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Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens

(2016) JOURNAL OF DAIRY SCIENCE. 99(4). p.2940-2944
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Abstract
Despite the increasing knowledge of prevention and control strategies, bovine mastitis remains one of the most challenging diseases in the dairy industry. This study investigated the antimicrobial activity of 13 species of lactic acid bacteria (LAB), previously isolated from the honey crop of the honeybee, on several mastitis pathogens. The viable LAB were first reintroduced into a sterilized heather honey matrix. More than 20 different bovine mastitis isolates were tested against the mixture of the 13 LAB species in the honey medium using a dual-culture overlay assay. The mastitis isolates were identified through bacteriological culturing, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Additionally, the mastitis isolates were subjected to antimicrobial susceptibility testing through disk diffusion. Growth of all tested mastitis pathogens, including the ones displaying antimicrobial resistance to one or more antimicrobial compounds, were inhibited to some extent by the honey and LAB combination. The antibacterial effect of these LAB opens up new perspectives on alternative treatment and prevention of bovine mastitis.
Keywords
APIS-MELLIFERA, ANTIBACTERIAL ACTIVITY, honey, lactic acid bacteria, bovine mastitis, BOVINE MASTITIS, DAIRY-COWS, IDENTIFICATION, ANIMALS, ORIGIN, IMPACT, FLORA

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MLA
Piccart, Kristine et al. “Short Communication: Lactic Acid Bacteria from the Honeybee Inhibit the in Vitro Growth of Mastitis Pathogens.” JOURNAL OF DAIRY SCIENCE 99.4 (2016): 2940–2944. Print.
APA
Piccart, K., Vasquez, A., Piepers, S., De Vliegher, S., & Olofsson, T. (2016). Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens. JOURNAL OF DAIRY SCIENCE, 99(4), 2940–2944.
Chicago author-date
Piccart, Kristine, A Vasquez, Sofie Piepers, Sarne De Vliegher, and TC Olofsson. 2016. “Short Communication: Lactic Acid Bacteria from the Honeybee Inhibit the in Vitro Growth of Mastitis Pathogens.” Journal of Dairy Science 99 (4): 2940–2944.
Chicago author-date (all authors)
Piccart, Kristine, A Vasquez, Sofie Piepers, Sarne De Vliegher, and TC Olofsson. 2016. “Short Communication: Lactic Acid Bacteria from the Honeybee Inhibit the in Vitro Growth of Mastitis Pathogens.” Journal of Dairy Science 99 (4): 2940–2944.
Vancouver
1.
Piccart K, Vasquez A, Piepers S, De Vliegher S, Olofsson T. Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens. JOURNAL OF DAIRY SCIENCE. 2016;99(4):2940–4.
IEEE
[1]
K. Piccart, A. Vasquez, S. Piepers, S. De Vliegher, and T. Olofsson, “Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens,” JOURNAL OF DAIRY SCIENCE, vol. 99, no. 4, pp. 2940–2944, 2016.
@article{7215621,
  abstract     = {Despite the increasing knowledge of prevention and control strategies, bovine mastitis remains one of the most challenging diseases in the dairy industry. This study investigated the antimicrobial activity of 13 species of lactic acid bacteria (LAB), previously isolated from the honey crop of the honeybee, on several mastitis pathogens. The viable LAB were first reintroduced into a sterilized heather honey matrix. More than 20 different bovine mastitis isolates were tested against the mixture of the 13 LAB species in the honey medium using a dual-culture overlay assay. The mastitis isolates were identified through bacteriological culturing, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Additionally, the mastitis isolates were subjected to antimicrobial susceptibility testing through disk diffusion. Growth of all tested mastitis pathogens, including the ones displaying antimicrobial resistance to one or more antimicrobial compounds, were inhibited to some extent by the honey and LAB combination. The antibacterial effect of these LAB opens up new perspectives on alternative treatment and prevention of bovine mastitis.},
  author       = {Piccart, Kristine and Vasquez, A and Piepers, Sofie and De Vliegher, Sarne and Olofsson, TC},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {APIS-MELLIFERA,ANTIBACTERIAL ACTIVITY,honey,lactic acid bacteria,bovine mastitis,BOVINE MASTITIS,DAIRY-COWS,IDENTIFICATION,ANIMALS,ORIGIN,IMPACT,FLORA},
  language     = {eng},
  number       = {4},
  pages        = {2940--2944},
  title        = {Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens},
  url          = {http://dx.doi.org/10.3168/jds.2015-10208},
  volume       = {99},
  year         = {2016},
}

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