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Clone-based functional genomics

Annick Bleys (UGent) , Mansour Karimi (UGent) and Pierre Hilson (UGent)
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Abstract
Annotated genomes have provided a wealth of information about gene structure and gene catalogs in a wide range of species. Taking advantage of these developments, novel techniques have been implemented to investigate systematically diverse aspects of gene and protein functions underpinning biology processes. Here, we review functional genomics applications that require the mass production of cloned sequence repertoires, including ORFeomes and silencing tag collections. We discuss the techniques employed in large-scale cloning projects and we provide an up-to-date overview of the clone resources available for model plant species and of the current applications that may be scaled up for systematic plant gene studies.
Keywords
clone collections, Functional genomics, ORFeome, hairpin RNA, artificial microRNA, recombinational cloning

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Citation

Please use this url to cite or link to this publication:

Chicago
Bleys, Annick, Mansour Karimi, and Pierre Hilson. 2009. “Clone-based Functional Genomics.” In Plant Systems Biology, ed. Dmitry Belostotsky, 553:141–177. Totowa, NJ, USA: Humana Press.
APA
Bleys, A., Karimi, M., & Hilson, P. (2009). Clone-based functional genomics. In D. Belostotsky (Ed.), Plant systems biology (Vol. 553, pp. 141–177). Totowa, NJ, USA: Humana Press.
Vancouver
1.
Bleys A, Karimi M, Hilson P. Clone-based functional genomics. In: Belostotsky D, editor. Plant systems biology. Totowa, NJ, USA: Humana Press; 2009. p. 141–77.
MLA
Bleys, Annick, Mansour Karimi, and Pierre Hilson. “Clone-based Functional Genomics.” Plant Systems Biology. Ed. Dmitry Belostotsky. Vol. 553. Totowa, NJ, USA: Humana Press, 2009. 141–177. Print.
@incollection{723111,
  abstract     = {Annotated genomes have provided a wealth of information about gene structure and gene catalogs in a wide range of species. Taking advantage of these developments, novel techniques have been implemented to investigate systematically diverse aspects of gene and protein functions underpinning biology processes. Here, we review functional genomics applications that require the mass production of cloned sequence repertoires, including ORFeomes and silencing tag collections. We discuss the techniques employed in large-scale cloning projects and we provide an up-to-date overview of the clone resources available for model plant species and of the current applications that may be scaled up for systematic plant gene studies.},
  author       = {Bleys, Annick and Karimi, Mansour and Hilson, Pierre},
  booktitle    = {Plant systems biology},
  editor       = {Belostotsky, Dmitry},
  isbn         = {9781603275620},
  language     = {eng},
  pages        = {141--177},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Clone-based functional genomics},
  url          = {http://dx.doi.org/10.1007/978-1-60327-563-7\_8},
  volume       = {553},
  year         = {2009},
}

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