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Central administration of obestatin fails to show inhibitory effects on food and water intake in mice

(2009) Regulatory Peptides. 156(1-3). p.77-82
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Abstract
Obestatin is a ghrelin-associated peptide hormone with presumed anorexigenic and inhibitory effect on gastric propulsive motility activity. Recent literature, however, discloses much contestation over satiety and gastrointestinal motility-related functionalities of obestatin. In addition, antidipsinogenic effects in rodents by obestatin were recently reported. The present study was set up to bring more clarity into the contested effects of obestatin on food and water intake. Additionally, the stability of obestatin in brain tissue homogenate was investigated. The in vitro incubation of obestatin in brain homogenates revealed disappearance half-life times of 19 min for crude brain homogenate to 27 min for brain membrane homogenate. For the behavioural studies, male C57Bl/6 mice were intracerebroventricularly treated with 0.2 nmol murine amidated obestatin or vehicle at the age of 3 months. An additional group of mice was treated with 0.3 nmol of corticotropin releasing factor (CRF) as a positive control of suppression of food intake. Food and water intake were studied over a period of 5 h in metabolic cages. Under our experimental conditions, no suppressive effects of obestatin on food or water intake were observed, whereas CRF evoked a significant suppression of food intake, which proves the internal validity of the study design.
Keywords
Peptide, Intracerebroventricular, Mouse, Eating behaviour, Metabolic brain stability

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MLA
Van Dijck, Annemie et al. “Central Administration of Obestatin Fails to Show Inhibitory Effects on Food and Water Intake in Mice.” Regulatory Peptides 156.1-3 (2009): 77–82. Print.
APA
Van Dijck, A., Van Dam, D., Vergote, V., De Spiegeleer, B., Luyten, W., Schoofs, L., & De Deyn, P. P. (2009). Central administration of obestatin fails to show inhibitory effects on food and water intake in mice. Regulatory Peptides, 156(1-3), 77–82.
Chicago author-date
Van Dijck, Annemie, Debby Van Dam, Valentijn Vergote, Bart De Spiegeleer, Walter Luyten, Liliane Schoofs, and Peter Paul De Deyn. 2009. “Central Administration of Obestatin Fails to Show Inhibitory Effects on Food and Water Intake in Mice.” Regulatory Peptides 156 (1-3): 77–82.
Chicago author-date (all authors)
Van Dijck, Annemie, Debby Van Dam, Valentijn Vergote, Bart De Spiegeleer, Walter Luyten, Liliane Schoofs, and Peter Paul De Deyn. 2009. “Central Administration of Obestatin Fails to Show Inhibitory Effects on Food and Water Intake in Mice.” Regulatory Peptides 156 (1-3): 77–82.
Vancouver
1.
Van Dijck A, Van Dam D, Vergote V, De Spiegeleer B, Luyten W, Schoofs L, et al. Central administration of obestatin fails to show inhibitory effects on food and water intake in mice. Regulatory Peptides. Amsterdam, The Netherlands: Elsevier Science BV; 2009;156(1-3):77–82.
IEEE
[1]
A. Van Dijck et al., “Central administration of obestatin fails to show inhibitory effects on food and water intake in mice,” Regulatory Peptides, vol. 156, no. 1–3, pp. 77–82, 2009.
@article{721217,
  abstract     = {Obestatin is a ghrelin-associated peptide hormone with presumed anorexigenic and inhibitory effect on gastric propulsive motility activity. Recent literature, however, discloses much contestation over satiety and gastrointestinal motility-related functionalities of obestatin. In addition, antidipsinogenic effects in rodents by obestatin were recently reported. The present study was set up to bring more clarity into the contested effects of obestatin on food and water intake. Additionally, the stability of obestatin in brain tissue homogenate was investigated. The in vitro incubation of obestatin in brain homogenates revealed disappearance half-life times of 19 min for crude brain homogenate to 27 min for brain membrane homogenate. For the behavioural studies, male C57Bl/6 mice were intracerebroventricularly treated with 0.2 nmol murine amidated obestatin or vehicle at the age of 3 months. An additional group of mice was treated with 0.3 nmol of corticotropin releasing factor (CRF) as a positive control of suppression of food intake. Food and water intake were studied over a period of 5 h in metabolic cages. Under our experimental conditions, no suppressive effects of obestatin on food or water intake were observed, whereas CRF evoked a significant suppression of food intake, which proves the internal validity of the study design.},
  author       = {Van Dijck, Annemie and Van Dam, Debby and Vergote, Valentijn and De Spiegeleer, Bart and Luyten, Walter and Schoofs, Liliane and De Deyn, Peter Paul},
  issn         = {0167-0115},
  journal      = {Regulatory Peptides},
  keywords     = {Peptide,Intracerebroventricular,Mouse,Eating behaviour,Metabolic brain stability},
  language     = {eng},
  number       = {1-3},
  pages        = {77--82},
  publisher    = {Elsevier Science BV},
  title        = {Central administration of obestatin fails to show inhibitory effects on food and water intake in mice},
  url          = {http://dx.doi.org/10.1016/j.regpep.2009.04.014},
  volume       = {156},
  year         = {2009},
}

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