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Wound-healing markers after autologous and allogeneic epithelial-like stem cell treatment

Jan H Spaas, Chiara Gomiero, Sarah Broeckx, Lore Van Hecke, Lisa Maccatrozzo, Ann Martens UGent, Tiziana Martinello and Marco Patruno (2016) CYTOTHERAPY. 18(4). p.562-569
abstract
Background aims. Several cytokines and growth factors play an essential role in skin regeneration and epithelial-like stem cells (EpSCs) have beneficial effects on wound healing in horses. However, there are no reports available on the expression of these growth factors and cytokines after EpSC therapy. Methods. Wounds of 6 cm2 were induced in the gluteus region of 6 horses and treated with (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Real time polymerase chain reaction was performed on tissue biopsies taken 1 and 5 weeks after these treatments to evaluate mRNA expression of interferon (IFN)-gamma, interleukin (IL)-6, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), insulin-like growth factor (IGF)-1 and epidermal keratin (eKER). Results. One week after treatments, mRNA levels of IL-6 (P = 0.012) and VEGF (P = 0.008) were higher in allogeneic EpSC-treated wounds compared with controls. Also, mRNA levels of IGF-1 were higher at 1 week in both autologous (P = 0.027) and allogeneic (P = 0.035) EpSC-treated wounds. At week 5, all EpSC- and vehicle-treated wounds demonstrated significantly higher IFN-gamma, VEGF and eKER mRNA expression compared with controls and compared with their respective levels at week 1. Conclusions. Equine wounds treated with allogeneic EpSCs demonstrate a significant increase in mRNA expression of IL-6, VEGF and IGF-1 in the acute phase. In the longer term, an increase in IFN-gamma, VEGF and eKER mRNA was detected in the wounds treated with allogenic EpSCs, autologous EpSCs or their vehicle.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
autologous, allogeneic, cytokines, growth factors, horse, skin, stem cells, EPIDERMAL-GROWTH-FACTOR, IN-VITRO, CUTANEOUS WOUNDS, EQUINE SKIN, REPAIR, TISSUE, KERATINOCYTES, ACTIVATION, MIGRATION, CAPACITY
journal title
CYTOTHERAPY
Cytotherapy
volume
18
issue
4
pages
562 - 569
Web of Science type
Article
Web of Science id
000372101400009
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.203 (2016)
JCR rank
47/158 (2016)
JCR quartile
2 (2016)
ISSN
1465-3249
DOI
10.1016/j.jcyt.2016.01.008
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7205936
handle
http://hdl.handle.net/1854/LU-7205936
date created
2016-05-10 11:15:49
date last changed
2016-12-19 15:47:27
@article{7205936,
  abstract     = {Background aims. Several cytokines and growth factors play an essential role in skin regeneration and epithelial-like stem cells (EpSCs) have beneficial effects on wound healing in horses. However, there are no reports available on the expression of these growth factors and cytokines after EpSC therapy.
Methods. Wounds of 6 cm2 were induced in the gluteus region of 6 horses and treated with (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Real time polymerase chain reaction was performed on tissue biopsies taken 1 and 5 weeks after these treatments to evaluate mRNA expression of interferon (IFN)-gamma, interleukin (IL)-6, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), insulin-like growth factor (IGF)-1 and epidermal keratin (eKER).
Results. One week after treatments, mRNA levels of IL-6 (P = 0.012) and VEGF (P = 0.008) were higher in allogeneic EpSC-treated wounds compared with controls. Also, mRNA levels of IGF-1 were higher at 1 week in both autologous (P = 0.027) and allogeneic (P = 0.035) EpSC-treated wounds. At week 5, all EpSC- and vehicle-treated wounds demonstrated significantly higher IFN-gamma, VEGF and eKER mRNA expression compared with controls and compared with their respective levels at week 1.
Conclusions. Equine wounds treated with allogeneic EpSCs demonstrate a significant increase in mRNA expression of IL-6, VEGF and IGF-1 in the acute phase. In the longer term, an increase in IFN-gamma, VEGF and eKER mRNA was detected in the wounds treated with allogenic EpSCs, autologous EpSCs or their vehicle.},
  author       = {Spaas, Jan H and Gomiero, Chiara and Broeckx, Sarah and Van Hecke, Lore and Maccatrozzo, Lisa and Martens, Ann and Martinello, Tiziana and Patruno, Marco},
  issn         = {1465-3249},
  journal      = {CYTOTHERAPY},
  keyword      = {autologous,allogeneic,cytokines,growth factors,horse,skin,stem cells,EPIDERMAL-GROWTH-FACTOR,IN-VITRO,CUTANEOUS WOUNDS,EQUINE SKIN,REPAIR,TISSUE,KERATINOCYTES,ACTIVATION,MIGRATION,CAPACITY},
  language     = {eng},
  number       = {4},
  pages        = {562--569},
  title        = {Wound-healing markers after autologous and allogeneic epithelial-like stem cell treatment},
  url          = {http://dx.doi.org/10.1016/j.jcyt.2016.01.008},
  volume       = {18},
  year         = {2016},
}

Chicago
Spaas, Jan H, Chiara Gomiero, Sarah Broeckx, Lore Van Hecke, Lisa Maccatrozzo, Ann Martens, Tiziana Martinello, and Marco Patruno. 2016. “Wound-healing Markers After Autologous and Allogeneic Epithelial-like Stem Cell Treatment.” Cytotherapy 18 (4): 562–569.
APA
Spaas, J. H., Gomiero, C., Broeckx, S., Van Hecke, L., Maccatrozzo, L., Martens, A., Martinello, T., et al. (2016). Wound-healing markers after autologous and allogeneic epithelial-like stem cell treatment. CYTOTHERAPY, 18(4), 562–569.
Vancouver
1.
Spaas JH, Gomiero C, Broeckx S, Van Hecke L, Maccatrozzo L, Martens A, et al. Wound-healing markers after autologous and allogeneic epithelial-like stem cell treatment. CYTOTHERAPY. 2016;18(4):562–9.
MLA
Spaas, Jan H, Chiara Gomiero, Sarah Broeckx, et al. “Wound-healing Markers After Autologous and Allogeneic Epithelial-like Stem Cell Treatment.” CYTOTHERAPY 18.4 (2016): 562–569. Print.