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Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery

Hui Li (UGent) , Kaat Fierens (UGent) , Zhiyue Zhang (UGent) , Nane Vanparijs (UGent) , Martijn Schuijs (UGent) , Katleen Van Steendam (UGent) , Natalia Feiner Gracia, Riet De Rycke (UGent) , Thomas De Beer (UGent) , Ans De Beuckelaer (UGent) , et al.
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Abstract
Nanomaterials hold potential of altering the interaction between therapeutic molecules and target cells or tissues. High aspect ratio nanomaterials in particular have been reported to possess unprecedented properties and are intensively investigated for their interaction with biological systems. Graphene oxide (GOx) is a water-soluble graphene derivative that combines high aspect ratio dimension with functional groups that can be exploited for bioconjugation. Here, we demonstrate that GOx nanosheets can spontaneously adsorb proteins by a combination of interactions. This property is then explored for intracellular protein vaccine delivery, in view of the potential of GOx nanosheets to destabilize lipid membranes such as those of intracellular vesicles. Using a series of in vitro experiments, we show that GOx nanosheet adsorbed proteins are efficiently internalized by dendritic cells (DCs: the most potent class of antigen presenting cells of the immune system) and promote antigen cross-presentation to CD8 T cells. The latter is a hallmark in the induction of potent cellular antigen-specific immune responses against intracellular pathogens and cancer.
Keywords
CANCER, NANOPARTICLES, T-LYMPHOCYTES, GRAPHITE OXIDE, MYCOBACTERIUM-TUBERCULOSIS, CROSS-PRESENTATION, dendritic cells, CARBON-BASED NANOMATERIALS, ANTIGEN, IMMUNITY, DENDRITIC CELLS, vaccines, nanosheets, protein, graphene oxide

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MLA
Li, Hui, Kaat Fierens, Zhiyue Zhang, et al. “Spontaneous Protein Adsorption on Graphene Oxide Nanosheets Allowing Efficient Intracellular Vaccine Protein Delivery.” ACS APPLIED MATERIALS & INTERFACES 8.2 (2016): 1147–1155. Print.
APA
Li, Hui, Fierens, K., Zhang, Z., Vanparijs, N., Schuijs, M., Van Steendam, K., Gracia, N. F., et al. (2016). Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery. ACS APPLIED MATERIALS & INTERFACES, 8(2), 1147–1155.
Chicago author-date
Li, Hui, Kaat Fierens, Zhiyue Zhang, Nane Vanparijs, Martijn Schuijs, Katleen Van Steendam, Natalia Feiner Gracia, et al. 2016. “Spontaneous Protein Adsorption on Graphene Oxide Nanosheets Allowing Efficient Intracellular Vaccine Protein Delivery.” Acs Applied Materials & Interfaces 8 (2): 1147–1155.
Chicago author-date (all authors)
Li, Hui, Kaat Fierens, Zhiyue Zhang, Nane Vanparijs, Martijn Schuijs, Katleen Van Steendam, Natalia Feiner Gracia, Riet De Rycke, Thomas De Beer, Ans De Beuckelaer, Stefaan De Koker, Dieter Deforce, Lorenzo Albertazzi, Johan Grooten, Bart Lambrecht, and Bruno De Geest. 2016. “Spontaneous Protein Adsorption on Graphene Oxide Nanosheets Allowing Efficient Intracellular Vaccine Protein Delivery.” Acs Applied Materials & Interfaces 8 (2): 1147–1155.
Vancouver
1.
Li H, Fierens K, Zhang Z, Vanparijs N, Schuijs M, Van Steendam K, et al. Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery. ACS APPLIED MATERIALS & INTERFACES. 2016;8(2):1147–55.
IEEE
[1]
H. Li et al., “Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery,” ACS APPLIED MATERIALS & INTERFACES, vol. 8, no. 2, pp. 1147–1155, 2016.
@article{7236459,
  abstract     = {Nanomaterials hold potential of altering the interaction between therapeutic molecules and target cells or tissues. High aspect ratio nanomaterials in particular have been reported to possess unprecedented properties and are intensively investigated for their interaction with biological systems. Graphene oxide (GOx) is a water-soluble graphene derivative that combines high aspect ratio dimension with functional groups that can be exploited for bioconjugation. Here, we demonstrate that GOx nanosheets can spontaneously adsorb proteins by a combination of interactions. This property is then explored for intracellular protein vaccine delivery, in view of the potential of GOx nanosheets to destabilize lipid membranes such as those of intracellular vesicles. Using a series of in vitro experiments, we show that GOx nanosheet adsorbed proteins are efficiently internalized by dendritic cells (DCs: the most potent class of antigen presenting cells of the immune system) and promote antigen cross-presentation to CD8 T cells. The latter is a hallmark in the induction of potent cellular antigen-specific immune responses against intracellular pathogens and cancer.},
  author       = {Li, Hui and Fierens, Kaat and Zhang, Zhiyue and Vanparijs, Nane and Schuijs, Martijn and Van Steendam, Katleen and Gracia, Natalia Feiner and De Rycke, Riet and De Beer, Thomas and De Beuckelaer, Ans and De Koker, Stefaan and Deforce, Dieter and Albertazzi, Lorenzo and Grooten, Johan and Lambrecht, Bart and De Geest, Bruno},
  issn         = {1944-8244},
  journal      = {ACS APPLIED MATERIALS & INTERFACES},
  keywords     = {CANCER,NANOPARTICLES,T-LYMPHOCYTES,GRAPHITE OXIDE,MYCOBACTERIUM-TUBERCULOSIS,CROSS-PRESENTATION,dendritic cells,CARBON-BASED NANOMATERIALS,ANTIGEN,IMMUNITY,DENDRITIC CELLS,vaccines,nanosheets,protein,graphene oxide},
  language     = {eng},
  number       = {2},
  pages        = {1147--1155},
  title        = {Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery},
  url          = {http://dx.doi.org/10.1021/acsami.5b08963},
  volume       = {8},
  year         = {2016},
}

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