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Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana

Matthew Ramon, Ive De Smet UGent, Lies Vandesteene, Mirande Naudts UGent, Barbara Leyman UGent, Patrick Van Dijck, Filip Rolland, Tom Beeckman UGent and Johan M. Thevelein (2009) PLANT CELL AND ENVIRONMENT. 32(8). p.1015-1032
abstract
Trehalose metabolism has profound effects on plant growth and metabolism, but the mechanisms involved are unclear. In Arabidopsis, 21 putative trehalose biosynthesis genes are classified in three subfamilies based on their similarity with yeast TPS1 (encoding a trehalose-6-phosphate synthase, TPS) or TPS2 (encoding a trehalose-6-phosphate phosphatase, TPP). Although TPS1 (Class I) and TPPA and TPPB (Class III) proteins have established TPS and TPP activity, respectively, the function of the Class II proteins (AtTPS5-AtTPS11) remains elusive. A complete set of promoter-beta-glucurinidase/green fluorescent protein reporters demonstrates their remarkably differential tissue-specific expression and responsiveness to carbon availability and hormones. Heterologous expression in yeast furthermore suggests that none of the encoded enzymes displays significant TPS or TPP activity, consistent with a regulatory rather than metabolic function for this remarkable class of proteins.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Class II TPS genes, sugar-and hormone responses, trehalose metabolism, Arabidopsis thaliana, expression regulation, metabolic activity
journal title
PLANT CELL AND ENVIRONMENT
Plant Cell Environ.
volume
32
issue
8
pages
1015 - 1032
Web of Science type
Article
Web of Science id
000267884800007
JCR category
PLANT SCIENCES
JCR impact factor
5.081 (2009)
JCR rank
9/172 (2009)
JCR quartile
1 (2009)
ISSN
0140-7791
DOI
10.1111/j.1365-3040.2009.01985.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
721893
handle
http://hdl.handle.net/1854/LU-721893
date created
2009-08-04 20:02:15
date last changed
2016-12-19 15:40:12
@article{721893,
  abstract     = {Trehalose metabolism has profound effects on plant growth and metabolism, but the mechanisms involved are unclear. In Arabidopsis, 21 putative trehalose biosynthesis genes are classified in three subfamilies based on their similarity with yeast TPS1 (encoding a trehalose-6-phosphate synthase, TPS) or TPS2 (encoding a trehalose-6-phosphate phosphatase, TPP). Although TPS1 (Class I) and TPPA and TPPB (Class III) proteins have established TPS and TPP activity, respectively, the function of the Class II proteins (AtTPS5-AtTPS11) remains elusive. A complete set of promoter-beta-glucurinidase/green fluorescent protein reporters demonstrates their remarkably differential tissue-specific expression and responsiveness to carbon availability and hormones. Heterologous expression in yeast furthermore suggests that none of the encoded enzymes displays significant TPS or TPP activity, consistent with a regulatory rather than metabolic function for this remarkable class of proteins.},
  author       = {Ramon, Matthew and De Smet, Ive and Vandesteene, Lies and Naudts, Mirande and Leyman, Barbara and Van Dijck, Patrick and Rolland, Filip and Beeckman, Tom and Thevelein, Johan M.},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  keyword      = {Class II TPS genes,sugar-and hormone responses,trehalose metabolism,Arabidopsis thaliana,expression regulation,metabolic activity},
  language     = {eng},
  number       = {8},
  pages        = {1015--1032},
  title        = {Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1111/j.1365-3040.2009.01985.x},
  volume       = {32},
  year         = {2009},
}

Chicago
Ramon, Matthew, Ive De Smet, Lies Vandesteene, Mirande Naudts, Barbara Leyman, Patrick Van Dijck, Filip Rolland, Tom Beeckman, and Johan M. Thevelein. 2009. “Extensive Expression Regulation and Lack of Heterologous Enzymatic Activity of the Class II Trehalose Metabolism Proteins from Arabidopsis Thaliana.” Plant Cell and Environment 32 (8): 1015–1032.
APA
Ramon, M., De Smet, I., Vandesteene, L., Naudts, M., Leyman, B., Van Dijck, P., Rolland, F., et al. (2009). Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana. PLANT CELL AND ENVIRONMENT, 32(8), 1015–1032.
Vancouver
1.
Ramon M, De Smet I, Vandesteene L, Naudts M, Leyman B, Van Dijck P, et al. Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana. PLANT CELL AND ENVIRONMENT. 2009;32(8):1015–32.
MLA
Ramon, Matthew, Ive De Smet, Lies Vandesteene, et al. “Extensive Expression Regulation and Lack of Heterologous Enzymatic Activity of the Class II Trehalose Metabolism Proteins from Arabidopsis Thaliana.” PLANT CELL AND ENVIRONMENT 32.8 (2009): 1015–1032. Print.