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Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization

(2016) ANALYTICAL CHEMISTRY. 88(2). p.1246-1252
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Abstract
Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.
Keywords
GOLD, CANCER, HAPTENS, RESIDUES, ASSAY, PROTEINS, RAW-MILK, INDIRECT COMPETITIVE ELISA, LATERAL FLOW IMMUNOASSAY, EGG

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Chicago
Liu, Na, Zhiyong Zhao, Yanglan Tan, Lei Lu, Lin Wang, Yucai Liao, Natalia Beloglazova, Sarah De Saeger, Xiaodong Zheng, and Aibo Wu. 2016. “Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization.” Analytical Chemistry 88 (2): 1246–1252.
APA
Liu, Na, Zhao, Z., Tan, Y., Lu, L., Wang, L., Liao, Y., Beloglazova, N., et al. (2016). Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization. ANALYTICAL CHEMISTRY, 88(2), 1246–1252.
Vancouver
1.
Liu N, Zhao Z, Tan Y, Lu L, Wang L, Liao Y, et al. Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization. ANALYTICAL CHEMISTRY. 2016;88(2):1246–52.
MLA
Liu, Na, Zhiyong Zhao, Yanglan Tan, et al. “Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization.” ANALYTICAL CHEMISTRY 88.2 (2016): 1246–1252. Print.
@article{7205677,
  abstract     = {Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.},
  author       = {Liu, Na and Zhao, Zhiyong and Tan, Yanglan and Lu, Lei and Wang, Lin and Liao, Yucai and Beloglazova, Natalia and De Saeger, Sarah and Zheng, Xiaodong and Wu, Aibo},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {GOLD,CANCER,HAPTENS,RESIDUES,ASSAY,PROTEINS,RAW-MILK,INDIRECT COMPETITIVE ELISA,LATERAL FLOW IMMUNOASSAY,EGG},
  language     = {eng},
  number       = {2},
  pages        = {1246--1252},
  title        = {Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization},
  url          = {http://dx.doi.org/10.1021/acs.analchem.5b03637},
  volume       = {88},
  year         = {2016},
}

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