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Overexpression of two α-esterase genes mediates metabolic resistance to malathion in the oriental fruit fly, Bactrocera dorsalis (Hendel)

(2015) INSECT MOLECULAR BIOLOGY. 24(4). p.467-479
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Abstract
Esterase has been reported to be involved in malathion resistance in the oriental fruit fly, Bactrocera dorsalis (Hendel). However, the underlying molecular mechanism of the esterase-mediated resistance remains largely unknown in this species. Here, with the use of a strain selected for malathion resistance in the laboratory (MR), we found that two overexpressed -esterase genes, namely BdCarE4 and BdCarE6, predominant in the adult midgut and fat body, function in conferring malathion resistance in B.dorsalis. Notably, these two genes were found to be mostly close to the esterase E3, which are usually implicated in detoxifying organophosphate insecticides. The transcript levels of BdCarE4 and BdCarE6 were investigated and compared between the MR and a susceptible (MS) strain of B.dorsalis. Both genes were significantly up-regulated in the MR strain, which was consistent with the enhanced esterase activity in the MR strain. However, no changes in either the coding sequence or gene copy number were observed between the two strains. Subsequently, heterologous expression combined with cytotoxicity assay in Sf9 cells demonstrated that BdCarE4 and BdCarE6 can probably detoxify malathion. Furthermore, RNA interference-mediated knockdown of each of these two genes significantly increased malathion susceptibility in the MR strain adults. In conclusion, these results expand our molecular understanding of the important role of -esterases during the development of resistance to organophosphorous insecticides in B.dorsalis.
Keywords
-esterase, metabolic resistance, heterologous expression, cytotoxicity assay, RNA interference, AMINO-ACID SUBSTITUTION, INSECTICIDE RESISTANCE, LUCILIA-CUPRINA, ORGANOPHOSPHATE RESISTANCE, MOLECULAR CHARACTERIZATION, DIPTERA TEPHRITIDAE, SUSCEPTIBLE STRAINS, FIELD POPULATIONS, MYZUS-PERSICAE, CARBOXYLESTERASE

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Citation

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Chicago
Wang, L-L, Y Huang, X-P Lu, X-Z Jiang, Guy Smagghe, Z-J Feng, G-R Yuan, D Wei, and J-J Wang. 2015. “Overexpression of Two Α-esterase Genes Mediates Metabolic Resistance to Malathion in the Oriental Fruit Fly, Bactrocera Dorsalis (Hendel).” Insect Molecular Biology 24 (4): 467–479.
APA
Wang, L.-L., Huang, Y., Lu, X.-P., Jiang, X.-Z., Smagghe, G., Feng, Z.-J., Yuan, G.-R., et al. (2015). Overexpression of two α-esterase genes mediates metabolic resistance to malathion in the oriental fruit fly, Bactrocera dorsalis (Hendel). INSECT MOLECULAR BIOLOGY, 24(4), 467–479.
Vancouver
1.
Wang L-L, Huang Y, Lu X-P, Jiang X-Z, Smagghe G, Feng Z-J, et al. Overexpression of two α-esterase genes mediates metabolic resistance to malathion in the oriental fruit fly, Bactrocera dorsalis (Hendel). INSECT MOLECULAR BIOLOGY. 2015;24(4):467–79.
MLA
Wang, L-L, Y Huang, X-P Lu, et al. “Overexpression of Two Α-esterase Genes Mediates Metabolic Resistance to Malathion in the Oriental Fruit Fly, Bactrocera Dorsalis (Hendel).” INSECT MOLECULAR BIOLOGY 24.4 (2015): 467–479. Print.
@article{7239709,
  abstract     = {Esterase has been reported to be involved in malathion resistance in the oriental fruit fly, Bactrocera dorsalis (Hendel). However, the underlying molecular mechanism of the esterase-mediated resistance remains largely unknown in this species. Here, with the use of a strain selected for malathion resistance in the laboratory (MR), we found that two overexpressed -esterase genes, namely BdCarE4 and BdCarE6, predominant in the adult midgut and fat body, function in conferring malathion resistance in B.dorsalis. Notably, these two genes were found to be mostly close to the esterase E3, which are usually implicated in detoxifying organophosphate insecticides. The transcript levels of BdCarE4 and BdCarE6 were investigated and compared between the MR and a susceptible (MS) strain of B.dorsalis. Both genes were significantly up-regulated in the MR strain, which was consistent with the enhanced esterase activity in the MR strain. However, no changes in either the coding sequence or gene copy number were observed between the two strains. Subsequently, heterologous expression combined with cytotoxicity assay in Sf9 cells demonstrated that BdCarE4 and BdCarE6 can probably detoxify malathion. Furthermore, RNA interference-mediated knockdown of each of these two genes significantly increased malathion susceptibility in the MR strain adults. In conclusion, these results expand our molecular understanding of the important role of -esterases during the development of resistance to organophosphorous insecticides in B.dorsalis.},
  author       = {Wang, L-L and Huang, Y and Lu, X-P and Jiang, X-Z and Smagghe, Guy and Feng, Z-J and Yuan, G-R and Wei, D and Wang, J-J},
  issn         = {0962-1075},
  journal      = {INSECT MOLECULAR BIOLOGY},
  language     = {eng},
  number       = {4},
  pages        = {467--479},
  title        = {Overexpression of two \ensuremath{\alpha}-esterase genes mediates metabolic resistance to malathion in the oriental fruit fly, Bactrocera dorsalis (Hendel)},
  url          = {http://dx.doi.org/10.1111/imb.12173},
  volume       = {24},
  year         = {2015},
}

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