Ghent University Academic Bibliography

Advanced

Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis

Katrien Van Beneden UGent, Ken Coppieters UGent, W Laroy, Filip De Keyser UGent, Ilse Hoffman, Filip Van den Bosch UGent, BERT VANDER CRUYSSEN UGent, Michael Drennan UGent, PEGGY JACQUES UGent and P Rottiers, et al. (2009) ANNALS OF THE RHEUMATIC DISEASES. 68(8). p.1360-1365
abstract
Objectives: Improved DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) technology was used to monitor the changes in the galactosylation status of serum immunoglobulins during the immune response and therapy of autoimmune arthritis. Methods: Collagen-induced arthritis (CIA) was induced in susceptible DBA/1 mice and the undergalactosylation status (UGS) of serum immunoglobulins was determined using the improved DSA-FACE technology. Prophylactic intravenous tolerisation with type II collagen as well as semitherapeutic treatment with dexamethasone (DEX) were performed and UGS was analysed. Next, the serum immunoglobulin glycosylation profiles of patients with rheumatoid arthritis (RA) and spondyloarthropathy (SpA) were studied and changes in the UGS scores during antitumour necrosis factor (TNF)alpha therapy followed. Results: In the longitudinal CIA study, the undergalactosylation state of immunoglobulins was found to be significantly correlated with the clinical arthritis scores. Upon collagen-specific tolerisation as well as glucocorticoid semitherapeutic treatment, improvement of the clinical arthritis scores correlated with decreased levels of UGS. It was also demonstrated that withdrawal of DEX was associated with an increased UGS score. Interestingly, reversibility in the UGS was also shown during treatment of patients with RA and SpA with anti-TNF alpha. Conclusions: These findings demonstrate that the UGS of serum immunoglobulins changes during the disease course of CIA and that this UGS is inhibited by antigen-specific and antigen-independent treatment procedures. The observation that Ig galactosylation is a reversible process is also documented during treatment of patients with RA and SpA with anti-TNF alpha.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RHEUMATOID-ARTHRITIS, DNA-SEQUENCING EQUIPMENT, DIFFERENTIAL-DIAGNOSIS, AGALACTOSYL IGG, GLYCOFORMS, GLYCOSYLATION, DISEASE, COMPLEMENT, N-LINKED OLIGOSACCHARIDES, PREGNANCY
journal title
ANNALS OF THE RHEUMATIC DISEASES
Ann. Rheum. Dis.
volume
68
issue
8
pages
1360 - 1365
Web of Science type
Article
Web of Science id
000268010500021
JCR category
RHEUMATOLOGY
JCR impact factor
8.111 (2009)
JCR rank
1/24 (2009)
JCR quartile
1 (2009)
ISSN
0003-4967
DOI
10.1136/ard.2008.089292
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
723755
handle
http://hdl.handle.net/1854/LU-723755
date created
2009-08-07 13:54:55
date last changed
2012-06-26 14:31:45
@article{723755,
  abstract     = {Objectives: Improved DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) technology was used to monitor the changes in the galactosylation status of serum immunoglobulins during the immune response and therapy of autoimmune arthritis.
Methods: Collagen-induced arthritis (CIA) was induced in susceptible DBA/1 mice and the undergalactosylation status (UGS) of serum immunoglobulins was determined using the improved DSA-FACE technology. Prophylactic intravenous tolerisation with type II collagen as well as semitherapeutic treatment with dexamethasone (DEX) were performed and UGS was analysed. Next, the serum immunoglobulin glycosylation profiles of patients with rheumatoid arthritis (RA) and spondyloarthropathy (SpA) were studied and changes in the UGS scores during antitumour necrosis factor (TNF)alpha therapy followed.
Results: In the longitudinal CIA study, the undergalactosylation state of immunoglobulins was found to be significantly correlated with the clinical arthritis scores. Upon collagen-specific tolerisation as well as glucocorticoid semitherapeutic treatment, improvement of the clinical arthritis scores correlated with decreased levels of UGS. It was also demonstrated that withdrawal of DEX was associated with an increased UGS score. Interestingly, reversibility in the UGS was also shown during treatment of patients with RA and SpA with anti-TNF alpha.
Conclusions: These findings demonstrate that the UGS of serum immunoglobulins changes during the disease course of CIA and that this UGS is inhibited by antigen-specific and antigen-independent treatment procedures. The observation that Ig galactosylation is a reversible process is also documented during treatment of patients with RA and SpA with anti-TNF alpha.},
  author       = {Van Beneden, Katrien and Coppieters, Ken and Laroy, W and De Keyser, Filip and Hoffman, Ilse and Van den Bosch, Filip and VANDER CRUYSSEN, BERT and Drennan, Michael and JACQUES, PEGGY and Rottiers, P and Verbruggen, August and Contreras, Roland and Callewaert, Nico and Elewaut, Dirk},
  issn         = {0003-4967},
  journal      = {ANNALS OF THE RHEUMATIC DISEASES},
  keyword      = {RHEUMATOID-ARTHRITIS,DNA-SEQUENCING EQUIPMENT,DIFFERENTIAL-DIAGNOSIS,AGALACTOSYL IGG,GLYCOFORMS,GLYCOSYLATION,DISEASE,COMPLEMENT,N-LINKED OLIGOSACCHARIDES,PREGNANCY},
  language     = {eng},
  number       = {8},
  pages        = {1360--1365},
  title        = {Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis},
  url          = {http://dx.doi.org/10.1136/ard.2008.089292},
  volume       = {68},
  year         = {2009},
}

Chicago
Van Beneden, Katrien, Ken Coppieters, W Laroy, Filip De Keyser, Ilse Hoffman, Filip Van den Bosch, BERT VANDER CRUYSSEN, et al. 2009. “Reversible Changes in Serum Immunoglobulin Galactosylation During the Immune Response and Treatment of Inflammatory Autoimmune Arthritis.” Annals of the Rheumatic Diseases 68 (8): 1360–1365.
APA
Van Beneden, K., Coppieters, K., Laroy, W., De Keyser, F., Hoffman, I., Van den Bosch, F., VANDER CRUYSSEN, B., et al. (2009). Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis. ANNALS OF THE RHEUMATIC DISEASES, 68(8), 1360–1365.
Vancouver
1.
Van Beneden K, Coppieters K, Laroy W, De Keyser F, Hoffman I, Van den Bosch F, et al. Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis. ANNALS OF THE RHEUMATIC DISEASES. 2009;68(8):1360–5.
MLA
Van Beneden, Katrien, Ken Coppieters, W Laroy, et al. “Reversible Changes in Serum Immunoglobulin Galactosylation During the Immune Response and Treatment of Inflammatory Autoimmune Arthritis.” ANNALS OF THE RHEUMATIC DISEASES 68.8 (2009): 1360–1365. Print.