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Abstract
Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)(5) primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) (=CCM 8529(T)=LMG 28327(T)).
Keywords
GENE, SPECIES IDENTIFICATION, HYBRIDIZATION, JETTENSIS, SPECIMENS

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MLA
Švec, Pavel et al. “Staphylococcus Petrasii Subsp. Pragensis Subsp. Nov., Occurring in Human Clinical Material.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65.7 (2015): 2071–2077. Print.
APA
Švec, P., De Bel, A., Sedláček, I., Petráš, P., Gelbíčová, T., Černohlávková, J., Mašlaňová, I., et al. (2015). Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 65(7), 2071–2077.
Chicago author-date
Švec, Pavel, Annelies De Bel, Ivo Sedláček, Petr Petráš, Tereza Gelbíčová, Jitka Černohlávková, Ivana Mašlaňová, et al. 2015. “Staphylococcus Petrasii Subsp. Pragensis Subsp. Nov., Occurring in Human Clinical Material.” International Journal of Systematic and Evolutionary Microbiology 65 (7): 2071–2077.
Chicago author-date (all authors)
Švec, Pavel, Annelies De Bel, Ivo Sedláček, Petr Petráš, Tereza Gelbíčová, Jitka Černohlávková, Ivana Mašlaňová, Margo Cnockaert, Ivana Varbanovová, Fedoua Echahidi, Peter Vandamme, and Roman Pantůček. 2015. “Staphylococcus Petrasii Subsp. Pragensis Subsp. Nov., Occurring in Human Clinical Material.” International Journal of Systematic and Evolutionary Microbiology 65 (7): 2071–2077.
Vancouver
1.
Švec P, De Bel A, Sedláček I, Petráš P, Gelbíčová T, Černohlávková J, et al. Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2015;65(7):2071–7.
IEEE
[1]
P. Švec et al., “Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 65, no. 7, pp. 2071–2077, 2015.
@article{7225558,
  abstract     = {Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)(5) primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) (=CCM 8529(T)=LMG 28327(T)).},
  author       = {Švec, Pavel and De Bel, Annelies and Sedláček, Ivo and Petráš, Petr and Gelbíčová, Tereza and Černohlávková, Jitka and Mašlaňová, Ivana and Cnockaert, Margo and Varbanovová, Ivana and Echahidi, Fedoua and Vandamme, Peter and Pantůček, Roman},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {GENE,SPECIES IDENTIFICATION,HYBRIDIZATION,JETTENSIS,SPECIMENS},
  language     = {eng},
  number       = {7},
  pages        = {2071--2077},
  title        = {Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material},
  url          = {http://dx.doi.org/10.1099/ijs.0.000220},
  volume       = {65},
  year         = {2015},
}

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