for mycotoxin exposure in human urine. In total, 32 biomarkers were quantitatively or semi-quantitatively measured in 32 urine samples of Belgian volunteers using two injections. All urine samples contained deoxynivalenol-15-glucuronide, the major detoxification metabolite of deoxynivalenol, in the ng/mL range. Also deoxynivalenol-3-glucuronide and de-epoxy-deoxynivalenol-glucuronide were present in, respectively, 90 and 25 % of the samples, while deoxynivalenol was detected in 60 % of the samples, in lower concentrations. Deoxynivalenol glucuronides were the major biomarkers for deoxynivalenol exposure. Ochratoxin A was detected in 70 % of the samples in pg/mL. Citrinin and/or dihydrocitrinone were detected in 90 % of the samples, also in concentrations of pg/mL. The presence of ochratoxin A and citrinin was confirmed by a second method using sample cleanup by immunoaffinity columns, followed by LC-MS/MS. Our data show that humans are much more exposed to citrinin than realized before and suggest further work on citrinin exposure in relation with ochratoxin A exposure, as both mycotoxins are nephrotoxic.
Keywords
CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY, LC-MS/MS, Glucuronides, Mycotoxin exposure, Urine, Biomarkers, METABOLITE OCHRATOXIN ALPHA, HUMAN HEALTH, UK ADULTS, IN-VITRO, DEOXYNIVALENOL EXPOSURE, LIQUID-CHROMATOGRAPHY, LIVER-MICROSOMES, FOOD, ZEARALENONE

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Citation

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Chicago
Huybrechts, B, José Martins, Ph Debongnie, S Uhlig, and A Callebaut. 2015. “Fast and Sensitive LC-MS/MS Method Measuring Human Mycotoxin Exposure Using Biomarkers in Urine.” Archives of Toxicology 89 (11): 1993–2005.
APA
Huybrechts, B., Martins, J., Debongnie, P., Uhlig, S., & Callebaut, A. (2015). Fast and sensitive LC-MS/MS method measuring human mycotoxin exposure using biomarkers in urine. ARCHIVES OF TOXICOLOGY, 89(11), 1993–2005.
Vancouver
1.
Huybrechts B, Martins J, Debongnie P, Uhlig S, Callebaut A. Fast and sensitive LC-MS/MS method measuring human mycotoxin exposure using biomarkers in urine. ARCHIVES OF TOXICOLOGY. 2015;89(11):1993–2005.
MLA
Huybrechts, B, José Martins, Ph Debongnie, et al. “Fast and Sensitive LC-MS/MS Method Measuring Human Mycotoxin Exposure Using Biomarkers in Urine.” ARCHIVES OF TOXICOLOGY 89.11 (2015): 1993–2005. Print.
@article{7245274,
  abstract     = {A direct, fast and sensitive LC-MS/MS method was developed to measure biomarkers for mycotoxin exposure in human urine. In total, 32 biomarkers were quantitatively or semi-quantitatively measured in 32 urine samples of Belgian volunteers using two injections. All urine samples contained deoxynivalenol-15-glucuronide, the major detoxification metabolite of deoxynivalenol, in the ng/mL range. Also deoxynivalenol-3-glucuronide and de-epoxy-deoxynivalenol-glucuronide were present in, respectively, 90 and 25 \% of the samples, while deoxynivalenol was detected in 60 \% of the samples, in lower concentrations. Deoxynivalenol glucuronides were the major biomarkers for deoxynivalenol exposure. Ochratoxin A was detected in 70 \% of the samples in pg/mL. Citrinin and/or dihydrocitrinone were detected in 90 \% of the samples, also in concentrations of pg/mL. The presence of ochratoxin A and citrinin was confirmed by a second method using sample cleanup by immunoaffinity columns, followed by LC-MS/MS. Our data show that humans are much more exposed to citrinin than realized before and suggest further work on citrinin exposure in relation with ochratoxin A exposure, as both mycotoxins are nephrotoxic.},
  author       = {Huybrechts, B and Martins, Jos{\'e} and Debongnie, Ph and Uhlig, S and Callebaut, A},
  issn         = {0340-5761},
  journal      = {ARCHIVES OF TOXICOLOGY},
  keyword      = {CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY,LC-MS/MS,Glucuronides,Mycotoxin exposure,Urine,Biomarkers,METABOLITE OCHRATOXIN ALPHA,HUMAN HEALTH,UK ADULTS,IN-VITRO,DEOXYNIVALENOL EXPOSURE,LIQUID-CHROMATOGRAPHY,LIVER-MICROSOMES,FOOD,ZEARALENONE},
  language     = {eng},
  number       = {11},
  pages        = {1993--2005},
  title        = {Fast and sensitive LC-MS/MS method measuring human mycotoxin exposure using biomarkers in urine},
  url          = {http://dx.doi.org/10.1007/s00204-014-1358-8},
  volume       = {89},
  year         = {2015},
}

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