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Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

(2009) Nature. 459(7250). p.1136-1140
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Abstract
The plant signalling molecule auxin provides positional information in a variety of developmental processes by means of its differential distribution (gradients) within plant tissues(1). Thus, cellular auxin levels often determine the developmental output of auxin signalling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell(2,3). In particular, PIN auxin-efflux-carrier-mediated, directional transport between cells is crucial for generating auxin gradients(2,4,5). Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development. PIN5 does not have a direct role in cell-to-cell transport but regulates intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmic reticulum ( ER), presumably mediating auxin flow from the cytosol to the lumen of the ER. The ER localization of other PIN5-like transporters ( including the moss PIN) indicates that the diversification of PIN protein functions in mediating auxin homeostasis at the ER, and cell-to-cell auxin transport at the plasma membrane, represent an ancient event during the evolution of land plants.
Keywords
EFFLUX, GRADIENTS, ARABIDOPSIS, TWISTED DWARF1, PLASMA-MEMBRANE, PLANT TRANSFORMATION, BOX PROTEIN TIR1, RECEPTOR, LAND, FLOW

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Chicago
Mravec, Jozef, Petr Skupa, Aurélien Bailly, Klára Hoyerová, Pavel Krecek, Agnieszka Bielach, Jan Petrásek, et al. 2009. “Subcellular Homeostasis of Phytohormone Auxin Is Mediated by the ER-localized PIN5 Transporter.” Nature 459 (7250): 1136–1140.
APA
Mravec, J., Skupa, P., Bailly, A., Hoyerová, K., Krecek, P., Bielach, A., Petrásek, J., et al. (2009). Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter. Nature, 459(7250), 1136–1140.
Vancouver
1.
Mravec J, Skupa P, Bailly A, Hoyerová K, Krecek P, Bielach A, et al. Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter. Nature. 2009;459(7250):1136–40.
MLA
Mravec, Jozef, Petr Skupa, Aurélien Bailly, et al. “Subcellular Homeostasis of Phytohormone Auxin Is Mediated by the ER-localized PIN5 Transporter.” Nature 459.7250 (2009): 1136–1140. Print.
@article{721933,
  abstract     = {The plant signalling molecule auxin provides positional information in a variety of developmental processes by means of its differential distribution (gradients) within plant tissues(1). Thus, cellular auxin levels often determine the developmental output of auxin signalling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell(2,3). In particular, PIN auxin-efflux-carrier-mediated, directional transport between cells is crucial for generating auxin gradients(2,4,5). Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development. PIN5 does not have a direct role in cell-to-cell transport but regulates intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmic reticulum ( ER), presumably mediating auxin flow from the cytosol to the lumen of the ER. The ER localization of other PIN5-like transporters ( including the moss PIN) indicates that the diversification of PIN protein functions in mediating auxin homeostasis at the ER, and cell-to-cell auxin transport at the plasma membrane, represent an ancient event during the evolution of land plants.},
  author       = {Mravec, Jozef and Skupa, Petr and Bailly, Aur{\'e}lien and Hoyerov{\'a}, Kl{\'a}ra and Krecek, Pavel and Bielach, Agnieszka and Petr{\'a}sek, Jan and Zhang, Jing and Gaykova, Vassilena and Stierhof, York-Dieter and Dobrev, Petre and Schwarzerov{\'a}, Katerina and Rolc{\'i}k, Jakub and Seifertov{\'a}, Daniela and Luschnig, Christian and Benkova, Eva and Zazimalov{\'a}, Eva and Geisler, Markus and Friml, Jiri},
  issn         = {0028-0836},
  journal      = {Nature},
  keyword      = {EFFLUX,GRADIENTS,ARABIDOPSIS,TWISTED DWARF1,PLASMA-MEMBRANE,PLANT TRANSFORMATION,BOX PROTEIN TIR1,RECEPTOR,LAND,FLOW},
  language     = {eng},
  number       = {7250},
  pages        = {1136--1140},
  title        = {Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter},
  url          = {http://dx.doi.org/10.1038/nature08066},
  volume       = {459},
  year         = {2009},
}

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