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Bifidobacterium aquikefiri sp nov., isolated from water kefir

David Laureys (UGent) , Margo Cnockaert (UGent) , Luc De Vuyst and Peter Vandamme (UGent)
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Abstract
A novel Bifidobacterium, strain LMG 28769(T), was isolated from a household water kefir fermentation process. Cells were Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, oxidase-negative and facultatively anaerobic short rods. Analysis of its 16S rRNA gene sequence revealed Bifidobacterium crudilactis and Bifidobacterium psychraerophilum (97.4 and 97.1 % similarity towards the respective type strain sequences) as nearest phylogenetic neighbours. Its assignment to the genus Bifidobacterium was confirmed by the presence of fructose 6-phosphate phosphoketolase activity. Analysis of the hsp60 gene sequence revealed very low similarity with nucleotide sequences in the NCBI nucleotide database. The genotypic and phenotypic analyses allowed the differentiation of strain LMG 28769(T) from all recognized Bifidobacterium species. Strain LMG 28769(T) (=CCUG 67145(T) =R 54638(T)) therefore represents a novel species, for which the name Bifidobacterium aquikefiri sp. nov. is proposed.
Keywords
LACTOBACILLUS, DNA, SEQUENCES, DATABASE, MILK

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Chicago
Laureys, David, Margo Cnockaert, Luc De Vuyst, and Peter Vandamme. 2016. “Bifidobacterium Aquikefiri Sp Nov., Isolated from Water Kefir.” International Journal of Systematic and Evolutionary Microbiology 66 (3): 1281–1286.
APA
Laureys, D., Cnockaert, M., De Vuyst, L., & Vandamme, P. (2016). Bifidobacterium aquikefiri sp nov., isolated from water kefir. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 66(3), 1281–1286.
Vancouver
1.
Laureys D, Cnockaert M, De Vuyst L, Vandamme P. Bifidobacterium aquikefiri sp nov., isolated from water kefir. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2016;66(3):1281–6.
MLA
Laureys, David, Margo Cnockaert, Luc De Vuyst, et al. “Bifidobacterium Aquikefiri Sp Nov., Isolated from Water Kefir.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66.3 (2016): 1281–1286. Print.
@article{7225510,
  abstract     = {A novel Bifidobacterium, strain LMG 28769(T), was isolated from a household water kefir fermentation process. Cells were Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, oxidase-negative and facultatively anaerobic short rods. Analysis of its 16S rRNA gene sequence revealed Bifidobacterium crudilactis and Bifidobacterium psychraerophilum (97.4 and 97.1 \% similarity towards the respective type strain sequences) as nearest phylogenetic neighbours. Its assignment to the genus Bifidobacterium was confirmed by the presence of fructose 6-phosphate phosphoketolase activity. Analysis of the hsp60 gene sequence revealed very low similarity with nucleotide sequences in the NCBI nucleotide database. The genotypic and phenotypic analyses allowed the differentiation of strain LMG 28769(T) from all recognized Bifidobacterium species. Strain LMG 28769(T) (=CCUG 67145(T) =R 54638(T)) therefore represents a novel species, for which the name Bifidobacterium aquikefiri sp. nov. is proposed.},
  author       = {Laureys, David and Cnockaert, Margo and De Vuyst, Luc and Vandamme, Peter},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {LACTOBACILLUS,DNA,SEQUENCES,DATABASE,MILK},
  language     = {eng},
  number       = {3},
  pages        = {1281--1286},
  title        = {Bifidobacterium aquikefiri sp nov., isolated from water kefir},
  url          = {http://dx.doi.org/10.1099/ijsem.0.000877},
  volume       = {66},
  year         = {2016},
}

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