Ghent University Academic Bibliography

Advanced

Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization

Na Liu, Zhiyong Zhao, Yanglan Tan, Lei Lu, Lin Wang, Yucai Liao, Natalia Beloglazova UGent, Sarah De Saeger UGent, Xiaodong Zheng and Aibo Wu (2016) ANALYTICAL CHEMISTRY. 88(2). p.1246-1252
abstract
Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GOLD, CANCER, HAPTENS, RESIDUES, ASSAY, PROTEINS, RAW-MILK, INDIRECT COMPETITIVE ELISA, LATERAL FLOW IMMUNOASSAY, EGG
journal title
ANALYTICAL CHEMISTRY
Anal. Chem.
volume
88
issue
2
pages
1246 - 1252
Web of Science type
Article
Web of Science id
000368562300028
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
6.32 (2016)
JCR rank
4/76 (2016)
JCR quartile
1 (2016)
ISSN
0003-2700
DOI
10.1021/acs.analchem.5b03637
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7205677
handle
http://hdl.handle.net/1854/LU-7205677
date created
2016-05-09 17:14:02
date last changed
2016-12-19 15:48:38
@article{7205677,
  abstract     = {Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.},
  author       = {Liu, Na and Zhao, Zhiyong and Tan, Yanglan and Lu, Lei and Wang, Lin and Liao, Yucai and Beloglazova, Natalia and De Saeger, Sarah and Zheng, Xiaodong and Wu, Aibo},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {GOLD,CANCER,HAPTENS,RESIDUES,ASSAY,PROTEINS,RAW-MILK,INDIRECT COMPETITIVE ELISA,LATERAL FLOW IMMUNOASSAY,EGG},
  language     = {eng},
  number       = {2},
  pages        = {1246--1252},
  title        = {Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization},
  url          = {http://dx.doi.org/10.1021/acs.analchem.5b03637},
  volume       = {88},
  year         = {2016},
}

Chicago
Liu, Na, Zhiyong Zhao, Yanglan Tan, Lei Lu, Lin Wang, Yucai Liao, Natalia Beloglazova, Sarah De Saeger, Xiaodong Zheng, and Aibo Wu. 2016. “Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization.” Analytical Chemistry 88 (2): 1246–1252.
APA
Liu, Na, Zhao, Z., Tan, Y., Lu, L., Wang, L., Liao, Y., Beloglazova, N., et al. (2016). Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization. ANALYTICAL CHEMISTRY, 88(2), 1246–1252.
Vancouver
1.
Liu N, Zhao Z, Tan Y, Lu L, Wang L, Liao Y, et al. Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization. ANALYTICAL CHEMISTRY. 2016;88(2):1246–52.
MLA
Liu, Na, Zhiyong Zhao, Yanglan Tan, et al. “Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization.” ANALYTICAL CHEMISTRY 88.2 (2016): 1246–1252. Print.