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Lutibacter holmesii sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Lutibacter

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Abstract
Seven Gram-staining-negative, strictly aerobic, pale-yellow-pigmented, rod-shaped and nonmotile strains were isolated from the sea urchin Strongylocentrotus intermedius collected from Troitsa Bay, Sea of Japan. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were affiliated with the family Flavobacteriaceae. The novel isolates showed 99.9-100 % 16S rRNA gene sequence similarity to each other and were closely related to the type strains of the recognized members of the genus Lutibacter with sequence similarities of 95.8-98.4 %. The G + C content of the genomic DNA was 35-36 mol%. DNA-DNA relatedness among the sea urchin isolates was 95-99 % and between strain KMM 6277(T) and its most closely related type strains, Lutibacter agarilyticus KCTC 23842(T) and Lutibacter litoralis JCM 13034(T), was 38 and 27 %, respectively. The prevalent fatty acids were iso-C-15 (: 0), anteiso-C-15 (: 0), summed feature 3 (comprising iso-C-15 : 0 2-OH and/or C-16 : 1 omega 7c fatty acids), iso-C-15 : 1 and C-15 : 0. The polar lipid profile was composed of the phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. The main respiratory isoprenoid quinone was MK-6. The results of phylogenetic, phenotypic and genotypic analyses indicated that the novel strains represent a novel species within the genus Lutibacter, for which the name Lutibacter holmesii sp. nov. is proposed. The type strain is KMM 6277(T) (= CCUG 62221(T) = LMG 26737(T)).
Keywords
TIDAL FLAT SEDIMENT, DEOXYRIBONUCLEIC-ACID, SYSTEMATICS, SEAWATER

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Chicago
Nedashkovskaya, Olga I, Stefanie Van Trappen, Natalia V Zhukova, and Paul De Vos. 2015. “Lutibacter Holmesii Sp. Nov., a Marine Bacterium of the Family Flavobacteriaceae Isolated from the Sea Urchin Strongylocentrotus Intermedius, and Emended Description of the Genus Lutibacter.” International Journal of Systematic and Evolutionary Microbiology 65 (11): 3991–3996.
APA
Nedashkovskaya, O. I., Van Trappen, S., Zhukova, N. V., & De Vos, P. (2015). Lutibacter holmesii sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Lutibacter. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 65(11), 3991–3996.
Vancouver
1.
Nedashkovskaya OI, Van Trappen S, Zhukova NV, De Vos P. Lutibacter holmesii sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Lutibacter. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2015;65(11):3991–6.
MLA
Nedashkovskaya, Olga I, Stefanie Van Trappen, Natalia V Zhukova, et al. “Lutibacter Holmesii Sp. Nov., a Marine Bacterium of the Family Flavobacteriaceae Isolated from the Sea Urchin Strongylocentrotus Intermedius, and Emended Description of the Genus Lutibacter.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65.11 (2015): 3991–3996. Print.
@article{7244641,
  abstract     = {Seven Gram-staining-negative, strictly aerobic, pale-yellow-pigmented, rod-shaped and nonmotile strains were isolated from the sea urchin Strongylocentrotus intermedius collected from Troitsa Bay, Sea of Japan. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were affiliated with the family Flavobacteriaceae. The novel isolates showed 99.9-100 \% 16S rRNA gene sequence similarity to each other and were closely related to the type strains of the recognized members of the genus Lutibacter with sequence similarities of 95.8-98.4 \%. The G + C content of the genomic DNA was 35-36 mol\%. DNA-DNA relatedness among the sea urchin isolates was 95-99 \% and between strain KMM 6277(T) and its most closely related type strains, Lutibacter agarilyticus KCTC 23842(T) and Lutibacter litoralis JCM 13034(T), was 38 and 27 \%, respectively. The prevalent fatty acids were iso-C-15 (: 0), anteiso-C-15 (: 0), summed feature 3 (comprising iso-C-15 : 0 2-OH and/or C-16 : 1 omega 7c fatty acids), iso-C-15 : 1 and C-15 : 0. The polar lipid profile was composed of the phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. The main respiratory isoprenoid quinone was MK-6. The results of phylogenetic, phenotypic and genotypic analyses indicated that the novel strains represent a novel species within the genus Lutibacter, for which the name Lutibacter holmesii sp. nov. is proposed. The type strain is KMM 6277(T) (= CCUG 62221(T) = LMG 26737(T)).},
  author       = {Nedashkovskaya, Olga I and Van Trappen, Stefanie and Zhukova, Natalia V and De Vos, Paul},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {TIDAL FLAT SEDIMENT,DEOXYRIBONUCLEIC-ACID,SYSTEMATICS,SEAWATER},
  language     = {eng},
  number       = {11},
  pages        = {3991--3996},
  title        = {Lutibacter holmesii sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Lutibacter},
  url          = {http://dx.doi.org/10.1099/ijsem.0.000525},
  volume       = {65},
  year         = {2015},
}

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