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Biocidal properties of a glycosylated surface: sophorolipids on Au(111)

(2015) ACS APPLIED MATERIALS & INTERFACES. 7(32). p.18086-18095
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Abstract
Classical antibacterial surfaces usually involve antiadhesive and/or biocidal strategies. Glycosylated surfaces are usually used to prevent biofilm formation via antiadhesive mechanisms. We report here the first example of a glycosylated surface with biocidal properties created by the covalent grafting of sophorolipids (a sophorose unit linked by a glycosidic bond to an oleic acid) through a self-assembled monolayer (SAM) of short aminothiols on gold (111) surfaces. The biocidal effect of such surfaces on Gram+ bacteria was assessed by a wide combination of techniques including microscopy observations, fluorescent staining, and bacterial growth tests. About 50% of the bacteria are killed via alteration of the cell envelope. In addition, the roles of the sophorose unit and aliphatic chain configuration are highlighted by the lack of activity of substrates modified, respectively, with sophorose-free oleic acid and sophorolipid-derivative having a saturated aliphatic chain. This system demonstrates thus the direct implication of a carbohydrate in the destabilization and disruption of the bacterial cell envelope.
Keywords
glycolipid, sophorolipid, surface functionalization, self-assembled monolayer, antibacterial coating, killing-by-contact, biocidal mechanism, ESCHERICHIA-COLI, ANTIMICROBIAL PEPTIDES, NATURAL-ENVIRONMENT, LIQUID-CRYSTALS, BACTERIA, BIOSURFACTANTS, MEMBRANE, BIOFILMS, TRANSGLYCOSYLATION, POLYMERIZATION

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MLA
Valotteau, Claire et al. “Biocidal Properties of a Glycosylated Surface: Sophorolipids on Au(111).” ACS APPLIED MATERIALS & INTERFACES 7.32 (2015): 18086–18095. Print.
APA
Valotteau, C., Calers, C., Casle, S., Berton, J., Stevens, C., Babonneau, F., Padrier, C.-M., et al. (2015). Biocidal properties of a glycosylated surface: sophorolipids on Au(111). ACS APPLIED MATERIALS & INTERFACES, 7(32), 18086–18095.
Chicago author-date
Valotteau, Claire, Christophe Calers, Sandra Casle, Jan Berton, Christian Stevens, Florence Babonneau, Claire-Marie Padrier, Vincent Humblot, and Niki Baccile. 2015. “Biocidal Properties of a Glycosylated Surface: Sophorolipids on Au(111).” Acs Applied Materials & Interfaces 7 (32): 18086–18095.
Chicago author-date (all authors)
Valotteau, Claire, Christophe Calers, Sandra Casle, Jan Berton, Christian Stevens, Florence Babonneau, Claire-Marie Padrier, Vincent Humblot, and Niki Baccile. 2015. “Biocidal Properties of a Glycosylated Surface: Sophorolipids on Au(111).” Acs Applied Materials & Interfaces 7 (32): 18086–18095.
Vancouver
1.
Valotteau C, Calers C, Casle S, Berton J, Stevens C, Babonneau F, et al. Biocidal properties of a glycosylated surface: sophorolipids on Au(111). ACS APPLIED MATERIALS & INTERFACES. 2015;7(32):18086–95.
IEEE
[1]
C. Valotteau et al., “Biocidal properties of a glycosylated surface: sophorolipids on Au(111),” ACS APPLIED MATERIALS & INTERFACES, vol. 7, no. 32, pp. 18086–18095, 2015.
@article{7220761,
  abstract     = {Classical antibacterial surfaces usually involve antiadhesive and/or biocidal strategies. Glycosylated surfaces are usually used to prevent biofilm formation via antiadhesive mechanisms. We report here the first example of a glycosylated surface with biocidal properties created by the covalent grafting of sophorolipids (a sophorose unit linked by a glycosidic bond to an oleic acid) through a self-assembled monolayer (SAM) of short aminothiols on gold (111) surfaces. The biocidal effect of such surfaces on Gram+ bacteria was assessed by a wide combination of techniques including microscopy observations, fluorescent staining, and bacterial growth tests. About 50% of the bacteria are killed via alteration of the cell envelope. In addition, the roles of the sophorose unit and aliphatic chain configuration are highlighted by the lack of activity of substrates modified, respectively, with sophorose-free oleic acid and sophorolipid-derivative having a saturated aliphatic chain. This system demonstrates thus the direct implication of a carbohydrate in the destabilization and disruption of the bacterial cell envelope.},
  author       = {Valotteau, Claire and Calers, Christophe and Casle, Sandra and Berton, Jan and Stevens, Christian and Babonneau, Florence and Padrier, Claire-Marie and Humblot, Vincent and Baccile, Niki},
  issn         = {1944-8244},
  journal      = {ACS APPLIED MATERIALS & INTERFACES},
  keywords     = {glycolipid,sophorolipid,surface functionalization,self-assembled monolayer,antibacterial coating,killing-by-contact,biocidal mechanism,ESCHERICHIA-COLI,ANTIMICROBIAL PEPTIDES,NATURAL-ENVIRONMENT,LIQUID-CRYSTALS,BACTERIA,BIOSURFACTANTS,MEMBRANE,BIOFILMS,TRANSGLYCOSYLATION,POLYMERIZATION},
  language     = {eng},
  number       = {32},
  pages        = {18086--18095},
  title        = {Biocidal properties of a glycosylated surface: sophorolipids on Au(111)},
  url          = {http://dx.doi.org/10.1021/acsami.5b05090},
  volume       = {7},
  year         = {2015},
}

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