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Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk

Zaina Kadri (UGent) , Freek Spitaels (UGent) , Margo Cnockaert (UGent) , Jessy Praet (UGent) , Omar El Farricha, Jean Swings (UGent) and Peter Vandamme (UGent)
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Abstract
Four lactic acid bacteria isolates obtained from fresh dromedary camel milk produced in Dakhla, a city in southern Morocco, were characterised in order to determine their taxonomic position. The four isolates had highly similar MALDI-TOF MS and RAPD fingerprints and identical 16S rRNA gene sequences. Comparative sequence analysis revealed that the 16S rRNA gene sequence of the four isolates was most similar to that of Enterococcus sulfureus ATCC 49903(T) and Enterococcus italicus DSM 15952(T) (99.33 and 98.59 % similarity, respectively). However, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes revealed that the taxon represented by strain LMG 28766(T) was well separated from E. sulfureus LMG 13084(T) and E. italicus LMG 22039(T), which was further confirmed by DNA-DNA hybridization values that were clearly below the species demarcation threshold. The novel taxon was easily differentiated from its nearest neighbour species through sequence analysis of protein encoding genes, MALDI-TOF mass spectrometry and multiple biochemical tests, but had a similar percentage G+C content of about 39 %. We therefore propose to formally classify these isolates as Enterococcus bulliens sp. nov., with LMG 28766(T) (=CCMM B1177(T)) as the type strain.
Keywords
Lactic acid bacteria, Enterococcus bulliens sp nov., Camel milk microbiota, Fresh dromedary milk, Taxonomy, DEOXYRIBONUCLEIC-ACID, DAIRY-PRODUCTS, STREPTOCOCCUS, STRAINS, FAECIUM, GENES, WATER

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Chicago
Kadri, Zaina, Freek Spitaels, Margo Cnockaert, Jessy Praet, Omar El Farricha, Jean Swings, and Peter Vandamme. 2015. “Enterococcus Bulliens Sp. Nov., a Novel Lactic Acid Bacterium Isolated from Camel Milk.” Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 108 (5): 1257–1265.
APA
Kadri, Z., Spitaels, F., Cnockaert, M., Praet, J., El Farricha, O., Swings, J., & Vandamme, P. (2015). Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 108(5), 1257–1265.
Vancouver
1.
Kadri Z, Spitaels F, Cnockaert M, Praet J, El Farricha O, Swings J, et al. Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. 2015;108(5):1257–65.
MLA
Kadri, Zaina, Freek Spitaels, Margo Cnockaert, et al. “Enterococcus Bulliens Sp. Nov., a Novel Lactic Acid Bacterium Isolated from Camel Milk.” ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 108.5 (2015): 1257–1265. Print.
@article{7225541,
  abstract     = {Four lactic acid bacteria isolates obtained from fresh dromedary camel milk produced in Dakhla, a city in southern Morocco, were characterised in order to determine their taxonomic position. The four isolates had highly similar MALDI-TOF MS and RAPD fingerprints and identical 16S rRNA gene sequences. Comparative sequence analysis revealed that the 16S rRNA gene sequence of the four isolates was most similar to that of Enterococcus sulfureus ATCC 49903(T) and Enterococcus italicus DSM 15952(T) (99.33 and 98.59 \% similarity, respectively). However, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes revealed that the taxon represented by strain LMG 28766(T) was well separated from E. sulfureus LMG 13084(T) and E. italicus LMG 22039(T), which was further confirmed by DNA-DNA hybridization values that were clearly below the species demarcation threshold. The novel taxon was easily differentiated from its nearest neighbour species through sequence analysis of protein encoding genes, MALDI-TOF mass spectrometry and multiple biochemical tests, but had a similar percentage G+C content of about 39 \%. We therefore propose to formally classify these isolates as Enterococcus bulliens sp. nov., with LMG 28766(T) (=CCMM B1177(T)) as the type strain.},
  author       = {Kadri, Zaina and Spitaels, Freek and Cnockaert, Margo and Praet, Jessy and El Farricha, Omar and Swings, Jean and Vandamme, Peter},
  issn         = {0003-6072},
  journal      = {ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {1257--1265},
  title        = {Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk},
  url          = {http://dx.doi.org/10.1007/s10482-015-0579-z},
  volume       = {108},
  year         = {2015},
}

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