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APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root

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Abstract
Plant organs originate from meristems where stem cells are maintained to produce continuously daughter cells that are the source of different cell types. The cell cycle switch gene CCS52A, a substrate specific activator of the anaphase promoting complex/cyclosome (APC/C), controls the mitotic arrest and the transition of mitotic cycles to endoreduplication (ER) cycles as part of cell differentiation. Arabidopsis, unlike other organisms, contains 2 CCS52A isoforms. Here, we show that both of them are active and regulate meristem maintenance in the root tip, although through different mechanisms. The CCS52A1 activity in the elongation zone of the root stimulates ER and mitotic exit, and contributes to the border delineation between dividing and expanding cells. In contrast, CCS52A2 acts directly in the distal region of the root meristem to control identity of the quiescent center (QC) cells and stem cell maintenance. Cell proliferation assays in roots suggest that this control involves CCS52A2 mediated repression of mitotic activity in the QC cells. The data indicate that the CCS52A genes favor a low mitotic state in different cell types of the root tip that is required for meristem maintenance, and reveal a previously undescribed mechanism for APC/C mediated control in plant development.
Keywords
LOCALIZATION, ORGANIZATION, ACTIVATOR, ENDOREDUPLICATION, DROSOPHILA, CDC27 HOMOLOG, DIFFERENTIATION, RETINOBLASTOMA PROTEIN, PLANT-CELL CYCLE, ANAPHASE-PROMOTING COMPLEX, stem cells, cell differentiation, endoreduplication, CDH1, quiescent center

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Chicago
Vanstraelen, Marleen, Mikhail Baloban, Olivier Da Ines, Antonietta Cultrone, Tim Lammens, Véronique Boudolf, Spencer Brown, Lieven De Veylder, Peter Mergaert, and Eva Kondorosi. 2009. “APC/C-CCS52A Complexes Control Meristem Maintenance in the Arabidopsis Root.” Proceedings of the National Academy of Sciences of the United States of America 106 (28): 11806–11811.
APA
Vanstraelen, M., Baloban, M., Da Ines, O., Cultrone, A., Lammens, T., Boudolf, V., Brown, S., et al. (2009). APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(28), 11806–11811.
Vancouver
1.
Vanstraelen M, Baloban M, Da Ines O, Cultrone A, Lammens T, Boudolf V, et al. APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2009;106(28):11806–11.
MLA
Vanstraelen, Marleen, Mikhail Baloban, Olivier Da Ines, et al. “APC/C-CCS52A Complexes Control Meristem Maintenance in the Arabidopsis Root.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106.28 (2009): 11806–11811. Print.
@article{721922,
  abstract     = {Plant organs originate from meristems where stem cells are maintained to produce continuously daughter cells that are the source of different cell types. The cell cycle switch gene CCS52A, a substrate specific activator of the anaphase promoting complex/cyclosome (APC/C), controls the mitotic arrest and the transition of mitotic cycles to endoreduplication (ER) cycles as part of cell differentiation. Arabidopsis, unlike other organisms, contains 2 CCS52A isoforms. Here, we show that both of them are active and regulate meristem maintenance in the root tip, although through different mechanisms. The CCS52A1 activity in the elongation zone of the root stimulates ER and mitotic exit, and contributes to the border delineation between dividing and expanding cells. In contrast, CCS52A2 acts directly in the distal region of the root meristem to control identity of the quiescent center (QC) cells and stem cell maintenance. Cell proliferation assays in roots suggest that this control involves CCS52A2 mediated repression of mitotic activity in the QC cells. The data indicate that the CCS52A genes favor a low mitotic state in different cell types of the root tip that is required for meristem maintenance, and reveal a previously undescribed mechanism for APC/C mediated control in plant development.},
  author       = {Vanstraelen, Marleen and Baloban, Mikhail and Da Ines, Olivier and Cultrone, Antonietta and Lammens, Tim and Boudolf, V{\'e}ronique and Brown, Spencer and De Veylder, Lieven and Mergaert, Peter and Kondorosi, Eva},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {LOCALIZATION,ORGANIZATION,ACTIVATOR,ENDOREDUPLICATION,DROSOPHILA,CDC27 HOMOLOG,DIFFERENTIATION,RETINOBLASTOMA PROTEIN,PLANT-CELL CYCLE,ANAPHASE-PROMOTING COMPLEX,stem cells,cell differentiation,endoreduplication,CDH1,quiescent center},
  language     = {eng},
  number       = {28},
  pages        = {11806--11811},
  title        = {APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root},
  url          = {http://dx.doi.org/10.1073/pnas.0901193106},
  volume       = {106},
  year         = {2009},
}

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