Ghent University Academic Bibliography

Advanced

Central administration of obestatin fails to show inhibitory effects on food and water intake in mice

Annemie Van Dijck, Debby Van Dam, Valentijn Vergote UGent, Bart De Spiegeleer UGent, Walter Luyten, Liliane Schoofs and Peter Paul De Deyn (2009) Regulatory Peptides. 156(1-3). p.77-82
abstract
Obestatin is a ghrelin-associated peptide hormone with presumed anorexigenic and inhibitory effect on gastric propulsive motility activity. Recent literature, however, discloses much contestation over satiety and gastrointestinal motility-related functionalities of obestatin. In addition, antidipsinogenic effects in rodents by obestatin were recently reported. The present study was set up to bring more clarity into the contested effects of obestatin on food and water intake. Additionally, the stability of obestatin in brain tissue homogenate was investigated. The in vitro incubation of obestatin in brain homogenates revealed disappearance half-life times of 19 min for crude brain homogenate to 27 min for brain membrane homogenate. For the behavioural studies, male C57Bl/6 mice were intracerebroventricularly treated with 0.2 nmol murine amidated obestatin or vehicle at the age of 3 months. An additional group of mice was treated with 0.3 nmol of corticotropin releasing factor (CRF) as a positive control of suppression of food intake. Food and water intake were studied over a period of 5 h in metabolic cages. Under our experimental conditions, no suppressive effects of obestatin on food or water intake were observed, whereas CRF evoked a significant suppression of food intake, which proves the internal validity of the study design.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Peptide, Intracerebroventricular, Mouse, Eating behaviour, Metabolic brain stability
journal title
Regulatory Peptides
Regul. Pept.
volume
156
issue
1-3
pages
77 - 82
publisher
Elsevier Science BV
place of publication
Amsterdam, The Netherlands
Web of Science type
Article
Web of Science id
000268087900012
JCR category
PHYSIOLOGY
JCR impact factor
2.16 (2009)
JCR rank
38/75 (2009)
JCR quartile
3 (2009)
ISSN
0167-0115
DOI
10.1016/j.regpep.2009.04.014
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
721217
handle
http://hdl.handle.net/1854/LU-721217
date created
2009-08-03 09:35:50
date last changed
2009-08-06 08:14:34
@article{721217,
  abstract     = {Obestatin is a ghrelin-associated peptide hormone with presumed anorexigenic and inhibitory effect on gastric propulsive motility activity. Recent literature, however, discloses much contestation over satiety and gastrointestinal motility-related functionalities of obestatin. In addition, antidipsinogenic effects in rodents by obestatin were recently reported. The present study was set up to bring more clarity into the contested effects of obestatin on food and water intake. Additionally, the stability of obestatin in brain tissue homogenate was investigated. The in vitro incubation of obestatin in brain homogenates revealed disappearance half-life times of 19 min for crude brain homogenate to 27 min for brain membrane homogenate. For the behavioural studies, male C57Bl/6 mice were intracerebroventricularly treated with 0.2 nmol murine amidated obestatin or vehicle at the age of 3 months. An additional group of mice was treated with 0.3 nmol of corticotropin releasing factor (CRF) as a positive control of suppression of food intake. Food and water intake were studied over a period of 5 h in metabolic cages. Under our experimental conditions, no suppressive effects of obestatin on food or water intake were observed, whereas CRF evoked a significant suppression of food intake, which proves the internal validity of the study design.},
  author       = {Van Dijck, Annemie and Van Dam, Debby and Vergote, Valentijn and De Spiegeleer, Bart and Luyten, Walter and Schoofs, Liliane and De Deyn, Peter Paul},
  issn         = {0167-0115},
  journal      = {Regulatory Peptides},
  keyword      = {Peptide,Intracerebroventricular,Mouse,Eating behaviour,Metabolic brain stability},
  language     = {eng},
  number       = {1-3},
  pages        = {77--82},
  publisher    = {Elsevier Science BV},
  title        = {Central administration of obestatin fails to show inhibitory effects on food and water intake in mice},
  url          = {http://dx.doi.org/10.1016/j.regpep.2009.04.014},
  volume       = {156},
  year         = {2009},
}

Chicago
Van Dijck, Annemie, Debby Van Dam, Valentijn Vergote, Bart De Spiegeleer, Walter Luyten, Liliane Schoofs, and Peter Paul De Deyn. 2009. “Central Administration of Obestatin Fails to Show Inhibitory Effects on Food and Water Intake in Mice.” Regulatory Peptides 156 (1-3): 77–82.
APA
Van Dijck, A., Van Dam, D., Vergote, V., De Spiegeleer, B., Luyten, W., Schoofs, L., & De Deyn, P. P. (2009). Central administration of obestatin fails to show inhibitory effects on food and water intake in mice. Regulatory Peptides, 156(1-3), 77–82.
Vancouver
1.
Van Dijck A, Van Dam D, Vergote V, De Spiegeleer B, Luyten W, Schoofs L, et al. Central administration of obestatin fails to show inhibitory effects on food and water intake in mice. Regulatory Peptides. Amsterdam, The Netherlands: Elsevier Science BV; 2009;156(1-3):77–82.
MLA
Van Dijck, Annemie, Debby Van Dam, Valentijn Vergote, et al. “Central Administration of Obestatin Fails to Show Inhibitory Effects on Food and Water Intake in Mice.” Regulatory Peptides 156.1-3 (2009): 77–82. Print.