Advanced search
1 file | 13.86 MB

The Wnt co-receptor Lrp5 is required for cranial neural crest cell migration in zebrafish

(2015) PLOS ONE. 10(6).
Author
Organization
Abstract
During vertebrate neurulation, cranial neural crest cells (CNCCs) undergo epithelial to mesenchymal transition (EMT), delaminate from the neural plate border, and migrate as separate streams into different cranial regions. There, they differentiate into distinct parts of the craniofacial skeleton. Canonical Wnt signaling has been shown to be essential for this process at different levels but the involved receptors remained unclear. Here we show that the frizzled co-receptor low-density-lipoprotein (LDL) receptor-related protein 5 (Lrp5) plays a crucial role in CNCC migration and morphogenesis of the cranial skeleton. Early during induction and migration of CNCCs, lrp5 is expressed ubiquitously but later gets restricted to CNCC derivatives in the ventral head region besides different regions in the CNS. A knock-down of lrp5 does not interfere with induction of CNCCs but leads to reduced proliferation of premigratory CNCCs. In addition, cell migration is disrupted as CNCCs are found in clusters at ectopic positions in the dorsomedial neuroepithelium after lrp5 knock-down and transient CRISPR/Cas9 gene editing. These migratory defects consequently result in malformations of the craniofacial skeleton. To date, Lrp5 has mainly been associated with bone homeostasis in mammals. Here we show that in zebrafish, lrp5 also controls cell migration during early morphogenetic processes and contributes to shaping the craniofacial skeleton.
Keywords
BONE-DENSITY, EMBRYONIC-DEVELOPMENT, RECEPTOR-RELATED PROTEIN-5, PHARYNGEAL DENTITION, G1/S TRANSITION, REITERATED WNT, BETA-CATENIN, EXPRESSION, SPECIFICATION, SCLEROSTIN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 13.86 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Willems, Berndt, Shijie Tao, Tingsheng Yu, Ann Huysseune, Paul Witten, and Christoph Winkler. 2015. “The Wnt Co-receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish.” Plos One 10 (6).
APA
Willems, Berndt, Tao, S., Yu, T., Huysseune, A., Witten, P., & Winkler, C. (2015). The Wnt co-receptor Lrp5 is required for cranial neural crest cell migration in zebrafish. PLOS ONE, 10(6).
Vancouver
1.
Willems B, Tao S, Yu T, Huysseune A, Witten P, Winkler C. The Wnt co-receptor Lrp5 is required for cranial neural crest cell migration in zebrafish. PLOS ONE. 2015;10(6).
MLA
Willems, Berndt, Shijie Tao, Tingsheng Yu, et al. “The Wnt Co-receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish.” PLOS ONE 10.6 (2015): n. pag. Print.
@article{7223725,
  abstract     = {During vertebrate neurulation, cranial neural crest cells (CNCCs) undergo epithelial to mesenchymal transition (EMT), delaminate from the neural plate border, and migrate as separate streams into different cranial regions. There, they differentiate into distinct parts of the craniofacial skeleton. Canonical Wnt signaling has been shown to be essential for this process at different levels but the involved receptors remained unclear. Here we show that the frizzled co-receptor low-density-lipoprotein (LDL) receptor-related protein 5 (Lrp5) plays a crucial role in CNCC migration and morphogenesis of the cranial skeleton. Early during induction and migration of CNCCs, lrp5 is expressed ubiquitously but later gets restricted to CNCC derivatives in the ventral head region besides different regions in the CNS. A knock-down of lrp5 does not interfere with induction of CNCCs but leads to reduced proliferation of premigratory CNCCs. In addition, cell migration is disrupted as CNCCs are found in clusters at ectopic positions in the dorsomedial neuroepithelium after lrp5 knock-down and transient CRISPR/Cas9 gene editing. These migratory defects consequently result in malformations of the craniofacial skeleton. To date, Lrp5 has mainly been associated with bone homeostasis in mammals. Here we show that in zebrafish, lrp5 also controls cell migration during early morphogenetic processes and contributes to shaping the craniofacial skeleton.},
  articleno    = {e0131768},
  author       = {Willems, Berndt and Tao, Shijie and Yu, Tingsheng and Huysseune, Ann and Witten, Paul and Winkler, Christoph},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {BONE-DENSITY,EMBRYONIC-DEVELOPMENT,RECEPTOR-RELATED PROTEIN-5,PHARYNGEAL DENTITION,G1/S TRANSITION,REITERATED WNT,BETA-CATENIN,EXPRESSION,SPECIFICATION,SCLEROSTIN},
  language     = {eng},
  number       = {6},
  pages        = {21},
  title        = {The Wnt co-receptor Lrp5 is required for cranial neural crest cell migration in zebrafish},
  url          = {http://dx.doi.org/10.1371/journal.pone.0131768},
  volume       = {10},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: